LOCUS       sequence-351688-       10111 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination vector to insert genes of interest having a 
            N-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10111)
  AUTHORS   Evans-Yamamoto D, Rouleau FD, Nanda P, Makanae K, Liu Y, Despres PC,
            Matsuo H, Seki M, Dube AK, Ascencio D, Yachie N, Landry CR
  TITLE     Barcode fusion genetics-protein-fragment complementation assay 
            (BFG-PCA): tools and resources that expand the potential for binary 
            protein interaction discovery.
  JOURNAL   Nucleic Acids Res. 2022 Feb 7. pii: 6523796. doi: 
            10.1093/nar/gkac045.
  PUBMED    35137167
REFERENCE   2  (bases 1 to 10111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2022 Feb 7. pii: 6523796. doi: 10.1093/nar/gkac045."
COMMENT     Sequence Label: pDEST-DHFR F[3]-N (LEU2)
FEATURES             Location/Qualifiers
     source          1..10111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..23
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     20..36
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     20..36
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        57..75
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        889..1593
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     protein_bind    1865..1989
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2136..2238
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2239..2919
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(2305..2324)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3239..3544
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3423..3442
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3585..3709)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3968..4155
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4023..4047)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(4307..4326)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4308..4326)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4333..4350)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4333..4349)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4342..4364)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4490..4945
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4577..4596)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4787..4808
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5246..5653
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             5654..6748
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(7242..7261)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7361..7383
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7421..7439)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    7481..7984
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        8021..8125
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8126..8194,8195..8986)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8344..8363)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9157..9745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9646..9665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9899..9916
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10033..10054
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10069..10074,10075..10092,10093..10099)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    join(10107..10111,1..12)
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctcgaaatt
       61 aaccctcact aaagggaaca aaagctggta ccgggccccc cctcgagatc cgggatcgaa
      121 gaaatgatgg taaatgaaat aggaaatcaa ggagcatgaa ggcaaaagac aaatataagg
      181 gtcgaacgaa aaataaagtg aaaagtgttg atatgatgta tttggctttg cggcgccgaa
      241 aaaacgagtt tacgcaattg cacaatcatg ctgactctgt ggcggacccg cgctcttgcc
      301 ggcccggcga taacgctggg cgtgaggctg tgcccggcgg agttttttgc gcctgcattt
      361 tccaaggttt accctgcgct aaggggcgag attggagaag caataagaat gccggttggg
      421 gttgcgatga tgacgaccac gacaactggt gtcattattt aagttgccga aagaacctga
      481 gtgcatttgc aacatgagta tactagaaga atgagccaag acttgcgaga cgcgagtttg
      541 ccggtggtgc gaacaataga gcgaccatga ccttgaaggt gagacgcgca taaccgctag
      601 agtactttga agaggaaaca gcaatagggt tgctaccagt ataaatagac aggtacatac
      661 aacactggaa atggttgtct gtttgagtac gctttcaatt catttgggtg tgcactttat
      721 tatgttacaa tatggaaggg aactttacac ttctcctatg cacatatatt aatgtaacta
      781 taacggtcct aaggtagcga aattaaagtc caatgctagt agagaagggg ggtaacaccc
      841 ctccgcgctc ttttccgatt tttttctaaa ccgtggaata tttcggatat ccttttgttg
      901 tttccgggtg tacaatatgg acttcctctt ttctggcaac caaacccata catcgggatt
      961 cctataatac cttcgttggt ctccctaaca tgtaggtggc ggaggggaga tatacaatag
     1021 aacagatacc agacaagaca taatgggcta aacaagacta caccaattac actgcctcat
     1081 tgatggtggt acataacgaa ctaatactgt agccctagac ttgatagcca tcatcatatc
     1141 gaagtttcac tacccttttt ccatttgcca tctattgaag taataatagg cgcatgcaac
     1201 ttcttttctt tttttttctt ttctctctcc cccgttgttg tctcaccata tccgcaatga
     1261 caaaaaaatg atggaagaca ctaaaggaaa aaattaacga caaagacagc accaacagat
     1321 gtcgttgttc cagagctgat gaggggtatc tcgaagcaca cgaaactttt tccttccttc
