LOCUS       sequence-351623-       10437 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination vector to insert genes of interest having a 
            C-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10437)
  AUTHORS   Evans-Yamamoto D, Rouleau FD, Nanda P, Makanae K, Liu Y, Despres PC,
            Matsuo H, Seki M, Dube AK, Ascencio D, Yachie N, Landry CR
  TITLE     Barcode fusion genetics-protein-fragment complementation assay 
            (BFG-PCA): tools and resources that expand the potential for binary 
            protein interaction discovery.
  JOURNAL   Nucleic Acids Res. 2022 Feb 7. pii: 6523796. doi: 
            10.1093/nar/gkac045.
  PUBMED    35137167
REFERENCE   2  (bases 1 to 10437)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2022 Feb 7. pii: 6523796. doi: 10.1093/nar/gkac045."
COMMENT     Sequence Label: pDEST-DHFR F[1,2]-C (TRP1)
FEATURES             Location/Qualifiers
     source          1..10437
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        353..457
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(458..526,527..1318)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(676..695)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1489..2077
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1978..1997
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2231..2248
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2365..2386
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2401..2406,2407..2424,2425..2431)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2439..2455
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2444..2466
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2463..2479
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2463..2479
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2500..2518
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      3733..3980
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(3749..3767)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     protein_bind    5506..5630
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        5804..5906
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             5907..6587
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(5973..5992)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             6907..7212
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     7091..7110
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(7253..7377)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      7956..8143
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(8011..8035)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(8295..8314)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8296..8314)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8321..8338)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8321..8337)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8330..8352)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8478..8933
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8565..8584)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8775..8796
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(9036..9710)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(9711..9991)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(10012..10031)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10131..10153
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10191..10209)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    join(10250..10437,1..316)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 actttacata tatatttatt agacaagaaa agcagattaa atagatatac attcgattaa
       61 cgataagtaa aatgtaaaat cacaggattt tcgtgtgtgg tcttctacac agacaagatg
      121 aaacaattcg gcattaatac ctgagagcag gaagagcaag ataaaaggta gtatttgttg
      181 gcgatccccc tagagtcttt tacatcttcg gaaaacaaaa actatttttt ctttaatttc
      241 tttttttact ttctattttt aatttatata tttatattaa aaaatttaaa ttataattat
      301 ttttatagca cgtgatgaaa aggacccagg tggcactttt cggggaaatg tgcgcggaac
      361 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
      421 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
      481 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
      541 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
      601 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
      661 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
      721 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
      781 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
      841 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
      901 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
