LOCUS       sequence-351621-        5859 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid containing the SF3B3-Xon/Firefly luciferase cassette. 
            Translation of Firefly luciferase is controlled by splicing 
            modulation of the SF3B3-xon by LMI070..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5859)
  AUTHORS   Monteys AM, Hundley AA, Ranum PT, Tecedor L, Muehlmatt A, Lim E, 
            Lukashev D, Sivasankaran R, Davidson BL
  TITLE     Regulated control of gene therapies by drug-induced splicing.
  JOURNAL   Nature. 2021 Jul 28. pii: 10.1038/s41586-021-03770-2. doi: 
            10.1038/s41586-021-03770-2.
  PUBMED    34321659
REFERENCE   2  (bases 1 to 5859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 Jul 28. pii: 10.1038/s41586-021-03770-2. doi: 
            10.1038/s41586-021-03770-2."
COMMENT     Sequence Label: pGL4.10/RSV_SF3B3-Xon_Luciferase
FEATURES             Location/Qualifiers
     source          1..5859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        196..458
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     regulatory      1580..1589
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1683..3332
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAH
                     IEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPA
                     NDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSM
                     YTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHAR
                     DPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKI
                     QSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG
                     LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG
                     YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL
                     QHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVF
                     VDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    3376..3497
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3387..3406)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3441..3460
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3749..3766)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3920..4508)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4000..4019)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4708..5499,5500..5568))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5673..5721
                     /note="synthetic polyadenylation signal"
     misc_feature    5735..5826
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5775..5794
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 tcgagcctgc aggcgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
       61 ttcgcgatgt acgggccaga tatacgcgta tctgagggga ctagggtgtg tttaggcgaa
      121 aagcggggct tcggttgtac gcggttagga gtcccctcag gatatagtag tttcgctttt
      181 gcatagggag ggggaaatgt agtcttatgc aatacacttg tagtcttgca acatggtaac
      241 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
      301 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acaggtctga catggattgg
      361 acgaaccact gaattgcgca ttgcagagat aattgtattt aagtgcctag ctcgatacaa
      421 taaacgccat ttgaccattc accacattgg tgtgcacctc caaggccaca gatccgttta
      481 aacaagcttg gcaatccggt actgttggta aatttctgta caacttaacc ttgcagagag
      541 ccactggcat cagctttgcc attcttggaa acttttctgg taagttctct cgttaccatc
      601 ttttgaaatt ttaagtgaat taatacatat cttgcttagt ctcttgtgca ggaaatgttt
      661 tccatttatg acaaaacagt tgttggtaaa tgtaaacaat tcaaattatg agatgaggtg
      721 ttaagttctg gtgcattatc tgttacctat ttcagatgca tttcctagtt cacaaattgt
      781 gtaatgattc ttgtcagggc acacttttct tggctgctta cctagtgtgg tttggtgctt
      841 gaagagatag aacttttgta tgagagaaaa gggaaaatgc agtttaggta gggagtgctg
      901 tctgcataga aacagataat ttgcttacgt ttaccatgtg gggaatattt ttgtaaagat
      961 ggattaaggc taggtttgaa ttgtgtgaaa tttcaaatat tggattagga aatacaaagt
     1021 tactgaaagt gaggtactaa tgtttataaa ataaaaactt tttcttgcca tttgcagatt
     1081 taacattttt gagtcaatcc aagtgccacc atgcaggagg ttcatgattg tgtagagtaa
     1141 gacataattt tgttgaggtt taactctgaa tacttaatgt ggtactgaat acttaatgtg
     1201 gtactgagag gcagcctaac tgaccacaca gcattcatat ttcatgttgt tattttctct
     1261 gatcctcatt atggctcttc ctgcgggttt gagtcttcat ttgggtaatt tagttattct
     1321 ttgctgcatt tgattaagga aatacgtata tgggaatttg gagatttgtg actgacttca
     1381 ctatgtctaa tttttttttg agacggagtt ttgctctgtt gcccaggccg gagtgcagtg
     1441 gcacagtctt ggctcacagc aacctctacc tcctgggttc aagcgattct cctgccttag
     1501 cctcccgagt agctgggatt acaggcatgt gccaccacgc ctggctaatt tttgtatttt
     1561 tagtagagac ggggtttcac catggtggcc aggctgggtt ctggttgttt atgatcttta
     1621 ttttttggtg atctaggaac caaacaacaa gaaattgttg tttcccgtgg gtaagaggat
     1681 ccgaagatgc caaaaacatt aagaagggcc cagcgccatt ctacccactc gaagacggga
     1741 ccgccggcga gcagctgcac aaagccatga agcgctacgc cctggtgccc ggcaccatcg
