LOCUS       sequence-351622-        5261 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid containing the SF3B3-miniXon/Firefly luciferase cassette. 
            Translation of Firefly luciferase is controlled by splicing 
            modulation of the SF3B3-miniXon by LMI070..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Monteys AM, Hundley AA, Ranum PT, Tecedor L, Muehlmatt A, Lim E, 
            Lukashev D, Sivasankaran R, Davidson BL
  TITLE     Regulated control of gene therapies by drug-induced splicing.
  JOURNAL   Nature. 2021 Jul 28. pii: 10.1038/s41586-021-03770-2. doi: 
            10.1038/s41586-021-03770-2.
  PUBMED    34321659
REFERENCE   2  (bases 1 to 5261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 Jul 28. pii: 10.1038/s41586-021-03770-2. doi: 
            10.1038/s41586-021-03770-2."
COMMENT     Sequence Label: pGL4.10/RSV_SF3B3-miniXon_Luciferase
FEATURES             Location/Qualifiers
     source          1..5261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        196..458
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     regulatory      982..991
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1085..2734
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAH
                     IEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPA
                     NDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSM
                     YTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHAR
                     DPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKI
                     QSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG
                     LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG
                     YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL
                     QHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVF
                     VDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2778..2899
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2789..2808)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2843..2862
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3151..3168)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3322..3910)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3402..3421)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4110..4901,4902..4970))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5075..5123
                     /note="synthetic polyadenylation signal"
     misc_feature    5137..5228
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5177..5196
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 tcgagcctgc aggcgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
       61 ttcgcgatgt acgggccaga tatacgcgta tctgagggga ctagggtgtg tttaggcgaa
      121 aagcggggct tcggttgtac gcggttagga gtcccctcag gatatagtag tttcgctttt
      181 gcatagggag ggggaaatgt agtcttatgc aatacacttg tagtcttgca acatggtaac
      241 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
      301 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acaggtctga catggattgg
      361 acgaaccact gaattgcgca ttgcagagat aattgtattt aagtgcctag ctcgatacaa
      421 taaacgccat ttgaccattc accacattgg tgtgcacctc caaggccaca gatccgttta
      481 aacaagcttg gcaatccggt actgttggta aatttctgta caacttaacc ttgcagagag
      541 ccactggcat cagctttgcc attcttggaa acttttctgg taagttctct cgttaccatc
      601 ttttgaaatt ttaagtgaat taatacatat cttgcttagt ctcttgtgca ggaaatgttt
      661 tccatttatg acaaaacagg aattgtgtga aatttcaaat attggattag gaaatacaaa
      721 gttactgaaa gtgaggtact aatgtttata aaataaaaac tttttcttgc catttgcaga
      781 tttaacattt ttgagtcaat ccaagtgcca ccatgcagga ggttcatgat tgtgtagagt
      841 aagacataat tttgttgagg tttaactctg aatacttaat gtggtactga atacttaatg
      901 tggtactgag aggcagccta actgaccaca cagcattcac gcctggctaa tttttgtatt
      961 tttagtagag acggggtttc accatggtgg ccaggctggg ttctggttgt ttatgatctt
     1021 tattttttgg tgatctagga accaaacaac aagaaattgt tgtttcccgt gggtaagagg
     1081 atccgaagat gccaaaaaca ttaagaaggg cccagcgcca ttctacccac tcgaagacgg
     1141 gaccgccggc gagcagctgc acaaagccat gaagcgctac gccctggtgc ccggcaccat
     1201 cgcctttacc gacgcacata tcgaggtgga cattacctac gccgagtact tcgagatgag
     1261 cgttcggctg gcagaagcta tgaagcgcta tgggctgaat acaaaccatc ggatcgtggt
     1321 gtgcagcgag aatagcttgc agttcttcat gcccgtgttg ggtgccctgt tcatcggtgt
     1381 ggctgtggcc ccagctaacg acatctacaa cgagcgcgag ctgctgaaca gcatgggcat
     1441 cagccagccc accgtcgtat tcgtgagcaa gaaagggctg caaaagatcc tcaacgtgca