      961 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     1021 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     1081 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     1141 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     1201 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     1261 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     1321 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     1381 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     1441 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1501 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1561 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1621 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     1681 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     1741 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     1801 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     1861 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     1921 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     1981 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     2041 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     2101 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     2161 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     2221 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     2281 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     2341 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     2401 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     2461 cacaggaaac agctatgacc atgattacgc caagctcgaa attaaccctc actaaaggga
     2521 acaaaagctg ggatccccag attctatttg cagttgccaa tcctgtatct cttgtgcggc
     2581 tccatcaaaa gtcacgccac gtagcttaca tagagataac ttagcatcgg tgactcttcc
     2641 atgtgctaac aaccattttg gagattcatc gaaaaacaca gcaaaaacaa gagtactgta
     2701 accaatgtaa catctgtaca ccagggaccc acacattacc aaaatcaaaa ttatttttct
     2761 aatgcctgtt atttttccta tttttcctct ggcgcgtgaa tagcccgcag agacgcaaac
     2821 aattttcctc gcagtttttc gcttgtttaa tgcgtatttt cccagatagg ttcaaacctt
     2881 tcatctgtat cccgtatatt taagatggcg tttgctttct ccgttgattt ttttccttct
     2941 tagtgatttt tttgcattaa atcccagaac aatcatccaa ctaatcaaga atgccttcca
     3001 gattcactaa gactagaaag cacagaggtc acgtctcagc cggtaaaggt cgtatcggta
     3061 agcacagaaa gcaccccggt ggtagaggta tggccggtgg tcaacatcac cacagaatta
     3121 acatggataa ataccatcca ggttatttcg gtaaggttgg tatgagatac ttccacaagc
     3181 aacaagctca tttctggaag ccagtcttga acttggacaa attgtggaca ttgatcccag
     3241 aagacaagag agaccaatac ttgaaatctg cttctaagga aactgctcca gttattgaca
     3301 ctttggcagc cggttacggt aagatcttgg gtaagggtag aattccaaat gttccagtta
     3361 tcgtcaaagc tagattcgtc tccaagttgg ctgaagaaaa aatcagagct gctggtggtg
     3421 ttgttgaatt gatcgcttaa gcgcatcaac aaaaactcta tgtattttcc aataaattat
     3481 atatcttcag tttaatctaa ttcaacatct acttctgtat tatttctatc aaaaaaaaaa
     3541 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     3601 aaaaaaaaaa aaaaaaaaaa aaaagtacca agatggcctt tggtgggttg aagaaggaaa
     3661 aagacagaaa cgacttaatt acctacttga aaaaagcctg tgagtaaaca ggcccctttt
     3721 cctttgtcga tatcatgtaa ttagttatgt cacgcttaca ttcacgccct ccccccacat
     3781 ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct atttattttt
     3841 ttatagttat gttagtatta agaacgttat ttatatttca aatttttctt ttttttctgt
     3901 acagacgcgt gtacgcatgt aacattatac tgaaaacctt gcttgagaag gttttgggac
     3961 gctcgaaggc tttaatttgc aagctcggga tccgggcccc ccctcgagat ccgggatcga
     4021 agaaatgatg gtaaatgaaa taggaaatca aggagcatga aggcaaaaga caaatataag
     4081 ggtcgaacga aaaataaagt gaaaagtgtt gatatgatgt atttggcttt gcggcgccga
     4141 aaaaacgagt ttacgcaatt gcacaatcat gctgactctg tggcggaccc gcgctcttgc
     4201 cggcccggcg ataacgctgg gcgtgaggct gtgcccggcg gagttttttg cgcctgcatt
     4261 ttccaaggtt taccctgcgc taaggggcga gattggagaa gcaataagaa tgccggttgg
     4321 ggttgcgatg atgacgacca cgacaactgg tgtcattatt taagttgccg aaagaacctg
     4381 agtgcatttg caacatgagt atactagaag aatgagccaa gacttgcgag acgcgagttt
     4441 gccggtggtg cgaacaatag agcgaccatg accttgaagg tgagacgcgc ataaccgcta
     4501 gagtactttg aagaggaaac agcaataggg ttgctaccag tataaataga caggtacata
     4561 caacactgga aatggttgtc tgtttgagta cgctttcaat tcatttgggt gtgcacttta
     4621 ttatgttaca atatggaagg gaactttaca cttctcctat gcacatatat taatgtaact
     4681 ataacggtcc taaggtagcg aaattaaagt ccaatgctag tagagaaggg gggtaacacc
     4741 cctccgcgct cttttccgat ttttttctaa accgtggaat atttcggata tccttttgtt
     4801 gtttccgggt gtacaatatg gacttcctct tttctggcaa ccaaacccat acatcgggat
     4861 tcctataata ccttcgttgg tctccctaac atgtaggtgg cggaggggag atatacaata
     4921 gaacagatac cagacaagac ataatgggct aaacaagact acaccaatta cactgcctca
     4981 ttgatggtgg tacataacga actaatactg tagccctaga cttgatagcc atcatcatat
     5041 cgaagtttca ctaccctttt tccatttgcc atctattgaa gtaataatag gcgcatgcaa
     5101 cttcttttct ttttttttct tttctctctc ccccgttgtt gtctcaccat atccgcaatg
     5161 acaaaaaaat gatggaagac actaaaggaa aaaattaacg acaaagacag caccaacaga
     5221 tgtcgttgtt ccagagctga tgaggggtat ctcgaagcac acgaaacttt ttccttcctt
     5281 cattcacgca cactactctc taatgagcaa cggtatacgg ccttccttcc agttacttga
     5341 atttgaaata aaaaaaagtt tgctgtcttg ctatcaagta taaatagacc tgcaattatt
     5401 aatcttttgt ttcctcgtca ttgttctcgt tccctttctt ccttgtttct ttttctgcac
     5461 aatatttcaa gctataccaa gcatacaaag ggtgggtcga atcaaacaag tttgtacaaa
     5521 aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat
     5581 aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg gcggccgcgg
     5641 gtgatgctgc caacttagcg gccgctaagt tggcagcatc acccgacgca ctttgcgccg
     5701 aataaatacc tgtgacggaa gatcacttcg cagaataaat aaatcctggt gtccctgttg
     5761 ataccgggaa gccctgggcc aacttttggc gaaaatgaga cgttgatcgg cacgtaagag
     5821 gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt gagtcatcga
     5881 gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg
     5941 atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta
