LOCUS       sequence-351732-        8458 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of WLS-HA protein (I531T mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8458)
  AUTHORS   Chai G, Szenker-Ravi E, Chung C, Li Z, Wang L, Khatoo M, Marshall T,
            Jiang N, Yang X, McEvoy-Venneri J, Stanley V, Anzenberg P, Lang N, 
            Wazny V, Yu J, Virshup DM, Nygaard R, Mancia F, Merdzanic R, 
            Toralles MBP, Pitanga PML, Puri RD, Hernan R, Chung WK, 
            Bertoli-Avella AM, Al-Sannaa N, Zaki MS, Willert K, Reversade B, 
            Gleeson JG
  TITLE     A Human Pleiotropic Multiorgan Condition Caused by Deficient Wnt 
            Secretion.
  JOURNAL   N Engl J Med. 2021 Sep 30;385(14):1292-1301. doi: 
            10.1056/NEJMoa2033911.
  PUBMED    34587386
REFERENCE   2  (bases 1 to 8458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "N Engl J Med"; date: "2021-09-30- 30"; volume: "385"; issue: "14"; 
            pages: "1292-1301"
COMMENT     Sequence Label: pcDNA3-I531T-WLS-HA-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..8458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        110..489
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        490..693
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     644..664
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     738..757
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        738..756
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      776..785
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2423..2449
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    2502..3070
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2520..3072
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2664..2681)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2891..2910
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3084..3086,3087..3089,3090..3803)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3129..3150)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3390..3409)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3737..3758
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3924..3941)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3930..4154
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4200..4628
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4287..4306)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4497..4518
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4637..4657)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4642..4971
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4822..4957
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4884..4903
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5038..5832
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5092..5111)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5702..5721
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6006..6127
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6043..6062)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6097..6116
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6176..6192)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6176..6192)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6189..6211)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6200..6216
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6224..6230,6231..6248,6249..6254))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6269..6290
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6407..6424)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6578..7166)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6658..6677)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7337..8128,8129..8197))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7960..7979
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8198..8302)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8377..8396)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
       61 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
      121 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      181 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      241 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      301 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      361 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      421 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      481 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      541 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      601 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      661 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      721 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgccac
      781 catggctggg gcaattatag aaaacatgag caccaagaag ctgtgcattg ttggtgggat
      841 tctgctcgtg ttccaaatca tcgcctttct ggtgggaggc ttgattgctc cagggcccac
      901 aacggcagtg tcctacatgt cggtgaaatg tgtggatgcc cgtaagaacc atcacaagac
      961 aaaatggttc gtgccttggg gacccaatca ttgtgacaag atccgagaca ttgaagaggc
     1021 aattccaagg gaaattgaag ccaatgacat cgtgttttct gttcacattc ccctccccca
     1081 catggagatg agtccttggt tccaattcat gctgtttatc ctgcagctgg acattgcctt
     1141 caagctaaac aaccaaatca gagaaaatgc agaagtctcc atggacgttt ccctggctta
     1201 ccgtgatgac gcatttgctg agtggactga aatggcccat gaaagagtac cacggaaact
     1261 caaatgcacc ttcacatctc ccaagactcc agagcatgag ggccgttact atgaatgtga
     1321 tgtccttcct ttcatggaaa ttgggtctgt ggcccataag ttttaccttt taaacatccg
     1381 gctgcctgtg aatgagaaga agaaaatcaa tgtgggaatt ggggagataa aggatatccg
     1441 gttggtgggg atccaccaaa atggaggctt caccaaggtg tggtttgcca tgaagacctt
     1501 ccttacgccc agcatcttca tcattatggt gtggtattgg aggaggatca ccatgatgtc
     1561 ccgaccccca gtgcttctgg aaaaagtcat ctttgccctt gggatttcca tgacctttat
     1621 caatatccca gtggaatggt tttccatcgg gtttgactgg acctggatgc tgctgtttgg
     1681 tgacatccga cagggcatct tctatgcgat gcttctgtcc ttctggatca tcttctgtgg
     1741 cgagcacatg atggatcagc acgagcggaa ccacatcgca gggtattgga agcaagtcgg
     1801 acccattgcc gttggctcct tctgcctctt catatttgac atgtgtgaga gaggggtaca
     1861 actcacgaat cccttctaca gtatctggac tacagacatt ggaacagagc tggccatggc
     1921 cttcatcatc gtggctggaa tctgcctctg cctctacttc ctgtttctat gcttcatggt
     1981 atttcaggtg tttcggaaca tcagtgggaa gcagtccagc ctgccagcta tgagcaaagt
     2041 ccggcggcta cactatgagg ggctaatttt taggttcaag ttcctcatgc ttatcacctt
     2101 ggcctgcgct gccatgactg tcatcttctt catcgttagt caggtaacgg aaggccattg
     2161 gaaatggggc ggcgtcacag tccaagtgaa cagtgccttt ttcacaggca tctatgggat
     2221 gtggaatctg tatgtctttg ctctgatgtt cttgtatgca ccatcccata aaaactatgg
     2281 agaagaccag tccaatggcg atctgggtgt ccatagtggg gaagaactcc agctcaccac
     2341 cactatcacc catgtggacg gacccactga gacctacaag ttgacccgca aggaggccca
     2401 ggagggtggc ggaggtggcg gttatcctta cgacgtgcct gactacgcct aggaattctg
     2461 cagatatcca gcacagtggc ggccgctcga ggttaacgaa ttcccctccc ccccccctaa
     2521 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     2581 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     2641 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     2701 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     2761 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     2821 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     2881 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     2941 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     3001 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     3061 acgatgataa tatggccaca accatggtga gcaagggcga ggagctgttc accggggtgg
     3121 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtctggcg
     3181 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     3241 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     3301 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     3361 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     3421 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     3481 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     3541 tcatggccga caagcagaag aacggcatca aggcgaactt caagatccgc cacaacatcg
     3601 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     3661 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     3721 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     3781 gcatggacga gctgtacaag taatgaatta attaagaatt atcaccgctt ctattcagcc
     3841 agtaaggcct gtctttaatg gcctccggca tgagacactt tctagagtcg agtctagagg
     3901 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     3961 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     4021 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     4081 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     4141 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     4201 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     4261 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     4321 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     4381 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     4441 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     4501 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     4561 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     4621 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     4681 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     4741 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     4801 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     4861 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     4921 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     4981 ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg
     5041 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     5101 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     5161 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     5221 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     5281 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     5341 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     5401 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     5461 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     5521 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     5581 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     5641 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     5701 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     5761 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     5821 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
     5881 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     5941 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     6001 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     6061 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     6121 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     6181 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     6241 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     6301 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     6361 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     6421 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     6481 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6541 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6601 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6661 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6721 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6781 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6841 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6901 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6961 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     7021 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7081 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     7141 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7201 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     7261 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     7321 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     7381 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     7441 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     7501 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     7561 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     7621 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     7681 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     7741 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     7801 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7861 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7921 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     7981 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     8041 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     8101 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     8161 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     8221 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     8281 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     8341 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
     8401 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagc
//
