LOCUS       sequence-351598-        2184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            alpha-factor gene of Eremothecium cymbalariae (sender part)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK009
FEATURES             Location/Qualifiers
     source          1..2184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(321..355)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(426..1085)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1000..1019
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1267..1855
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1756..1775
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgctagatct gaacaagctg ctggtatttc taaaagagat gctgatgctt ggcattggct
       61 aagatttgat agaggccaac caatccataa aagaagtgct gatgcagttg cagatgcttg
      121 gcactggtta agattcgatc gtggacaacc aatacataaa aggtctgcag acgctgttgc
      181 cgatgcctgg cattggctta ggtttgatag gggtcagcca attcacaaaa gatctgctga
      241 cgcagtagct gatgcatggc actggttgag atttgacaga ggtcaaccta ttcataagta
      301 aggatcctga gaccagacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat
      361 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata
      421 tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
      481 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
      541 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
      601 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga aacgaaaaac
      661 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
      721 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
      781 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
      841 agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga
      901 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
      961 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     1021 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
     1081 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
     1141 gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa tcatgaccaa
     1201 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     1261 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1321 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1381 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     1441 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1501 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1561 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1621 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     1681 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     1741 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     1801 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     1861 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     1921 tcctgcgtta tcccctgatt ctgtggataa ccgtagtcgg tctcatatga aattttataa
     1981 tattttatct gttgcttcta ttgcatcttt agtttttgct gctccagttt ctgttaatga
     2041 tgccaaagaa attgctgcaa cttttccaca agaagcttta ttaggttttt tggatttaac
     2101 tgatgctgaa aatattgtta ttttgtcttt ggttgatgaa gaaaaatctg gtattgcttt
     2161 ggttaataaa actatttggg ctac
//