     1381 attcacgcac actactctct aatgagcaac ggtatacggc cttccttcca gttacttgaa
     1441 tttgaaataa aaaaaagttt gctgtcttgc tatcaagtat aaatagacct gcaattatta
     1501 atcttttgtt tcctcgtcat tgttctcgtt ccctttcttc cttgtttctt tttctgcaca
     1561 atatttcaag ctataccaag catacaatca actccaagct tggatgagta aagtagacat
     1621 ggtttggata gtcggaggca gttctgttta ccaggaagcc atgaatcaac caggccacct
     1681 cagactcttt gtgacaagga tcatgcagga atttgaaagt gacacgtttt tcccagaaat
     1741 tgatttgggg aaatataaac ttctcccaga atacccaggc gtcctctctg aggtccagga
     1801 ggaaaaaggc atcaagtata agtttgaagt ctacgagaag aaagacggag gaggaagtac
     1861 tagtacaagt ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat
     1921 attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca
     1981 gtcactatgg cggccgctaa gttggcagca tcacccgacg cactttgcgc cgaataaata
     2041 cctgtgacgg aagatcactt cgcagaataa ataaatcctg gtgtccctgt tgataccggg
     2101 aagccctggg ccaacttttg gcgaaaatga gacgttgatc ggcacgtaag aggttccaac
     2161 tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagtcatc gagattttca
     2221 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
     2281 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
     2341 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
     2401 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt
     2461 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
     2521 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
     2581 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
     2641 cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc
     2701 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc
     2761 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc
     2821 gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag
     2881 tggcaggcgg ggcgtaatct agaggatccg gcttactaaa agccagataa cagtatgcgt
     2941 atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc cgaagtatgt
     3001 caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg acagctatca
     3061 gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac catgcagaat
     3121 gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg gatggctgag
     3181 gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg ctggtgaaat
     3241 gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca
     3301 gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca gtgcacgtct
     3361 gctgtcagat aaagtccccc gtgaacttta cccggtggtg catatcgggg atgaaagctg
     3421 gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg aagaagtggc
     3481 tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat
     3541 ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt gactggatat
     3601 gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga
     3661 tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt tgatggccgc
     3721 taagtaagta agagctttaa gtaagtaatg gcaaacagct attatgggta ttatgggtta
     3781 gggataacag ggtaataagc tctaagtaag taacggccgc caccgcggtg gagctttgga
     3841 cttcttcgcc agaggtttgg tcaagtctcc aatcaaggtt gtcggcttgt ctaccttgcc
     3901 agaaatttac gaaaagatgg aaaagggtca aatcgttggt agatacgttg ttgacacttc
     3961 taaataagcg aatttcttat gatttatgat ttttattatt aaataagtta taaaaaaaat
     4021 aagtgtatac aaattttaaa gtgactctta ggttttaaaa cgaaaattct tattcttgag
     4081 taactctttc ctgtaggtca ggttgctttc tcaggtatag catgaggtcg ctcttattga
     4141 ccacacctct accggcatgc cgagcaaatg cctgcaaatc gctccccatt tcacccaatt
     4201 gtagatatgc taactccagc aatgagttga tgaatctcgg tgtgtatttt atgtcctcag
     4261 aggacaacac ctgttgtaat cgttcttcca cacggatccc aattcgccct atagtgagtc
     4321 gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     4381 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     4441 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt
     4501 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4561 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4621 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4681 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     4741 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4801 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     4861 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     4921 aacaaaatat taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg
     4981 gtatttcaca ccgcatatcg accggtcgag gagaacttct agtatatcta catacctaat
     5041 attattgcct tattaaaaat ggaatcccaa caattacatc aaaatccaca ttctcttcaa
     5101 aatcaattgt cctgtacttc cttgttcatg tgtgttcaaa aacgttatat ttataggata
     5161 attatactct atttctcaac aagtaattgg ttgtttggcc gagcggtcta aggcgcctga
     5221 ttcaagaaat atcttgaccg cagttaactg tgggaatact caggtatcgt aagatgcaag
     5281 agttcgaatc tcttagcaac cattattttt ttcctcaaca taacgagaac acacaggggc
     5341 gctatcgcac agaatcaaat tcgatgactg gaaatttttt gttaatttca gaggtcgcct
     5401 gacgcatata cctttttcaa ctgaaaaatt gggagaaaaa ggaaaggtga gagcgccgga
     5461 accggctttt catatagaat agagaagcgt tcatgactaa atgcttgcat cacaatactt
     5521 gaagttgaca atattattta aggacctatt gttttttcca ataggtggtt agcaatcgtc
     5581 ttactttcta acttttctta ccttttacat ttcagcaata tatatatata tatttcaagg
