LOCUS       sequence-351601-        8903 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses diffuse K560-mCherry-eDHFR .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8903)
  AUTHORS   Ballister ER, Ayloo S, Chenoweth DM, Lampson MA, Holzbaur EL
  TITLE     Optogenetic control of organelle transport using a photocaged 
            chemical inducer of dimerization.
  JOURNAL   Curr Biol. 2015 May 18;25(10):R407-8. doi: 
            10.1016/j.cub.2015.03.056.
  PUBMED    25989077
REFERENCE   2  (bases 1 to 8903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2015.03"; journalName: "Curr Biol"; date: 
            "2015-05-18- 18"; volume: "25"; issue: "10"; pages: "R407-8"
COMMENT     Sequence Label: pERB258
FEATURES             Location/Qualifiers
     source          1..8903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        306..583
                     /label=chicken beta-actin promoter
     intron          584..1600
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1524..1546
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1608..1627
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3365..4072
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3510..3528)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3778..3797
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4091..4564
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="ISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHT
                     WESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     CDS             4565..4594
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(4701..4720)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4766..4821
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4820..4839)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     protein_bind    5185..5218
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    5231..5352
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5268..5287)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5322..5341
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5371..5391)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5371..5389)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5410..5426)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5410..5426)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5423..5445)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5434..5450
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5458..5464,5465..5482,5483..5488))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5503..5524
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5641..5658)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5812..6400)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5892..5911)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6571..7362,7363..7431))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7194..7213
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7432..7536)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7609..7657
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    7869..7902
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             7907..8506
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7907..7926)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8403..8423
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(8642..8659)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8648..8759
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
      361 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
      421 gggggggcgc gcgccrggsg gggsggggsg gggsgrgggg sggggsgggg sgaggcggag
      481 aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg
      541 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg
      601 ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac
      661 cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg
      721 cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg
      781 ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg
      841 gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg
      901 gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg
      961 cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc
     1021 agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc cccgagttgc
     1081 tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt
     1141 gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc ctcgggccgg
     1201 ggagggctcg ggggaggggc gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc
     1261 gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc
     1321 ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg
     1381 ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc
     1441 gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg acggctgcct
     1501 tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc
     1561 ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt
     1621 tattgtgctg tctcatcatt ttggcaaaga attgctcgac caccggcgcg ccatggcgga
     1681 cctggccgag tgcaacatca aagtgatgtg tcgcttcaga cctctcaacg agtctgaagt
     1741 gaaccgcggc gacaagtaca tcgccaagtt tcagggagaa gacacggtcg tgatcgcgtc
     1801 caagccttat gcatttgatc gggtgttcca gtcaagcaca tctcaagagc aagtgtataa
     1861 tgactgtgca aagaagattg ttaaagatgt acttgaagga tataatggaa caatatttgc
     1921 atatggacaa acatcctctg ggaagacaca cacaatggag ggtaaacttc atgatccaga
     1981 gggcatggga attattccaa gaatagtgca agatattttt aattatattt actccatgga
     2041 tgaaaatttg gaatttcata ttaaggtttc atattttgaa atatatttgg ataagataag
     2101 ggacctgtta gatgtttcaa agaccaacct ttcagttcat gaagacaaaa accgagttcc
     2161 ctatgtaaag gggtgcacag agcgttttgt atgtagtcca gatgaagtta tggataccat
     2221 agatgaagga aaatccaaca gacatgtagc agttacaaat atgaatgaac atagctctag
     2281 gagtcacagt atatttctta ttaatgtcaa acaagagaac acacaaacgg aacaaaagct
     2341 gagtggaaaa ctttatctgg ttgatttagc tggtagtgaa aaggttagta aaactggagc
     2401 tgaaggtgct gtgctggatg aagctaaaaa catcaacaag tcactttctg ctcttggaaa
     2461 tgttatttct gctttggctg agggtagtac acatgttcca tatcgagata gtaaaatgac
     2521 aagaatcctt caagattcat taggtggcaa ctgtagaacc actattgtaa tttgctgctc
     2581 tccatcatca tacaatgagt ctgaaacaaa atctacactc ttatttggcc aaagggccaa
     2641 aacaattaag aacacagttt gtgtcaatgt ggagttaact gcagaacagt ggaaaaagaa
     2701 gtatgaaaaa gaaaaagaaa aaaataagat cctgcggaac actattcagt ggcttgaaaa
     2761 tgagctcaac agatggcgta atggggagac ggtgcctatt gatgaacagt ttgacaaaga
     2821 gaaagccaac ttggaagctt tcacagtgga taaagatatt acttttacca atgataaacc
     2881 agcaaccgca attggagtta taggaaattt tactgatgct gaaagaagaa tgtgtgaaga
     2941 agaaattgct aaattataca aacagcttga tgacaaggat gaagaaatta accagcaaag
