LOCUS       sequence-351716-       11844 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of miRFP670-eDHFR(69K6).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11844)
  AUTHORS   Suzuki S, Nakamura A, Hatano Y, Yoshikawa M, Yoshii T, Sawada S, 
            Atsuta-Tsunoda K, Aoki K, Tsukiji S
  TITLE     A chemogenetic platform for controlling plasma membrane signaling 
            and synthetic signal oscillation.
  JOURNAL   Cell Chem Biol. 2022 Sep 15;29(9):1446-1464.e10. doi: 
            10.1016/j.chembiol.2022.06.005. Epub 2022 Jul 13.
  PUBMED    35835118
REFERENCE   2  (bases 1 to 11844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2022.06.005"; journalName: "Cell Chem Biol"; 
            date: "2022-09-15- 15"; volume: "29"; issue: "9"; pages: 
            "1446-1464.e10"
COMMENT     Sequence Label: pCSIIpuro-miRFP670-eDHFR(69K6)
FEATURES             Location/Qualifiers
     source          1..11844
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(32..51)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        223..602
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        604..802
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     757..777
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             820..1000
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1047..1172
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1664..1897
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2082..2126
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2400..2517
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2573..3735
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2804..3726
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3683..3703
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3757..3766
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3763..4707
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 670 nm (Shcherbakova et al., 2016)"
                     /label=miRFP670
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     misc_feature    5341..5890
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5485..5502)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5712..5731
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5893..6492
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5893..5912)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6389..6409
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6527..7115
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6580..6600)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6998..7009)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7634..7814
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7840..7857)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7846..8070
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8116..8544
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8203..8222)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8413..8434
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8553..8573)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8558..8887
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8738..8873
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8800..8819
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8935..8982
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9001..9375
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9505..9626
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9542..9561)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9596..9615
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9675..9691)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9675..9691)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9688..9710)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9699..9715
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9723..9729,9730..9747,9748..9753))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9768..9789
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9906..9923)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10077..10665)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10157..10176)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10836..11627,11628..11696))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11459..11478
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11697..11801)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
       61 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
      121 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
      181 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta
      241 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      301 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      361 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      421 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      481 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      541 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      601 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
      661 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      721 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
      781 ggtgggaggt ctatataagc agcgcgtttt gcctgtactg ggtctctctg gttagaccag
      841 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
      901 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
      961 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     1021 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     1081 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     1141 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     1201 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     1261 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     1321 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     1381 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     1441 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     1501 cacagcaagg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     1561 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     1621 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     1681 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     1741 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     1801 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     1861 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     1921 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     1981 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     2041 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     2101 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     2161 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     2221 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     2281 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     2341 acagagacag atccattcga ttagtgaacg gatctacaaa tggcagtatt catccacaat
     2401 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     2461 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
     2521 gtttattaca gggacagcag aaattcactt tgaattaatt caagcttcgt gaggctccgg
     2581 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
     2641 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
     2701 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
     2761 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
     2821 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
     2881 ctggctccag tacgtgattc ttgatcccga gctggagcca ggggcgggcc ttgcgcttta
     2941 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg
     3001 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa
     3061 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc
     3121 caggatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg
     3181 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg
     3241 gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc
     3301 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg
     3361 cttcccggcc ctgctccagg gggctcaaaa tggaggacgc ggcgctcggg agagcgggcg
     3421 ggtgagtcac ccacacaaag gaaaggggcc tttccgtcct cagccgtcgc ttcatgtgac
     3481 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctggagctt ttggagtacg
     3541 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg
     3601 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt
     3661 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca
     3721 tttcaggtgt cgtgaacacg ctaccggtcc gaattggcca ccatggtagc aggtcatgcc
     3781 tctggcagcc ccgcattcgg gaccgcctct cattcgaatt gcgaacatga agagatccac
     3841 ctcgccggct cgatccagcc gcatggcgcg cttctggtcg tcagcgaaca tgatcatcgc
     3901 gtcatccagg ccagcgccaa cgccgcggaa tttctgaatc tcggaagcgt actcggcgtt