     5641 atataccatt ctaatgtctg cccctaagaa gatcgtcgtt ttgccaggtg accacgttgg
     5701 tcaagaaatc acagccgaag ccattaaggt tcttaaagct atttctgatg ttcgttccaa
     5761 tgtcaagttc gatttcgaaa atcatttaat tggtggtgct gctatcgatg ctacaggtgt
     5821 tccacttcca gatgaggcgc tggaagcctc caagaaggct gatgccgttt tgttaggtgc
     5881 tgtgggtggt cctaaatggg gtaccggtag tgttagacct gaacaaggtt tactaaaaat
     5941 ccgtaaagaa cttcaattgt acgccaactt aagaccatgt aactttgcat ccgactctct
     6001 tttagactta tctccaatca agccacaatt tgctaaaggt actgacttcg ttgttgtcag
     6061 agaattagtg ggaggtattt actttggtaa gagaaaggaa gacgatggtg atggtgtcgc
     6121 ttgggatagt gaacaataca ccgttccaga agtgcaaaga atcacaagaa tggccgcttt
     6181 catggcccta caacatgagc caccattgcc tatttggtcc ttggataaag ctaatgtttt
     6241 ggcctcttca agattatgga gaaaaactgt ggaggaaacc atcaagaacg aattccctac
     6301 attgaaggtt caacatcaat tgattgattc tgccgccatg atcctagtta agaacccaac
     6361 ccacctaaat ggtattataa tcaccagcaa catgtttggt gatatcatct ccgatgaagc
     6421 ctccgttatc ccaggttcct tgggtttgtt gccatctgcg tccttggcct ctttgccaga
     6481 caagaacacc gcatttggtt tgtacgaacc atgccacggt tctgctccag atttgccaaa
     6541 gaataaggtc aaccctatcg ccactatctt gtctgctgca atgatgttga aattgtcatt
     6601 gaacttgcct gaagaaggta aggccattga agatgcagtt aaaaaggttt tggatgcagg
     6661 tatcagaact ggtgatttag gtggttccaa cagtaccacc gaagtcggtg atgctgtcgc
     6721 cgaagaagtt aagaaaatcc ttgcttaaaa agattctctt tttttatgat atttgtacat
     6781 aaactttata aatgaaattc ataatagaaa cgacacgaaa ttacaaaatg gaatatgttc
     6841 atagggtaga cgaaactata tacgcaatct acatacattt atcaagaagg agaaaaagga
     6901 ggatgtaaag gaatacaggt aagcaaattg atactaatgg ctcaacgtga taaggaaaaa
     6961 gaattgcact ttaacattaa tattgacaag gaggagggca ccacacaaaa agttaggtgt
     7021 aacagaaaat catgaaacta tgattcctaa tttatatatt ggaggatttt ctctaaaaaa
     7081 aaaaaaatac aacaaataaa aaacactcaa tgacctgacc atttgatgga gtttaagtca
     7141 ataccttctt gaaccatttc ccataatggt gaaagttccc tcaagaattt tactctgtca
     7201 gaaacggcct taacgacgta gtcgatatgg tgcactctca gtacaatctg ctctgatgcc
     7261 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     7321 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     7381 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     7441 ttataggtta atgtcatgat aataatggtt tcttaggacg gatcgcttgc ctgtaactta
     7501 cacgcgcctc gtatctttta atgatggaat aatttgggaa tttactctgt gtttatttat
     7561 ttttatgttt tgtatttgga ttttagaaag taaataaaga aggtagaaga gttacggaat
     7621 gaagaaaaaa aaataaacaa aggtttaaaa aatttcaaca aaaagcgtac tttacatata
     7681 tatttattag acaagaaaag cagattaaat agatatacat tcgattaacg ataagtaaaa
     7741 tgtaaaatca caggattttc gtgtgtggtc ttctacacag acaagatgaa acaattcggc
     7801 attaatacct gagagcagga agagcaagat aaaaggtagt atttgttggc gatcccccta
     7861 gagtctttta catcttcgga aaacaaaaac tattttttct ttaatttctt tttttacttt
     7921 ctatttttaa tttatatatt tatattaaaa aatttaaatt ataattattt ttatagcacg
     7981 tgatgaaaag gacccaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     8041 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     8101 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     8161 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     8221 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     8281 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     8341 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     8401 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     8461 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     8521 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     8581 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     8641 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     8701 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     8761 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     8821 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     8881 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     8941 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     9001 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     9061 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     9121 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     9181 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     9241 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     9301 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     9361 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     9421 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     9481 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     9541 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     9601 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     9661 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     9721 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     9781 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     9841 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     9901 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     9961 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
    10021 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
    10081 gcttccggct cgtatgttgt gtggaattgt g
//
