LOCUS       sequence-35172-e         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Thaler C, Koushik SV, Blank PS, Vogel SS
  TITLE     Quantitative multiphoton spectral imaging and its use for measuring 
            resonance energy transfer.
  JOURNAL   Biophys J. 2005 Oct . 89(4):2736-49.
  PUBMED    16040744
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biophys J. 
            2005 Oct . 89(4):2736-49."
COMMENT     Sequence Label: CVC
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(148..858,859..861))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(190..211)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     539..558
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     798..819
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ataagctgca taacagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
       61 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
      121 gatcagttat ctagatccgg tggatccctt gtacagctcg tccatgccga gagtgatccc
      181 ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct
      241 cagcttggac tgggtgctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat
      301 gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg
      361 gatcttgaag ttggccttga tgccgttctt ctgcttgtcg gcggtgatat agacgttgtc
      421 gctgatggcg ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat
      481 gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt
      541 cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat
      601 ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcgggcga agcactgcac
      661 gccccaggtc agggtggtca cgagggtggg ccagggcacg ggcagcttgc cggtggtgca
      721 gatgaacttc agggtcagct tgccgtaagt ggcatcgccc tcgccctcgc cggacacgct
      781 gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca ccccggtgaa
      841 cagctcctcg cccttgctca cccgtcgact gcagaattcc ttgtacagct cgtccatgcc
      901 caaga
//
