LOCUS       sequence-35165-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Levy C, Khaled M, Iliopoulos D, Janas MM, Schubert S, Pinner S, Chen
            PH, Li S, Fletcher AL, Yokoyama S, Scott KL, Garraway LA, Song JS, 
            Granter SR, Turley SJ, Fisher DE, Novina CD
  TITLE     Intronic miR-211 assumes the tumor suppressive function of its host 
            gene in melanoma.
  JOURNAL   Mol Cell. 2010 Dec 10. 40(5):841-9.
  PUBMED    21109473
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2010 Dec 10. 40(5):841-9."
COMMENT     Sequence Label: PLKO-mcherry-luc-puro-Renilla cDNA
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    155..272
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        321..831
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     750..768
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      840..849
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atactgaatt tgtcaaagta aaaggtcttc atttttcgca agaagatgca cctgatgaaa
       61 tgggaaaata tatcaaatcg ttcgttgagc gagttctcaa aaatgaacaa taagaattct
      121 cgacctcgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
      181 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
      241 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
      301 cactttggcc gcggctcgag ggggttgggg ttgcgccttt tccaaggcag ccctgggttt
      361 gcgcagggac gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg
      421 tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg
      481 ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg
      541 gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc agacggacag
      601 cgccagggag caatggcagc gcgccgaccg cgatgggctg tggccaatag cggctgctca
      661 gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg
      721 gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc
      781 acgtcggcag tcggctccct cgttgaccga atcaccgacc tctctcccca gggggatccg
      841 ccaccatgga agatgccaag aacatca
//