     3961 ccgctcgccg agatcgacgg cgatctgttg atcaagatcc tgccgcatct cgatcccacc
     4021 gccgaaggca tgccggtcgc ggtgcgctgc cggatcggca atccctctac ggagtactgc
     4081 ggtctgatgc atcggcctcc ggaaggcggg ctgatcatcg aactcgaacg tgccggcccg
     4141 tcgatcgatc tgtcaggcac gctggcgccg gcgctggagc ggatccgcac ggcgggttca
     4201 ctgcgcgcgc tgtgcgatga caccgtgctg ctgtttcagc agtgcaccgg ctacgaccgg
     4261 gtgatggtgt atcgtttcga tgagcaaggc cacggcctgg tattctccga gtgccatgtg
     4321 cctgggctcg aatcctattt cggcaaccgc tatccgtcgt cgactgtccc gcagatggcg
     4381 cggcagctgt acgtgcggca gcgcgtccgc gtgctggtcg acgtcaccta tcagccggtg
     4441 ccgctggagc cgcggctgtc gccgctgacc gggcgcgatc tcgacatgtc gggctgcttc
     4501 ctgcgctcga tgtcgccgtg ccatctgcag ttcctgaagg acatgggcgt gcgcgccacc
     4561 ctggcggtgt cgctggtggt cggcggcaag ctgtggggcc tggttgtctg tcaccattat
     4621 ctgccgcgct tcatccgttt cgagctgcgg gcgatctgca aacggctcgc cgaaaggatc
     4681 gcgacgcgga tcaccgcgct tgagagcctc tacaagtctg ggtccgcagc cagtagcgcc
     4741 ggaagtatga tcagtctgat tgcggcgtta gcggtagatc gcgttatcgg catggaaaac
     4801 gccatgccgt ggaacctgcc tgccgatctc gcctggttta aacgcaacac cttaaataaa
     4861 cccgtgatta tgggccgcca tacctgggaa tcaatcggtc gtccgttgcc aggacgcaaa
     4921 aatattatcc tcagcagtca accgggtacg gacaaaaaaa agaaaaagaa agatcgcgta
     4981 acgtgggtga agtcggtgga tgaagccatc gcggcgtgtg gtgacgtacc agaaatcatg
     5041 gtgattggcg gcggtcgcgt ttatgaacag ttcttgccaa aagcgcaaaa actgtatctg
     5101 acgcatatcg acgcagaagt ggaaggcgac acccatttcc cggattacga gccggatgac
     5161 tgggaatcgg tattcagcga attccacgat gctgatgcgc agaactctca cagctattgc
     5221 tttgagattc tggagcggcg gctgtacaag taaagcggcc gcggctctag aggatccgtt
     5281 aactaactta agctagcaac ggtttccctc tagcgggatc aattccgccc ccccccccta
     5341 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
     5401 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
     5461 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     5521 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     5581 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     5641 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
     5701 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     5761 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
     5821 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     5881 cacgataata ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     5941 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     6001 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     6061 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     6121 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     6181 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     6241 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     6301 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     6361 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     6421 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     6481 cccggtgcct gactagagtt aacatcgagg gatcaagctt atcgataatc aacctctgga
     6541 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     6601 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     6661 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     6721 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     6781 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     6841 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     6901 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     6961 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     7021 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     7081 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgag acctagaaaa
     7141 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga
     7201 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat
     7261 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     7321 gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg
     7381 ctacttccct gattggcaga actacacacc agggccaggg atcagatatc cactgacctt
     7441 tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg
     7501 agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
     7561 agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca
     7621 tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     7681 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     7741 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     7801 gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     7861 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     7921 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     7981 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     8041 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     8101 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     8161 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     8221 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     8281 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     8341 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     8401 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     8461 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     8521 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     8581 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     8641 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     8701 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     8761 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     8821 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     8881 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac
     8941 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     9001 atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc
     9061 gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt
     9121 gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac
     9181 aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag
     9241 gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag
     9301 ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc
     9361 gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
     9421 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     9481 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     9541 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     9601 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     9661 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     9721 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     9781 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     9841 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     9901 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     9961 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
    10021 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    10081 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    10141 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    10201 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    10261 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    10321 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    10381 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    10441 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    10501 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    10561 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    10621 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    10681 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    10741 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    10801 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    10861 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    10921 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    10981 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    11041 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    11101 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    11161 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    11221 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    11281 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    11341 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    11401 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    11461 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    11521 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    11581 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    11641 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    11701 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    11761 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    11821 ccacctgacg tcgacggatc ggga
//
