LOCUS       sequence-351731-        8468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of WLS-HA protein (R536C mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8468)
  AUTHORS   Chai G, Szenker-Ravi E, Chung C, Li Z, Wang L, Khatoo M, Marshall T,
            Jiang N, Yang X, McEvoy-Venneri J, Stanley V, Anzenberg P, Lang N, 
            Wazny V, Yu J, Virshup DM, Nygaard R, Mancia F, Merdzanic R, 
            Toralles MBP, Pitanga PML, Puri RD, Hernan R, Chung WK, 
            Bertoli-Avella AM, Al-Sannaa N, Zaki MS, Willert K, Reversade B, 
            Gleeson JG
  TITLE     A Human Pleiotropic Multiorgan Condition Caused by Deficient Wnt 
            Secretion.
  JOURNAL   N Engl J Med. 2021 Sep 30;385(14):1292-1301. doi: 
            10.1056/NEJMoa2033911.
  PUBMED    34587386
REFERENCE   2  (bases 1 to 8468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "N Engl J Med"; date: "2021-09-30- 30"; volume: "385"; issue: "14"; 
            pages: "1292-1301"
COMMENT     Sequence Label: pcDNA3-R536C-WLS-HA-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..8468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(6..580,581..583,584..592)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(173..190)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     400..419
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(593..595,596..598,599..1312)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(638..659)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(899..918)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1246..1267
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1433..1450)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1439..1663
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1709..2137
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1796..1815)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2006..2027
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2146..2166)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2151..2480
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2331..2466
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2393..2412
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2547..3341
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2601..2620)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3211..3230
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3515..3636
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3552..3571)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3606..3625
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3685..3701)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3685..3701)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3698..3720)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3709..3725
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3733..3739,3740..3757,3758..3763))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3778..3799
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3916..3933)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4087..4675)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4167..4186)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4846..5637,5638..5706))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5469..5488
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5707..5811)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5886..5905)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6077..6456
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6457..6660
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6611..6631
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6705..6724
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6705..6723
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      6743..6752
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8390..8416
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ccccccccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
       61 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
      121 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
      181 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
      241 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
      301 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
      361 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
      421 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
      481 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
      541 cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa ccatggtgag
      601 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
      661 aaacggccac aagttcagcg tgtctggcga gggcgagggc gatgccacct acggcaagct
      721 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
      781 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
      841 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
      901 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
      961 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     1021 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     1081 ggcgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     1141 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     1201 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     1261 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aatgaattaa
     1321 ttaagaatta tcaccgcttc tattcagcca gtaaggcctg tctttaatgg cctccggcat
     1381 gagacacttt ctagagtcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact
     1441 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     1501 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     1561 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     1621 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     1681 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     1741 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     1801 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     1861 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     1921 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     1981 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     2041 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     2101 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     2161 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     2221 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     2281 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     2341 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     2401 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     2461 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     2521 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     2581 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     2641 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     2701 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     2761 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     2821 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     2881 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     2941 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     3001 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     3061 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     3121 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     3181 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     3241 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     3301 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     3361 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     3421 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     3481 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     3541 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     3601 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     3661 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     3721 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     3781 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     3841 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     3901 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     3961 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     4021 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     4081 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     4141 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     4201 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     4261 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     4321 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     4381 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     4441 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     4501 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     4561 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     4621 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     4681 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     4741 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     4801 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     4861 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     4921 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     4981 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     5041 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     5101 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     5161 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     5221 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     5281 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     5341 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     5401 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     5461 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     5521 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     5581 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     5641 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     5701 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     5761 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     5821 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
     5881 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
     5941 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
     6001 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
     6061 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
     6121 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     6181 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     6241 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     6301 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     6361 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     6421 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     6481 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     6541 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     6601 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
     6661 ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat
     6721 agggagaccc aagctggcta gcgccaccat ggctggggca attatagaaa acatgagcac
     6781 caagaagctg tgcattgttg gtgggattct gctcgtgttc caaatcatcg cctttctggt
     6841 gggaggcttg attgctccag ggcccacaac ggcagtgtcc tacatgtcgg tgaaatgtgt
     6901 ggatgcccgt aagaaccatc acaagacaaa atggttcgtg ccttggggac ccaatcattg
     6961 tgacaagatc cgagacattg aagaggcaat tccaagggaa attgaagcca atgacatcgt
     7021 gttttctgtt cacattcccc tcccccacat ggagatgagt ccttggttcc aattcatgct
     7081 gtttatcctg cagctggaca ttgccttcaa gctaaacaac caaatcagag aaaatgcaga
     7141 agtctccatg gacgtttccc tggcttaccg tgatgacgca tttgctgagt ggactgaaat
     7201 ggcccatgaa agagtaccac ggaaactcaa atgcaccttc acatctccca agactccaga
     7261 gcatgagggc cgttactatg aatgtgatgt ccttcctttc atggaaattg ggtctgtggc
     7321 ccataagttt taccttttaa acatccggct gcctgtgaat gagaagaaga aaatcaatgt
     7381 gggaattggg gagataaagg atatccggtt ggtggggatc caccaaaatg gaggcttcac
     7441 caaggtgtgg tttgccatga agaccttcct tacgcccagc atcttcatca ttatggtgtg
     7501 gtattggagg aggatcacca tgatgtcccg acccccagtg cttctggaaa aagtcatctt
     7561 tgcccttggg atttccatga cctttatcaa tatcccagtg gaatggtttt ccatcgggtt
     7621 tgactggacc tggatgctgc tgtttggtga catccgacag ggcatcttct atgcgatgct
     7681 tctgtccttc tggatcatct tctgtggcga gcacatgatg gatcagcacg agcggaacca
     7741 catcgcaggg tattggaagc aagtcggacc cattgccgtt ggctccttct gcctcttcat
     7801 atttgacatg tgtgagagag gggtacaact cacgaatccc ttctacagta tctggactac
     7861 agacattgga acagagctgg ccatggcctt catcatcgtg gctggaatct gcctctgcct
     7921 ctacttcctg tttctatgct tcatggtatt tcaggtgttt cggaacatca gtgggaagca
     7981 gtccagcctg ccagctatga gcaaagtccg gcggctacac tatgaggggc taatttttag
     8041 gttcaagttc ctcatgctta tcaccttggc ctgcgctgcc atgactgtca tcttcttcat
     8101 cgttagtcag gtaacggaag gccattggaa atggggcggc gtcacagtcc aagtgaacag
     8161 tgcctttttc acaggcatct atgggatgtg gaatctgtat gtctttgctc tgatgttctt
     8221 gtatgcacca tcccataaaa actatggaga agaccagtcc aatggcgatc tgggtgtcca
     8281 tagtggggaa gaactccagc tcaccaccac tatcacccat gtggacggac ccactgagat
     8341 ctacaagttg acctgcaagg aggcccagga gggtggcgga ggtggcggtt atccttacga
     8401 cgtgcctgac tacgcctagg aattctgcag atatccagca cagtggcggc cgctcgaggt
     8461 taacgaat
//
