LOCUS       sequence-351599-        2194 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            alpha-factor-responsive promoter YPS1 (response part)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK007
FEATURES             Location/Qualifiers
     source          1..2194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     147..166
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(906..940)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1011..1670)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1585..1604
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(1852..2194,1..246)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
       61 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
      121 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
      181 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
      241 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
      301 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtagtcg gtctcaaacg
      361 atgtggtagg taatattata ctatatgctt ggtgttcacc ttatcttttt attacgctat
      421 gtattttaat atacagaaaa gccaattgcg ttcttcttaa taaccgtcca attgcggaaa
      481 taggaacaaa tgcaaaaaga aggaaaaaag taaatcctct ttgacaccct tttcttaagt
      541 agtcggattt ccctgtttct ccataaataa gtccacgaat ttttttatga aacctcaatt
      601 ttttcggaca catcagtgaa acgaaaacta ctcatggcgc agtataaaag gagcgaaaag
      661 ccactcagtt gagtggacga gggaggtgag gtggtgacag aaaagaagag gaaggaggca
      721 gtgctatgtg agggtgtctt ggctgcatgc aaggtctgac gatctaattg acgcgtcaga
      781 acgtgtgttt taggaatatt cttatttaag tatgcttttc ctcttgaaat cgcgcaaaga
      841 aggaaaggaa aaagtgtcta tatatagtgt ctattgtttt tttagatcta tgtgagacca
      901 gaccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg
      961 aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg
     1021 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca
     1081 tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
     1141 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
     1201 tcaaaactgg tgaaactcac ccagggattg gctgaaacga aaaacatatt ctcaataaac
     1261 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt
     1321 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc
     1381 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc
     1441 attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc
     1501 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga
     1561 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga
     1621 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc
     1681 ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta
     1741 tggtgaaagt tggaacctct tacgtgcccg atcaatcatg accaaaatcc cttaacgtga
     1801 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     1861 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     1921 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     1981 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     2041 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     2101 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     2161 gtcgggctga acggggggtt cgtgcacaca gccc
//
