LOCUS       sequence-351592-        3075 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            Ste2 gene of Candida albicans (receiver part)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK019
FEATURES             Location/Qualifiers
     source          1..3075
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1120..1154)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1225..1884)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1799..1818
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2066..2654
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2555..2574
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacaacagca gcagcaacag caaattgaat caagttctta taacaacacc acaactacta
       61 cttctggatc atacaaactg tttctatttt atttgaattc attgattttg cttattggca
      121 ttataagaag tggttgttat ttaaactata atttgggtcc attaaattcc ttatcctttg
      181 tttttactgg ttggtatgat ggttcctcat tcatatcttc tgatgttact aatggcttca
      241 aatgtatttt gtatgctttg gttgaaattt ctttgggatt ccaggtatac gtcatgttta
      301 agacttctaa cttaaaaata tggggtatta tggcttcact tttgagtatc gggttaggac
      361 tcattgttgt tgcctttcaa ataaatttaa caatattgtc gcatattaga ttttctagag
      421 ctatttcaac taacaggtca gaggaagaaa gtagttcatc gctatcatca gattcagttg
      481 gttatgttat caactctatt tggatggatt taccaactat attattctcc attagtatta
      541 atatcatgac aattttgtta atcggtaaat tgataattgc tatcagaaca agaagatact
      601 taggtttgaa acaatttgat tcttttcata tcttgctaat tggtttttct caaaccttga
      661 tcattccttc aattatcttg gtcgtacatt atttttacct gtcacaaaat aaagattcat
      721 tgcttcaaca gatttcttta ttacttatta ttttaatgct tccattgtct tcattatggg
      781 ctcaaactgc taataacact cataatataa attcgtcccc atcattgagt tttatttcaa
      841 gacatcattc atttgatagc agtagatcag gtggttctaa tactattgta tctaacggtg
      901 gctcaaatgg tgggggcggc ggtggtaact ttcctgtttc tggtattgat gcacaattgc
      961 ctccagatat agaaaagatt ctccatgaag ataacaatta taaattactg aatagtaata
     1021 atgaatctgt gaatgacggt gatattatta ttaacgatga aggtatgatt acaaagcaga
     1081 tcaccatcaa aagagtttga ggatcctgag accagaccaa taaaaaacgc ccggcggcaa
     1141 ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg
     1201 agtccaagcg agctcgatat caaattacgc cccgccctgc cactcatcgc agtactgttg
     1261 taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat
     1321 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
     1381 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
     1441 attggctgaa acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
     1501 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
     1561 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
     1621 aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc
     1681 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt
     1741 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc
     1801 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt
     1861 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc
     1921 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg
     1981 cccgatcaat catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     2041 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     2101 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2161 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     2221 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     2281 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     2341 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     2401 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     2461 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     2521 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     2581 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     2641 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     2701 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtagtcggt
     2761 ctcatatgaa tattaattct actttcatcc ctgataaacc aggcgatatc atcatatcat
     2821 attctatccc aggtttggat caacctattc aaattccatt ccatagtttg gactctttcc
     2881 aaacagacca agctaaaatt gcattagtta tgggtataac cattggaagc tgttctatga
     2941 ctctgatttt tttaatatcc ataatgtaca agacaaacaa attgacaaat ctaaaattga
     3001 agttaaagtt gaaatatatc ttacaatgga tcaatcaaaa aatctttacc aagaaaagga
     3061 atgataataa gcaac
//
