LOCUS       sequence-351594-        2091 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            alpha-factor gene of Lachancea fermentati (sender part)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK013
FEATURES             Location/Qualifiers
     source          1..2091
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(465..499)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(570..1229)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1144..1163
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1411..1999
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1900..1919
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taaccgtagt cggtctcata tgcaaagatt attaatttta attttaggtt tggcttctac
       61 tttaatgtct gtttctgctg caccattaac tgatgcagct tctactgttg atattccaca
      121 agaatctatt ttgggtgttt ttgaaatttc tgattatgat gatattgctt tattaccagt
      181 ttcaaatggt actcattctg ctgttttgat tttgaatgct actttgattg atgctgctgg
      241 tacttctaat tctgatgaag ttgaaaaaag atctgcttct ccttggcact ggttaagatt
      301 aaaaggccct atgtacaaaa gggatgcttc tccacaagct gaaccatggc attggttgag
      361 attgaaaggt ccaatgtata aaagagatgc taatgccgac gctgatccat ggcattggct
      421 aagattgaag ggtcccatgt attaaggatc ctgagaccag accaataaaa aacgcccggc
      481 ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca
      541 acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac
      601 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc
      661 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa
      721 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
      781 cagggattgg ctgaaacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
      841 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
      901 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
      961 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga
     1021 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
     1081 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
     1141 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
     1201 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat
     1261 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
     1321 acgtgcccga tcaatcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1381 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1441 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     1501 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1561 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1621 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1681 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1741 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1801 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1861 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     1921 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     1981 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2041 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg a
//