     1801 cctttaccga cgcacatatc gaggtggaca ttacctacgc cgagtacttc gagatgagcg
     1861 ttcggctggc agaagctatg aagcgctatg ggctgaatac aaaccatcgg atcgtggtgt
     1921 gcagcgagaa tagcttgcag ttcttcatgc ccgtgttggg tgccctgttc atcggtgtgg
     1981 ctgtggcccc agctaacgac atctacaacg agcgcgagct gctgaacagc atgggcatca
     2041 gccagcccac cgtcgtattc gtgagcaaga aagggctgca aaagatcctc aacgtgcaaa
     2101 agaagctacc gatcatacaa aagatcatca tcatggatag caagaccgac taccagggct
     2161 tccaaagcat gtacaccttc gtgacttccc atttgccacc cggcttcaac gagtacgact
     2221 tcgtgcccga gagcttcgac cgggacaaaa ccatcgccct gatcatgaac agtagtggca
     2281 gtaccggatt gcccaagggc gtagccctac cgcaccgcac cgcttgtgtc cgattcagtc
     2341 atgcccgcga ccccatcttc ggcaaccaga tcatccccga caccgctatc ctcagcgtgg
     2401 tgccatttca ccacggcttc ggcatgttca ccacgctggg ctacttgatc tgcggctttc
     2461 gggtcgtgct catgtaccgc ttcgaggagg agctattctt gcgcagcttg caagactata
     2521 agattcaatc tgccctgctg gtgcccacac tatttagctt cttcgctaag agcactctca
     2581 tcgacaagta cgacctaagc aacttgcacg agatcgccag cggcggggcg ccgctcagca
     2641 aggaggtagg tgaggccgtg gccaaacgct tccacctacc aggcatccgc cagggctacg
     2701 gcctgacaga aacaaccagc gccattctga tcacccccga aggggacgac aagcctggcg
     2761 cagtaggcaa ggtggtgccc ttcttcgagg ctaaggtggt ggacttggac accggtaaga
     2821 cactgggtgt gaaccagcgc ggcgagctgt gcgtccgtgg ccccatgatc atgagcggct
     2881 acgttaacaa ccccgaggct acaaacgctc tcatcgacaa ggacggctgg ctgcacagcg
     2941 gcgacatcgc ctactgggac gaggacgagc acttcttcat cgtggaccgg ctgaagagcc
     3001 tgatcaaata caagggctac caggtagccc cagccgaact ggagagcatc ctgctgcaac
     3061 accccaacat cttcgacgcc ggggtcgccg gcctgcccga cgacgatgcc ggcgagctgc
     3121 ccgccgcagt cgtcgtgctg gaacacggta aaaccatgac cgagaaggag atcgtggact
     3181 atgtggccag ccaggttaca accgccaaga agctgcgcgg tggtgttgtg ttcgtggacg
     3241 aggtgcctaa aggactgacc ggcaagttgg acgcccgcaa gatccgcgag attctcatta
     3301 aggccaagaa gggcggcaag atcgccgtgt aataattcta gagtcggggc ggccggccgc
     3361 ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     3421 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     3481 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     3541 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atcgataagg
     3601 atcgatccgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc
     3661 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg
     3721 acaggtgccg gcagcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     3781 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     3841 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     3901 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     3961 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     4021 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     4081 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     4141 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     4201 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     4261 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     4321 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     4381 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     4441 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     4501 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     4561 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     4621 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagcg
     4681 gccgcaaatg ctaaaccact gcagtggtta ccagtgcttg atcagtgagg caccgatctc
     4741 agcgatctgc ctatttcgtt cgtccatagt ggcctgactc cccgtcgtgt agatcactac
     4801 gattcgtgag ggcttaccat caggccccag cgcagcaatg atgccgcgag agccgcgttc
     4861 accggccccc gatttgtcag caatgaacca gccagcaggg agggccgagc gaagaagtgg
     4921 tcctgctact ttgtccgcct ccatccagtc tatgagctgc tgtcgtgatg ctagagtaag
     4981 aagttcgcca gtgagtagtt tccgaagagt tgtggccatt gctactggca tcgtggtatc
     5041 acgctcgtcg ttcggtatgg cttcgttcaa ctctggttcc cagcggtcaa gccgggtcac
     5101 atgatcaccc atattatgaa gaaatgcagt cagctcctta gggcctccga tcgttgtcag
     5161 aagtaagttg gccgcggtgt tgtcgctcat ggtaatggca gcactacaca attctcttac
     5221 cgtcatgcca tccgtaagat gcttttccgt gaccggcgag tactcaacca agtcgttttg
     5281 tgagtagtgt atacggcgac caagctgctc ttgcccggcg tctatacggg acaacaccgc
     5341 gccacatagc agtactttga aagtgctcat catcgggaat cgttcttcgg ggcggaaaga
     5401 ctcaaggatc ttgccgctat tgagatccag ttcgatatag cccactcttg cacccagttg
     5461 atcttcagca tcttttactt tcaccagcgt ttcggggtgt gcaaaaacag gcaagcaaaa
     5521 tgccgcaaag aagggaatga gtgcgacacg aaaatgttgg atgctcatac tcgtcctttt
     5581 tcaatattat tgaagcattt atcagggtta ctagtacgtc tctcaaggat aagtaagtaa
     5641 tattaaggta cgggaggtat tggacaggcc gcaataaaat atctttattt tcattacatc
     5701 tgtgtgttgg ttttttgtgt gaatcgatag tactaacata cgctctccat caaaacaaaa
     5761 cgaaacaaaa caaactagca aaataggctg tccccagtgc aagtgcaggt gccagaacat
     5821 ttctctggcc taactggccg gtacctgagc tcgctagcc
//
