LOCUS       sequence-351600-        2195 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            alpha-factor-responsive promoter DIG2 of S. cerevisiae (response 
            part).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK001
FEATURES             Location/Qualifiers
     source          1..2195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     48..67
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(808..842)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(913..1572)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1487..1506
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(1754..2195,1..147)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
       61 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
      121 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
      181 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
      241 aaccgtagtc ggtctcaaac gctagacgta tatacagtag gagactgaaa ttttgctacg
      301 tatacaggtg agtatattca tgcggcctga tcagtaatta aaaatacaca ctattcctcg
      361 ttgttatttc ttaaaattag ttcaagaaga aggggtaacc cctaagcttc ttaagttcac
      421 cacaaaagta ttactggata gtgaaacaac aaccacccat aacattagtt tcctaaacac
      481 tacggcggta ttcacctaga cgccatccta gaaggctaca aagtgatgca cgtatgatga
      541 tatcggacat tagagcgtat acatacaaca ggtacacata aaattgtgat atgtcacagc
      601 ttcatcaccc ggcggacacg tctattaaat tatcaataat tacataatgt gcagatcaaa
      661 taaacaataa acatacagcc atttaaattt tggttattaa tgttattttt catttctatt
      721 gcttgcagtg gtatttatgt tgtatagtta agcctgcagg aatatttaag caaagaggga
      781 aaattagatc tatgtgagac cagaccaata aaaaacgccc ggcggcaacc gagcgttctg
      841 aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag
      901 ctcgatatca aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag
      961 cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat
     1021 cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt
     1081 gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgaaac
     1141 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc
     1201 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
     1261 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
     1321 tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg
     1381 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
     1441 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
     1501 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat
     1561 ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc
     1621 cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaatca
     1681 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     1741 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     1801 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     1861 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     1921 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     1981 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2041 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2101 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     2161 cgcttcccga agggagaaag gcggacaggt atccg
//
