LOCUS       sequence-351593-        2163 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            alpha-factor gene of Saccharomyces cerevisiae (sender part)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK016
FEATURES             Location/Qualifiers
     source          1..2163
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(44..100,101..298,299..310)
                     /codon_start=1
                     /gene="MF-alpha-1"
                     /product="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /label=alpha-factor secretion signal
                     /note="Cleavage by the Kex2 protease occurs after the 
                     dibasic KR sequence. The EA dipeptides are then removed by 
                     dipeptidyl aminopeptidase A."
                     /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYLDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLDKREAEA"
     terminator      complement(561..595)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(666..1325)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1240..1259
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1507..2095
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1996..2015
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgcgttatcc cctgattctg tggataaccg tagtcggtct catatgagat ttccttcaat
       61 ttttactgca gttttattcg cagcatcctc cgcattagct gctccagtca acactacaac
      121 agaagatgaa acggcacaaa ttccggctga agctgtcatc ggttacttag atttagaagg
      181 ggatttcgat gttgctgttt tgccattttc caacagcaca aataacgggt tattgtttat
      241 aaatactact attgccagca ttgctgctaa agaagaaggg gtatctttgg ataaaagaga
      301 ggctgaagct tggcattggt tgcaactaaa acctggccaa ccaatgtaca agagagaagc
      361 cgaagctgaa gcttggcatt ggctgcaact aaagcctggc caaccaatgt acaaaagaga
      421 agccgacgct gaagcttggc attggctgca actaaagcct ggccaaccaa tgtacaaaag
      481 agaagccgac gctgaagctt ggcattggtt gcagttaaaa cccggccaac caatgtacta
      541 aggatcctga gaccagacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat
      601 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata
      661 tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
      721 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
      781 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
      841 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga aacgaaaaac
      901 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
      961 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     1021 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
     1081 agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga
     1141 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     1201 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     1261 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
     1321 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
     1381 gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa tcatgaccaa
     1441 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     1501 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1561 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1621 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     1681 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1741 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1801 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1861 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     1921 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     1981 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2041 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     2101 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     2161 tcc
//