     1501 aaagaagcta ccgatcatac aaaagatcat catcatggat agcaagaccg actaccaggg
     1561 cttccaaagc atgtacacct tcgtgacttc ccatttgcca cccggcttca acgagtacga
     1621 cttcgtgccc gagagcttcg accgggacaa aaccatcgcc ctgatcatga acagtagtgg
     1681 cagtaccgga ttgcccaagg gcgtagccct accgcaccgc accgcttgtg tccgattcag
     1741 tcatgcccgc gaccccatct tcggcaacca gatcatcccc gacaccgcta tcctcagcgt
     1801 ggtgccattt caccacggct tcggcatgtt caccacgctg ggctacttga tctgcggctt
     1861 tcgggtcgtg ctcatgtacc gcttcgagga ggagctattc ttgcgcagct tgcaagacta
     1921 taagattcaa tctgccctgc tggtgcccac actatttagc ttcttcgcta agagcactct
     1981 catcgacaag tacgacctaa gcaacttgca cgagatcgcc agcggcgggg cgccgctcag
     2041 caaggaggta ggtgaggccg tggccaaacg cttccaccta ccaggcatcc gccagggcta
     2101 cggcctgaca gaaacaacca gcgccattct gatcaccccc gaaggggacg acaagcctgg
     2161 cgcagtaggc aaggtggtgc ccttcttcga ggctaaggtg gtggacttgg acaccggtaa
     2221 gacactgggt gtgaaccagc gcggcgagct gtgcgtccgt ggccccatga tcatgagcgg
     2281 ctacgttaac aaccccgagg ctacaaacgc tctcatcgac aaggacggct ggctgcacag
     2341 cggcgacatc gcctactggg acgaggacga gcacttcttc atcgtggacc ggctgaagag
     2401 cctgatcaaa tacaagggct accaggtagc cccagccgaa ctggagagca tcctgctgca
     2461 acaccccaac atcttcgacg ccggggtcgc cggcctgccc gacgacgatg ccggcgagct
     2521 gcccgccgca gtcgtcgtgc tggaacacgg taaaaccatg accgagaagg agatcgtgga
     2581 ctatgtggcc agccaggtta caaccgccaa gaagctgcgc ggtggtgttg tgttcgtgga
     2641 cgaggtgcct aaaggactga ccggcaagtt ggacgcccgc aagatccgcg agattctcat
     2701 taaggccaag aagggcggca agatcgccgt gtaataattc tagagtcggg gcggccggcc
     2761 gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     2821 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     2881 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     2941 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta aaatcgataa
     3001 ggatcgatcc gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg
     3061 gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta
     3121 ggacaggtgc cggcagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     3181 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     3241 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     3301 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     3361 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     3421 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     3481 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     3541 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     3601 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     3661 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     3721 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     3781 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     3841 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     3901 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     3961 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     4021 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     4081 cggccgcaaa tgctaaacca ctgcagtggt taccagtgct tgatcagtga ggcaccgatc
     4141 tcagcgatct gcctatttcg ttcgtccata gtggcctgac tccccgtcgt gtagatcact
     4201 acgattcgtg agggcttacc atcaggcccc agcgcagcaa tgatgccgcg agagccgcgt
     4261 tcaccggccc ccgatttgtc agcaatgaac cagccagcag ggagggccga gcgaagaagt
     4321 ggtcctgcta ctttgtccgc ctccatccag tctatgagct gctgtcgtga tgctagagta
     4381 agaagttcgc cagtgagtag tttccgaaga gttgtggcca ttgctactgg catcgtggta
     4441 tcacgctcgt cgttcggtat ggcttcgttc aactctggtt cccagcggtc aagccgggtc
     4501 acatgatcac ccatattatg aagaaatgca gtcagctcct tagggcctcc gatcgttgtc
     4561 agaagtaagt tggccgcggt gttgtcgctc atggtaatgg cagcactaca caattctctt
     4621 accgtcatgc catccgtaag atgcttttcc gtgaccggcg agtactcaac caagtcgttt
     4681 tgtgagtagt gtatacggcg accaagctgc tcttgcccgg cgtctatacg ggacaacacc
     4741 gcgccacata gcagtacttt gaaagtgctc atcatcggga atcgttcttc ggggcggaaa
     4801 gactcaagga tcttgccgct attgagatcc agttcgatat agcccactct tgcacccagt
     4861 tgatcttcag catcttttac tttcaccagc gtttcggggt gtgcaaaaac aggcaagcaa
     4921 aatgccgcaa agaagggaat gagtgcgaca cgaaaatgtt ggatgctcat actcgtcctt
     4981 tttcaatatt attgaagcat ttatcagggt tactagtacg tctctcaagg ataagtaagt
     5041 aatattaagg tacgggaggt attggacagg ccgcaataaa atatctttat tttcattaca
     5101 tctgtgtgtt ggttttttgt gtgaatcgat agtactaaca tacgctctcc atcaaaacaa
     5161 aacgaaacaa aacaaactag caaaataggc tgtccccagt gcaagtgcag gtgccagaac
     5221 atttctctgg cctaactggc cggtacctga gctcgctagc c
//