     6001 cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata
     6061 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg
     6121 aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt
     6181 acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg
     6241 atttccggca gtttctacat atatattcgc aagatgtggc gtgttacggt gaaaacctgg
     6301 cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga
     6361 gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca
     6421 ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc
     6481 atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact
     6541 gcgatgagtg gcaggcgggg cgtaatctag aggatccggc ttactaaaag ccagataaca
     6601 gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg
     6661 aagtatgtca aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac
     6721 agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca
     6781 tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga
     6841 tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct
     6901 ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg
     6961 gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt
     7021 gcacgtctgc tgtcagataa agtcccccgt gaactttacc cggtggtgca tatcggggat
     7081 gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa
     7141 gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc
     7201 tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga
     7261 ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa
     7321 tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtagg
     7381 aggaggaagt actagtatgg ttcgaccatt gaactgcatc gtcgccgtgt cccaaaatat
     7441 ggggattggc aagaacggag actatccctg gcctccgctc aggaacgagt ccaagtactt
     7501 ccaaagaatg accacaacct cttcagtgga aggtaaacag aatctggtga ttatgggtag
     7561 gaaaacctgg ttctccattc ctgagaagaa tcgaccttta aaggacagaa ttaatatagt
     7621 tctcagtaga gaactcaaag aaccaccacg aggagctcat tttcttgcca aaagtttgga
     7681 tgatgcctta agacttattg aacaaccgga attgggtacc taatttaagt aagtaatggc
     7741 aaacagctat tatgggtatt atgggttagg gataacaggg taataagctc taagtaagta
     7801 acggccgcca ccgcggtgga gctttggact tcttcgccag aggtttggtc aagtctccaa
     7861 tcaaggttgt cggcttgtct accttgccag aaatttacga aaagatggaa aagggtcaaa
     7921 tcgttggtag atacgttgtt gacacttcta aataagcgaa tttcttatga tttatgattt
     7981 ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg
     8041 ttttaaaacg aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc
     8101 aggtatagca tgaggtcgct cttattgacc acacctctac cggcatgccg agcaaatgcc
     8161 tgcaaatcgc tccccatttc acccaattgt agatatgcta actccagcaa tgagttgatg
     8221 aatctcggtg tgtattttat gtcctcagag gacaacacct gttgtaatcg ttcttccaca
     8281 cggatcccaa ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac
     8341 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     8401 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     8461 gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     8521 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     8581 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     8641 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     8701 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     8761 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     8821 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     8881 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttcctgatg
     8941 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagatcg gcaagtgcac
     9001 aaacaatact taaataaata ctactcagta ataacctatt tcttagcatt tttgacgaaa
     9061 tttgctattt tgttagagtc ttttacacca tttgtctcca cacctccgct tacatcaaca
     9121 ccaataacgc catttaatct aagcgcatca ccaacatttt ctggcgtcag tccaccagct
     9181 aacataaaat gtaagctttc ggggctctct tgccttccaa cccagtcaga aatcgagttc
     9241 caatccaaaa gttcacctgt cccacctgct tctgaatcaa acaagggaat aaacgaatga
     9301 ggtttctgtg aagctgcact gagtagtatg ttgcagtctt ttggaaatac gagtctttta
     9361 ataactggca aaccgaggaa ctcttggtat tcttgccacg actcatctcc atgcagttgg
     9421 acgatatcaa tgccgtaatc attgaccaga gccaaaacat cctccttagg ttgattacga
     9481 aacacgccaa ccaagtattt cggagtgcct gaactatttt tatatgcttt tacaagactt
     9541 gaaattttcc ttgcaataac cgggtcaatt gttctctttc tattgggcac acatataata
     9601 cccagcaagt cagcatcgga atctagagca cattctgcgg cctctgtgct ctgcaagccg
     9661 caaactttca ccaatggacc agaactacct gtgaaattaa taacagacat actccaagct
     9721 gcctttgtgt gcttaatcac gtatactcac gtgctcaata gtcaccaatg ccctccctct
     9781 tggccctctc cttttctttt ttcgaccgaa ttaattctta atcggcaaaa aaagaaaagc
     9841 tccggatcaa gattgtacgt aaggtgacaa gctatttttc aataaagaat atcttccact
     9901 actgccatct ggcgtcataa ctgcaaagta cacatatatt acgatgctgt tctattaaat
     9961 gcttcctata ttatatatat agtaatgtcg tgatctatgg tgcactctca gtacaatctg
    10021 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
    10081 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
    10141 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
    10201 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgg atcgcttgcc
    10261 tgtaacttac acgcgcctcg tatcttttaa tgatggaata atttgggaat ttactctgtg
    10321 tttatttatt tttatgtttt gtatttggat tttagaaagt aaataaagaa ggtagaagag
    10381 ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa atttcaacaa aaagcgt
//
