LOCUS       sequence-351734-        8454 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of WLS-HA protein (Y392C mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8454)
  AUTHORS   Chai G, Szenker-Ravi E, Chung C, Li Z, Wang L, Khatoo M, Marshall T,
            Jiang N, Yang X, McEvoy-Venneri J, Stanley V, Anzenberg P, Lang N, 
            Wazny V, Yu J, Virshup DM, Nygaard R, Mancia F, Merdzanic R, 
            Toralles MBP, Pitanga PML, Puri RD, Hernan R, Chung WK, 
            Bertoli-Avella AM, Al-Sannaa N, Zaki MS, Willert K, Reversade B, 
            Gleeson JG
  TITLE     A Human Pleiotropic Multiorgan Condition Caused by Deficient Wnt 
            Secretion.
  JOURNAL   N Engl J Med. 2021 Sep 30;385(14):1292-1301. doi: 
            10.1056/NEJMoa2033911.
  PUBMED    34587386
REFERENCE   2  (bases 1 to 8454)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "N Engl J Med"; date: "2021-09-30- 30"; volume: "385"; issue: "14"; 
            pages: "1292-1301"
COMMENT     Sequence Label: pcDNA3-Y392C-WLS-HA-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..8454
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        180..559
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        560..763
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     714..734
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     808..827
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        808..826
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      846..855
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2493..2519
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    2586..3138
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2730..2747)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2957..2976
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3150..3152,3153..3155,3156..3869)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3195..3216)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3456..3475)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3803..3824
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3990..4007)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3996..4220
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4266..4694
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4353..4372)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4563..4584
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4703..4723)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4708..5037
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4888..5023
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4950..4969
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5104..5898
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5158..5177)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5768..5787
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6072..6193
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6109..6128)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6163..6182
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6242..6258)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6242..6258)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6255..6277)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6266..6282
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6290..6296,6297..6314,6315..6320))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6335..6356
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6473..6490)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6644..7232)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6724..6743)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7403..8194,8195..8263))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8026..8045
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8264..8368)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(8443..8454,1..8))
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
       61 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
      121 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
      181 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
      241 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      301 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      361 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      421 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      481 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      541 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      601 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      661 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      721 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga
      781 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg
      841 ctagcgccac catggctggg gcaattatag aaaacatgag caccaagaag ctgtgcattg
      901 ttggtgggat tctgctcgtg ttccaaatca tcgcctttct ggtgggaggc ttgattgctc
      961 cagggcccac aacggcagtg tcctacatgt cggtgaaatg tgtggatgcc cgtaagaacc
     1021 atcacaagac aaaatggttc gtgccttggg gacccaatca ttgtgacaag atccgagaca
     1081 ttgaagaggc aattccaagg gaaattgaag ccaatgacat cgtgttttct gttcacattc
     1141 ccctccccca catggagatg agtccttggt tccaattcat gctgtttatc ctgcagctgg
     1201 acattgcctt caagctaaac aaccaaatca gagaaaatgc agaagtctcc atggacgttt
     1261 ccctggctta ccgtgatgac gcatttgctg agtggactga aatggcccat gaaagagtac
     1321 cacggaaact caaatgcacc ttcacatctc ccaagactcc agagcatgag ggccgttact
     1381 atgaatgtga tgtccttcct ttcatggaaa ttgggtctgt ggcccataag ttttaccttt
     1441 taaacatccg gctgcctgtg aatgagaaga agaaaatcaa tgtgggaatt ggggagataa
     1501 aggatatccg gttggtgggg atccaccaaa atggaggctt caccaaggtg tggtttgcca
     1561 tgaagacctt ccttacgccc agcatcttca tcattatggt gtggtattgg aggaggatca
     1621 ccatgatgtc ccgaccccca gtgcttctgg aaaaagtcat ctttgccctt gggatttcca
     1681 tgacctttat caatatccca gtggaatggt tttccatcgg gtttgactgg acctggatgc
     1741 tgctgtttgg tgacatccga cagggcatct tctatgcgat gcttctgtcc ttctggatca
     1801 tcttctgtgg cgagcacatg atggatcagc acgagcggaa ccacatcgca gggtattgga
     1861 agcaagtcgg acccattgcc gttggctcct tctgcctctt catatttgac atgtgtgaga
     1921 gaggggtaca actcacgaat cccttctaca gtatctggac tacagacatt ggaacagagc
     1981 tggccatggc cttcatcatc gtggctggaa tctgcctctg cctctgcttc ctgtttctat
     2041 gcttcatggt atttcaggtg tttcggaaca tcagtgggaa gcagtccagc ctgccagcta
     2101 tgagcaaagt ccggcggcta cactatgagg ggctaatttt taggttcaag ttcctcatgc
     2161 ttatcacctt ggcctgcgct gccatgactg tcatcttctt catcgttagt caggtaacgg
     2221 aaggccattg gaaatggggc ggcgtcacag tccaagtgaa cagtgccttt ttcacaggca
     2281 tctatgggat gtggaatctg tatgtctttg ctctgatgtt cttgtatgca ccatcccata
     2341 aaaactatgg agaagaccag tccaatggcg atctgggtgt ccatagtggg gaagaactcc
     2401 agctcaccac cactatcacc catgtggacg gacccactga gatctacaag ttgacccgca
     2461 aggaggccca ggagggtggc ggaggtggcg gttatcctta cgacgtgcct gactacgcct
     2521 aggaattctg cagatatcca gcacagtggc ggccgctcga ggttaacgac cctccccccc
     2581 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
     2641 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
     2701 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
     2761 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
     2821 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
     2881 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
     2941 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
     3001 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
     3061 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     3121 aaaaacacga tgataatatg gccacaacca tggtgagcaa gggcgaggag ctgttcaccg
     3181 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     3241 ctggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     3301 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     3361 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     3421 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     3481 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     3541 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     3601 tctatatcat ggccgacaag cagaagaacg gcatcaaggc gaacttcaag atccgccaca
     3661 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     3721 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     3781 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     3841 ctctcggcat ggacgagctg tacaagtaat gaattaatta agaattatca ccgcttctat
     3901 tcagccagta aggcctgtct ttaatggcct ccggcatgag acactttcta gagtcgagtc
     3961 tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     4021 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     4081 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     4141 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     4201 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     4261 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     4321 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     4381 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     4441 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     4501 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     4561 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     4621 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     4681 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     4741 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     4801 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     4861 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     4921 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     4981 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     5041 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt
     5101 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     5161 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     5221 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     5281 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     5341 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     5401 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     5461 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     5521 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     5581 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     5641 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     5701 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     5761 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     5821 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     5881 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     5941 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     6001 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     6061 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     6121 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     6181 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     6241 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     6301 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     6361 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     6421 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     6481 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     6541 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     6601 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     6661 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6721 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     6781 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     6841 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     6901 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     6961 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7021 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7081 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7141 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7201 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7261 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7321 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7381 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     7441 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     7501 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     7561 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     7621 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     7681 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     7741 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     7801 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     7861 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     7921 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     7981 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     8041 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     8101 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     8161 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8221 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     8281 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8341 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     8401 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctc
//
