LOCUS       sequence-351698-       11735 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express YTH domain fragment of YTHDC1 with mCherry and CRY2 tag for 
            Optodroplet Assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11735)
  AUTHORS   Lee JH, Wang R, Xiong F, Krakowiak J, Liao Z, Nguyen PT, Moroz-Omori
            EV, Shao J, Zhu X, Bolt MJ, Wu H, Singh PK, Bi M, Shi CJ, Jamal N, 
            Li G, Mistry R, Jung SY, Tsai KL, Ferreon JC, Stossi F, Caflisch A, 
            Liu Z, Mancini MA, Li W
  TITLE     Enhancer RNA m6A methylation facilitates transcriptional condensate 
            formation and gene activation.
  JOURNAL   Mol Cell. 2021 Aug 19;81(16):3368-3385.e9. doi: 
            10.1016/j.molcel.2021.07.024. Epub 2021 Aug 9.
  PUBMED    34375583
REFERENCE   2  (bases 1 to 11735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.07.024"; journalName: "Mol Cell"; date: 
            "2021-08-19- 19"; volume: "81"; issue: "16"; pages: "3368-3385.e9"
COMMENT     Sequence Label: pHR-YTH-mCherry-Cry2
FEATURES             Location/Qualifiers
     source          1..11735
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    43..160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        313..720
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             1947..3440
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     misc_feature    3592..4180
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3645..3665)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4063..4074)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4183..4199)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4184..4200)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4290..4523
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4548..4566)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4549..4566)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4856..4875)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5089..5108)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5208..5230
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5268..5286)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5354..5458
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5459..5527,5528..6319)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRILYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5677..5696)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6490..7078
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6979..6998
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7232..7249
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7319..7339)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7324..7653
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7504..7639
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8787..8852
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8982..9002
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9427..9561
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9464..9483)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9518..9537
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9729..10362
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10409..10534
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11031..11264
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11449..11493
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg
       61 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      121 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
      181 cagagatcca gtttggatcg ataagcttga tatcgaattc ctgcagcccc gataaaataa
      241 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
      301 aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag
      361 aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg
      421 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
      481 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta
      541 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
      601 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
      661 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg
      721 ggggatctgg agctctcgag aattctcacg cgtcaagtgg agcaaggcag gtggacagtg
      781 gatccggagc taccatgatg tatgtgcttc aagatgcaag atttttcctc ataaagagta
      841 acaaccatga gaatgtgtct cttgccaaag cgaagggtgt atggtccacg ctccctgtaa
      901 atgagaagaa attaaatctt gcatttagat ctgcaaggag tgttatctta atattttctg
      961 tcagagagag tggaaaattt caagggtttg caagactttc ttcagaatca catcacggag
     1021 gatctcctat acactgggtg cttccagcag gaatgagtgc taaaatgctg ggaggtgtct
     1081 ttaaaattga ctggatttgc aggcgtgaat tacccttcac taagtcggct catctcacca
     1141 atccttggaa tgaacataaa ccagtaaaga tcggacgtga tggacaggaa attgaacttg
     1201 aatgtggaac ccagctttgt cttctgatgg tgtctaaagg cgaggaggat aacatggcta
     1261 taatcaagga atttatgagg ttcaaggtgc atatggaggg atctgtgaac ggtcacgagt
     1321 tcgaaatcga gggcgaaggc gaggggcgac cctatgaagg gacacagacg gccaaactga
     1381 aggtgaccaa gggtggcccc ctgcctttcg cctgggacat cctgtcaccc cagtttatgt
     1441 acggctctaa ggcttacgtt aagcaccctg ccgatattcc ggactacttg aaactgagct
     1501 tccctgaagg gttcaagtgg gaacgggtaa tgaattttga ggatggcggt gtagtcacag
     1561 ttacccagga cagctctctt caggacggag aatttatcta taaggttaaa cttcggggca
     1621 ctaacttccc atcagacggc cccgtgatgc agaagaaaac tatgggctgg gaagccagct
     1681 cagaacgcat gtatcccgag gacggagcac tgaagggaga aatcaagcag cgactcaagc
     1741 tgaaggatgg gggacattat gatgctgagg tcaaaaccac ctataaggcc aagaaacccg
     1801 ttcagcttcc tggggcatac aacgtgaata tcaaactgga tattacatcc cataacgaag
     1861 actataccat cgtggaacag tacgagcggg ccgagggcag acatagcaca gggggcatgg
     1921 atgaattgta caaggcggcc acgcgtatga agatggacaa aaagactata gtttggttta
     1981 gaagagacct aaggattgag gataatcctg cattagcagc agctgctcac gaaggatctg
     2041 tttttcctgt cttcatttgg tgtcctgaag aagaaggaca gttttatcct ggaagagctt
     2101 caagatggtg gatgaaacaa tcacttgctc acttatctca atccttgaag gctcttggat
     2161 ctgacctcac tttaatcaaa acccacaaca cgatttcagc gatcttggat tgtatccgcg
     2221 ttaccggtgc tacaaaagtc gtctttaacc acctctatga tcctgtttcg ttagttcggg
     2281 accataccgt aaaggagaag ctggtggaac gtgggatctc tgtgcaaagc tacaatggag
     2341 atctattgta tgaaccgtgg gagatatact gcgaaaaggg caaacctttt acgagtttca
     2401 attcttactg gaagaaatgc ttagatatgt cgattgaatc cgttatgctt cctcctcctt
     2461 ggcggttgat gccaataact gcagcggctg aagcgatttg ggcgtgttcg attgaagaac
     2521 tagggctgga gaatgaggcc gagaaaccga gcaatgcgtt gttaactaga gcttggtctc
     2581 caggatggag caatgctgat aagttactaa atgagttcat cgagaagcag ttgatagatt
     2641 atgcaaagaa cagcaagaaa gttgttggga attctacttc actactttct ccgtatctcc
     2701 atttcgggga aataagcgtc agacacgttt tccagtgtgc ccggatgaaa caaattatat
     2761 gggcaagaga taagaacagt gaaggagaag aaagtgcaga tctttttctt aggggaatcg
     2821 gtttaagaga gtattctcgg tatatatgtt tcaacttccc gtttactcac gagcaatcgt
     2881 tgttgagtca tcttcggttt ttcccttggg atgctgatgt tgataagttc aaggcctgga
     2941 gacaaggcag gaccggttat ccgttggtgg atgccggaat gagagagctt tgggctaccg
     3001 gatggatgca taacagaata agagtgattg tttcaagctt tgctgtgaag tttcttctcc
     3061 ttccatggaa atggggaatg aagtatttct gggatacact tttggatgct gatttggaat
     3121 gtgacatcct tggctggcag tatatctctg ggagtatccc cgatggccac gagcttgatc
     3181 gcttggacaa tcccgcgtta caaggcgcca aatatgaccc agaaggtgag tacataaggc
     3241 aatggcttcc cgagcttgcg agattgccaa ctgaatggat ccatcatcca tgggacgctc
     3301 ctttaaccgt actcaaagct tctggtgtgg aactcggaac aaactatgcg aaacccattg
     3361 tagacatcga cacagctcgt gagctactag ctaaagctat ttcaagaacc cgtgaagcac
     3421 agatcatgat cggagcagca gcccgggatc caccggtcgc caccggttca gggagcggat
     3481 ccggctcagg gagtgcggcc gcaactccca cctgcaacat gcgtgactga ctgaggccgc
     3541 gactctagag tcgacctgca ggcatgcaag cttgatatca agcttatcga taatcaacct
     3601 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     3661 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     3721 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     3781 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     3841 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     3901 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     3961 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     4021 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     4081 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     4141 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
     4201 gggaattaat tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct
     4261 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     4321 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     4381 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     4441 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     4501 gtcagtgtgg aaaatctcta gcagcatcta gaattaattc cgtgtattct atagtgtcac
     4561 ctaaatcgta tgtgtatgat acataaggtt atgtattaat tgtagccgcg ttctaacgac
     4621 aatatgtaca agcctaattg tgtagcatct ggcttactga agcagaccct atcatctctc
     4681 tcgtaaactg ccgtcagagt cggtttggtt ggacgaacct tctgagtttc tggtaacgcc
     4741 gtcccgcacc cggaaatggt cagcgaacca atcagcaggg tcatcgctag ccagatcctc
     4801 tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat
     4861 atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt
     4921 ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg
     4981 catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc
     5041 ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc actctcagta caatctgctc
     5101 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     5161 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     5221 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     5281 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     5341 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     5401 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     5461 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     5521 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     5581 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     5641 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     5701 tattgacgcc gggcaagagc aactcggtcg ccgcatactc tattctcaga atgacttggt
     5761 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     5821 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     5881 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     5941 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     6001 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     6061 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     6121 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     6181 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     6241 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     6301 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     6361 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     6421 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6481 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6541 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6601 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     6661 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6721 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6781 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6841 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6901 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6961 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     7021 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     7081 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     7141 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     7201 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     7261 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctgtgg
     7321 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     7381 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     7441 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     7501 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     7561 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     7621 ggaggctttt ttggaggcct aggcttttgc aaaaagcttg gacacaagac aggcttgcga
     7681 gatatgtttg agaataccac tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc
     7741 ggactcatgt gaaatactgg tttttagtgc gccagatctc tataatctcg cgcaacctat
     7801 tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc acatgagcga
     7861 aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg caagccgact
     7921 gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc tgtaccgggt
     7981 gcgttactgg cgcgtgaact gggtattcgt catgtcgata ccgtttgtat ttccagctac
     8041 gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc
     8101 ttcatcgtta ttgatgacct ggtggatacc ggtggtactg cggttgcgat tcgtgaaatg
     8161 tatccaaaag cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat
     8221 gactatgttg ttgatatccc gcaagatacc tggattgaac agccgtggga tatgggcgtc
     8281 gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac gcctggcact gccgggcgtt
     8341 gttcttttta acttcaggcg ggttacaata gtttccagta agtattctgg aggctgcatc
     8401 catgacacag gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac
     8461 ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt cggcccttga
     8521 tggtaaaacc atccctcact ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg
     8581 cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt gtgatgagcg atgccgaacg
     8641 taccgacgat gatttatacg atacggtgat tggctaccgt ggcggcaact ggatttatga
     8701 gtgggccccg gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac
     8761 tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta
     8821 aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg
     8881 agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta
     8941 gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg
     9001 tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta
     9061 gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat
     9121 acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac agttataatc
     9181 ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt aataactatg
     9241 ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag gaatatttga
     9301 tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga ggttttactt
     9361 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     9421 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     9481 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     9541 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt
     9601 cccaccccat accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt
     9661 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac
     9721 ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg atctgtggat
     9781 ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc caggggtcag
     9841 atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga
     9901 agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga
     9961 tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt
    10021 ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg
    10081 actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc
    10141 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
    10201 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
    10261 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
    10321 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
    10381 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
    10441 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
    10501 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
    10561 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
    10621 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
    10681 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
    10741 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
    10801 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
    10861 cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat gagggacaat
    10921 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
    10981 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
    11041 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
    11101 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
    11161 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
    11221 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
    11281 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
    11341 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
    11401 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
    11461 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
    11521 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
    11581 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
    11641 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
    11701 gagagagaca gagacagatc cattcgatta gtgaa
//
