LOCUS       sequence-351713-        9711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of SOS1 fused to EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9711)
  AUTHORS   Suzuki S, Nakamura A, Hatano Y, Yoshikawa M, Yoshii T, Sawada S, 
            Atsuta-Tsunoda K, Aoki K, Tsukiji S
  TITLE     A chemogenetic platform for controlling plasma membrane signaling 
            and synthetic signal oscillation.
  JOURNAL   Cell Chem Biol. 2022 Sep 15;29(9):1446-1464.e10. doi: 
            10.1016/j.chembiol.2022.06.005. Epub 2022 Jul 13.
  PUBMED    35835118
REFERENCE   2  (bases 1 to 9711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2022.06.005"; journalName: "Cell Chem Biol"; 
            date: "2022-09-15- 15"; volume: "29"; issue: "9"; pages: 
            "1446-1464.e10"
COMMENT     Sequence Label: pCAGGS-EGFP-SOS1
FEATURES             Location/Qualifiers
     source          1..9711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          117..1133
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1057..1079
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1141..1160
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1222..1231
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1228..1230,1231..1233,1234..1944)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1228..1230,1231..1233,1234..1944)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1273..1294)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1534..1553)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1881..1902
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6099..6119)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6099..6117)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6138..6154)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6256..6275)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6321..6376
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6375..6394)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6737..6753)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6761..6777
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6785..6791,6792..6809,6810..6815))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6830..6851
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6910..7105
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6956..7091
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7111..7245
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7148..7167)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7202..7221
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7313..7330)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7484..8072)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7564..7583)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8243..9034,9035..9103))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8866..8885
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9104..9208)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        9239..9618
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt
       61 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
      121 tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc
      181 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
      241 gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc
      301 ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt
      361 gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg
      421 ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg
      481 gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
      541 tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc
      601 ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg
      661 cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc
      721 cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg cccccggagc gccggcggct
      781 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
      841 gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct
      901 ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc
      961 ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg
     1021 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg
     1081 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg
     1141 gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattcata gtagctagca
     1201 gttgctcgag agtacccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
     1261 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     1321 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     1381 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     1441 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     1501 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     1561 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     1621 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     1681 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     1741 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     1801 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     1861 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     1921 ctcggcatgg acgagctgta caagtccgga ctcagatctc gacaaggtag tggtgctggc
     1981 tctggtgctg gtagtggcgc tggttccggt gctggctctg gcgcgcctcg agctcaagct
     2041 tcgcaggcgc agcagctgcc ctacgagttt ttcagcgaag agaacgcgcc caagtggcgg
     2101 ggactactgg tgcctgcgct gaaaaaggtc caggggcaag ttcatcctac tctcgagtct
     2161 aatgatgatg ctcttcagta tgttgaagaa ttaattttgc aattattaaa tatgctatgc
     2221 caagctcagc cccgaagtgc ttcagatgta gaggaacgtg ttcaaaaaag tttccctcat
     2281 ccaattgata aatgggcaat agctgatgcc caatcagcta ttgaaaagag gaagcgaaga
     2341 aaccctttat ctctcccagt agaaaaaatt catcctttat taaaggaggt cctaggttat
     2401 aaaattgacc accaggtttc tgtttacata gtagcagtct tagaatacat ttctgcagac
     2461 attttaaagc tggttgggaa ttatgtaaga aatatacggc attatgaaat tacaaaacaa
     2521 gatattaaag tggcaatgtg tgctgacaag gtattgatgg atatgtttca tcaagatgta
     2581 gaagatatta atatattatc tttaactgac gaagagcctt ccacctcagg agaacaaact
     2641 tactatgatt tggtaaaagc atttatggca gaaattcgac aatatataag ggaactaaat
     2701 ctaattataa aagtttttag agagcccttt gtctccaatt caaaattgtt ttcagctaat
     2761 gatgtagaaa atatatttag tcgcatagta gatatacatg aacttagtgt aaagttactg
     2821 ggccatatag aagatacagt agaaatgaca gatgaaggca gtccccatcc actagtagga
     2881 agctgctttg aagacttagc agaggaactg gcatttgatc catatgaatc gtatgctcga
     2941 gatattttgc gacctggttt tcatgatcgt ttccttagtc agttatcaaa gcctggggca
     3001 gcactttatt tgcagtcaat aggcgaaggt ttcaaagaag ctgttcaata tgttttaccc
     3061 aggctgcttc tggcccctgt ttaccactgt ctccattact ttgaactttt gaagcagtta
     3121 gaagaaaaaa gtgaagatca agaagacaag gaatgtttaa aacaagcaat aacagctttg
     3181 cttaatgttc agagtggtat ggaaaaaata tgttctaaaa gtcttgcaaa acgaagactg
     3241 agtgaatctg catgtcggtt ttatagtcag caaatgaagg ggaaacaact agcaatcaag
     3301 aagatgaacg agattcagaa gaatattgat ggttgggagg gaaaagacat tggacagtgt
     3361 tgtaatgaat ttataatgga aggaactctt acacgtgtag gagccaaaca tgagagacac
     3421 atatttctct ttgatggctt aatgatttgc tgtaaatcaa atcatgggca gccaagactt
     3481 cctggtgcta gcaatgcaga atatcgtctt aaagaaaagt tttttatgcg aaaggtacaa
