LOCUS       sequence-351729-        8484 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of WLS-HA protein (WT).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8484)
  AUTHORS   Chai G, Szenker-Ravi E, Chung C, Li Z, Wang L, Khatoo M, Marshall T,
            Jiang N, Yang X, McEvoy-Venneri J, Stanley V, Anzenberg P, Lang N, 
            Wazny V, Yu J, Virshup DM, Nygaard R, Mancia F, Merdzanic R, 
            Toralles MBP, Pitanga PML, Puri RD, Hernan R, Chung WK, 
            Bertoli-Avella AM, Al-Sannaa N, Zaki MS, Willert K, Reversade B, 
            Gleeson JG
  TITLE     A Human Pleiotropic Multiorgan Condition Caused by Deficient Wnt 
            Secretion.
  JOURNAL   N Engl J Med. 2021 Sep 30;385(14):1292-1301. doi: 
            10.1056/NEJMoa2033911.
  PUBMED    34587386
REFERENCE   2  (bases 1 to 8484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "N Engl J Med"; date: "2021-09-30- 30"; volume: "385"; issue: "14"; 
            pages: "1292-1301"
COMMENT     Sequence Label: pcDNA3-WT-WLS-HA-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..8484
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      901..910
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2548..2574
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(2648..3222,3223..3225,3226..3234)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2815..2832)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3042..3061
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3235..3237,3238..3240,3241..3954)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3280..3301)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3541..3560)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3888..3909
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4075..4092)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4081..4305
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4351..4779
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4438..4457)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4648..4669
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4788..4808)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4793..5122
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4973..5108
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5035..5054
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5189..5983
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5243..5262)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5853..5872
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6157..6278
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6194..6213)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6248..6267
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6327..6343)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6327..6343)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6340..6362)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6351..6367
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6375..6381,6382..6399,6400..6405))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6420..6441
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6558..6575)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6729..7317)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6809..6828)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7488..8279,8280..8348))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8111..8130
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8349..8453)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gccaccatgg ctggggcaat tatagaaaac atgagcacca agaagctgtg cattgttggt
      961 gggattctgc tcgtgttcca aatcatcgcc tttctggtgg gaggcttgat tgctccaggg
     1021 cccacaacgg cagtgtccta catgtcggtg aaatgtgtgg atgcccgtaa gaaccatcac
     1081 aagacaaaat ggttcgtgcc ttggggaccc aatcattgtg acaagatccg agacattgaa
     1141 gaggcaattc caagggaaat tgaagccaat gacatcgtgt tttctgttca cattcccctc
     1201 ccccacatgg agatgagtcc ttggttccaa ttcatgctgt ttatcctgca gctggacatt
     1261 gccttcaagc taaacaacca aatcagagaa aatgcagaag tctccatgga cgtttccctg
     1321 gcttaccgtg atgacgcatt tgctgagtgg actgaaatgg cccatgaaag agtaccacgg
     1381 aaactcaaat gcaccttcac atctcccaag actccagagc atgagggccg ttactatgaa
     1441 tgtgatgtcc ttcctttcat ggaaattggg tctgtggccc ataagtttta ccttttaaac
     1501 atccggctgc ctgtgaatga gaagaagaaa atcaatgtgg gaattgggga gataaaggat
     1561 atccggttgg tggggatcca ccaaaatgga ggcttcacca aggtgtggtt tgccatgaag
     1621 accttcctta cgcccagcat cttcatcatt atggtgtggt attggaggag gatcaccatg
     1681 atgtcccgac ccccagtgct tctggaaaaa gtcatctttg cccttgggat ttccatgacc
     1741 tttatcaata tcccagtgga atggttttcc atcgggtttg actggacctg gatgctgctg
     1801 tttggtgaca tccgacaggg catcttctat gcgatgcttc tgtccttctg gatcatcttc
     1861 tgtggcgagc acatgatgga tcagcacgag cggaaccaca tcgcagggta ttggaagcaa
     1921 gtcggaccca ttgccgttgg ctccttctgc ctcttcatat ttgacatgtg tgagagaggg
     1981 gtacaactca cgaatccctt ctacagtatc tggactacag acattggaac agagctggcc
     2041 atggccttca tcatcgtggc tggaatctgc ctctgcctct acttcctgtt tctatgcttc
     2101 atggtatttc aggtgtttcg gaacatcagt gggaagcagt ccagcctgcc agctatgagc
     2161 aaagtccggc ggctacacta tgaggggcta atttttaggt tcaagttcct catgcttatc
     2221 accttggcct gcgctgccat gactgtcatc ttcttcatcg ttagtcaggt aacggaaggc
     2281 cattggaaat ggggcggcgt cacagtccaa gtgaacagtg cctttttcac aggcatctat
     2341 gggatgtgga atctgtatgt ctttgctctg atgttcttgt atgcaccatc ccataaaaac
     2401 tatggagaag accagtccaa tggcgatctg ggtgtccata gtggggaaga actccagctc
     2461 accaccacta tcacccatgt ggacggaccc actgagatct acaagttgac ccgcaaggag
     2521 gcccaggagg gtggcggagg tggcggttat ccttacgacg tgcctgacta cgcctaggaa
     2581 ttctgcagat atccagcaca gtggcggccg ctcgaggtta acgaattccg cccccccccc
     2641 cccccccccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
     2701 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     2761 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     2821 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     2881 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     2941 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     3001 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     3061 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     3121 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     3181 accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac aaccatggtg
     3241 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     3301 gtaaacggcc acaagttcag cgtgtctggc gagggcgagg gcgatgccac ctacggcaag
     3361 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     3421 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     3481 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     3541 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     3601 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     3661 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     3721 aaggcgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     3781 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     3841 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     3901 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaatgaatt
     3961 aattaagaat tatcaccgct tctattcagc cagtaaggcc tgtctttaat ggcctccggc
     4021 atgagacact ttctagagtc gagtctagag ggcccgttta aacccgctga tcagcctcga
     4081 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     4141 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     4201 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     4261 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     4321 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     4381 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     4441 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     4501 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     4561 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     4621 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     4681 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     4741 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     4801 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     4861 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     4921 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     4981 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     5041 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     5101 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     5161 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     5221 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     5281 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     5341 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     5401 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     5461 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     5521 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     5581 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     5641 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     5701 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     5761 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     5821 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     5881 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     5941 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     6001 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     6061 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     6121 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     6181 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     6241 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     6301 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     6361 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     6421 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     6481 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     6541 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     6601 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     6661 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     6721 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     6781 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     6841 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     6901 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     6961 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7021 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7081 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7141 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     7201 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7261 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7321 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     7381 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     7441 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     7501 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     7561 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     7621 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     7681 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     7741 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     7801 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     7861 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     7921 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     7981 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     8041 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     8101 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     8161 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     8221 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     8281 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     8341 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     8401 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     8461 ccccgaaaag tgccacctga cgtc
//