     3001 tcaactggta gagaaactga agacgcaaat gttggatcag gaggagcttt tggcatctac
     3061 cagaagggat caagacaata tgcaagctga gctgaatcgc cttcaagcag aaaatgatgc
     3121 ctctaaagaa gaagtgaaag aagttttaca ggccctagaa gaacttgctg tcaattatga
     3181 tcagaagtct caggaagttg aagacaaaac taaggaatat gaattgctta gtgatgaatt
     3241 gaatcagaaa tcggcaactt tagcgagtat agatgctgag cttcagaaac ttaaggaaat
     3301 gaccaaccac cagaaaaaac gagcagctga gatgatggca tctttactaa aaggaccggt
     3361 caccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
     3421 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     3481 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
     3541 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
     3601 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
     3661 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     3721 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     3781 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     3841 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     3901 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     3961 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     4021 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtata aggccggcct
     4081 ggagtctaga atcagtctga ttgcggcgtt agcggtagat cgcgttatcg gcatggaaaa
     4141 cgccatgccg tggaacctgc ctgccgatct cgcctggttt aaacgcaaca ccttaaataa
     4201 acccgtgatt atgggccgcc atacctggga atcaatcggt cgtccgttgc caggacgcaa
     4261 aaatattatc ctcagcagtc aaccgggtac ggacgatcgc gtaacgtggg tgaagtcggt
     4321 ggatgaagcc atcgcggcgt gtggtgacgt accagaaatc atggtgattg gcggcggtcg
     4381 cgtttatgaa cagttcttgc caaaagcgca aaaactgtat ctgacgcata tcgacgcaga
     4441 agtggaaggc gacacccatt tcccggatta cgagccggat gactgggaat cggtattcag
     4501 cgaattccac gatgctgatg cgcagaactc tcacagctat tgctttgaga ttctggagcg
     4561 gcgggaacaa aaactcatct cagaagagga tctttaatta agcgctcgag gcggccgcaa
     4621 ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg
     4681 gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag
     4741 ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt
     4801 tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat
     4861 cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac
     4921 aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta
     4981 ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt
     5041 ttttctttaa catccctaaa attttcctta catgttttac tagccagatt tttcctcctc
     5101 tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct cgacctgcac
     5161 cgtcgagcta gcatatggat ccatataact tcgtataatg tatgctatac gaagttatta
     5221 cgataccgtc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     5281 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     5341 caatgtatct taggtaccca gcttttgttc cctttagtga gggttaattg cgcgcttggc
     5401 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     5461 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     5521 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     5581 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     5641 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     5701 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     5761 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     5821 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     5881 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     5941 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     6001 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     6061 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     6121 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     6181 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     6241 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     6301 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     6361 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     6421 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     6481 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     6541 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     6601 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     6661 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     6721 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     6781 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     6841 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     6901 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     6961 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     7021 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     7081 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     7141 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     7201 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     7261 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     7321 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     7381 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     7441 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     7501 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccaag
     7561 cttaataaac acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa
     7621 gacagaataa aacgcacggg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
     7681 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
     7741 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
     7801 ggggcggcag gccctgccat agccactggc cccgtgggtt agggacgggg tcccccattc
     7861 gagaataaat aacttcgtat aaagtatcct atacgaagtt atcaccatga ccgagtacaa
     7921 gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc
     7981 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg
     8041 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     8101 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     8161 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     8221 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     8281 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     8341 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     8401 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     8461 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga
     8521 cccgcagcgc ccgaccgaaa ggagcgcacg accccatggc tccgaccgaa gccacccggg
     8581 gcggccccgc cgaccccgca cccgcccccg aggcccaccg actctagagc tcgctgatca
     8641 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     8701 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     8761 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     8821 gaggattggg aagacaatag cactagttat taatagtaat caattacggg gtcattagtt
     8881 catagcccat atatggagtt ccg
//