     3541 attaatgata aagatgacac caatgaatac aagcatgctt ttgaaataat tttaaaagat
     3601 gaaaatagtg ttatattttc tgccaagtca gctgaagaga aaaacaattg gatggcagca
     3661 ttgatatctt tacagtaccg gagtacactg gaaaggatgc ttgatgtaac aatgctacag
     3721 gaagagaaag aggagcagat gaggctgcct agtgctgatg tttatagatt tgcagagcct
     3781 gactctgaag agaatattat atttgaagag aacatgcagc ccaaggctgg aattccaatt
     3841 atcaaagcag gaactgttat taaacttata gagaggctta cgtaccatat gtacgcagat
     3901 cccaattttg ttcggacatt tcttacaaca tacagatcct tttgcaaacc tcaagaacta
     3961 ctgagtctta taatagaaag gtttgaaatt ccagagcctg agccaacaga agctgatcgc
     4021 atagctatag agaatggaga tcaacccttg agtgcagaac tgaaaagatt tagaaaagaa
     4081 tatatacagc ctgtgcaact gcgagtatta aatgtatgtc ggcactgggt agagcaccac
     4141 ttctatgatt ttgaaagaga tgcatatctt ttgcaacgaa tggaagaatt tattggaaca
     4201 gtaagaggta aagcaatgaa aaaatgggtt gaatccatca ctaaaataat ccaaaggaaa
     4261 aaaattgcaa gagacaatgg accaggtcat aatattacat ttcagagttc acctcccaca
     4321 gttgagtggc atataagcag acctgggcac atagagactt ttgacctgct caccttacac
     4381 ccaatagaaa ttgctcgaca actcacttta cttgaatcag atctataccg agctgtacag
     4441 ccatcagaat tagttggaag tgtgtggaca aaagaagaca aagaaattaa ctctcctaat
     4501 cttctgaaaa tgattcgaca taccaccaac ctcactctgt ggtttgagaa atgtattgta
     4561 gaaactgaaa atttagaaga aagagtagct gtggtgagtc gaattattga gattctacaa
     4621 gtctttcaag agttgaacaa ctttaatggt gtccttgagg ttgtcagtgc tatgaattca
     4681 tcacctgttt acagactaga ccacacattt gagcaaatac caagtcgcca gaagaaaatt
     4741 ttagaagaag ctcatgaatt gagtgaagat cactataaga aatatttggc aaaactcagg
     4801 tctattaatc caccatgtgt gcctttcttt ggaatttatc tcactaatat cttgaaaaca
     4861 gaagaaggca accctgaggt cctaaaaaga catggaaaag agcttataaa ctttagcaaa
     4921 aggaggaaag tagcagaaat aacaggagag atccagcagt accaaaatca gccttactgt
     4981 ttacgagtag aatcagatat caaaaggttc tttgaaaact tgaatccgat gggaaatagc
     5041 atggagaagg aattcacaga ttatcttttc aacaaatccc tagaaataga accacgaaac
     5101 cctaagcctc tcccaagatt tccaaaaaaa tatagctatc ccctaaaatc tcctggtgtt
     5161 cgtccatcaa acccaagacc aggtaccatg aggcatccca cacctctgca gcaggagcca
     5221 aggaaaatta gttatagtag gatccctgaa agtgaaacag aaagtacagc atctgcacca
     5281 aattctccaa gaacaccgtt aacacctccg cctgcttctg gtgcttccag taccacagat
     5341 gtttgcagtg tatttgattc cgatcattcg agcccttttc actcaagcaa tgataccgtc
     5401 tttatccaag ttactctgcc ccatggccca agatctgctt ctgtatcatc tataagttta
     5461 accaaaggca ctgatgaagt gcctgtccct cctcctgttc ctccacgaag acgaccagaa
     5521 tctgccccag cagaatcttc accatctaag attatgtcta agcatttgga cagtccccca
     5581 gccattcctc ctaggcaacc cacatcaaaa gcctattcac cacgatattc aatatcagac
     5641 cggacctcta tctcagaccc tcctgaaagc cctcccttat taccaccacg agaacctgtg
     5701 aggacacctg atgttttctc aagctcacca ctacatctcc aacctccccc tttgggcaaa
     5761 aaaagtgacc atggcaatgc cttcttccca aacagccctt ccccctttac accacctcct
     5821 cctcaaacac cttctcctca cggcacaaga aggcatctgc catcaccacc attgacacaa
     5881 gaagtggacc ttcattccat tgctgggccg cctgttcctc cacgacaaag cacttctcaa
     5941 catatcccta aactccctcc aaaaacttac aaaagggagc acacacaccc atccatgcac
     6001 agagatggac caccactgtt ggagaatgcc cattcttcct aaaccgcggg cccgggatac
     6061 tcgcggccgc caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg
     6121 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctaattcac
     6181 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
     6241 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
     6301 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     6361 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
     6421 tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg
     6481 ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca
     6541 tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc
     6601 tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg
     6661 actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc tgcagcccaa
     6721 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     6781 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     6841 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     6901 agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     6961 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     7021 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     7081 ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta taatggttac
     7141 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     7201 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg cattaatgaa
     7261 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     7321 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     7381 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     7441 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     7501 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     7561 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7621 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7681 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7741 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7801 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7861 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7921 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7981 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     8041 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     8101 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     8161 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     8221 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     8281 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     8341 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     8401 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     8461 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     8521 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     8581 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8641 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8701 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     8761 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8821 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     8881 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     8941 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     9001 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     9061 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     9121 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     9181 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga
     9241 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     9301 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     9361 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     9421 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     9481 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     9541 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     9601 gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc
     9661 cccccctccc cacccccaat tttgtattta tttatttttt aattattttg t
//
