LOCUS       sequence-351736-        8460 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of WLS-HA protein (Y478C mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8460)
  AUTHORS   Chai G, Szenker-Ravi E, Chung C, Li Z, Wang L, Khatoo M, Marshall T,
            Jiang N, Yang X, McEvoy-Venneri J, Stanley V, Anzenberg P, Lang N, 
            Wazny V, Yu J, Virshup DM, Nygaard R, Mancia F, Merdzanic R, 
            Toralles MBP, Pitanga PML, Puri RD, Hernan R, Chung WK, 
            Bertoli-Avella AM, Al-Sannaa N, Zaki MS, Willert K, Reversade B, 
            Gleeson JG
  TITLE     A Human Pleiotropic Multiorgan Condition Caused by Deficient Wnt 
            Secretion.
  JOURNAL   N Engl J Med. 2021 Sep 30;385(14):1292-1301. doi: 
            10.1056/NEJMoa2033911.
  PUBMED    34587386
REFERENCE   2  (bases 1 to 8460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "N Engl J Med"; date: "2021-09-30- 30"; volume: "385"; issue: "14"; 
            pages: "1292-1301"
COMMENT     Sequence Label: pcDNA3-Y478C-WLS-HA-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..8460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    17..569
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(161..178)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     388..407
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(581..583,584..586,587..1300)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(626..647)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(887..906)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1234..1255
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1421..1438)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1427..1651
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1697..2125
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1784..1803)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1994..2015
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2134..2154)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2139..2468
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2319..2454
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2381..2400
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2535..3329
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2589..2608)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3199..3218
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3503..3624
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3540..3559)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3594..3613
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3673..3689)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3673..3689)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3686..3708)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3697..3713
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3721..3727,3728..3745,3746..3751))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3766..3787
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3904..3921)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4075..4663)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4155..4174)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4834..5625,5626..5694))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5457..5476
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5695..5799)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5874..5893)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6065..6444
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6445..6648
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6599..6619
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6693..6712
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6693..6711
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      6731..6740
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8378..8404
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cccccccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
       61 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
      121 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
      181 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
      241 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
      301 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
      361 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
      421 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
      481 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
      541 gttttccttt gaaaaacacg atgataatat ggccacaacc atggtgagca agggcgagga
      601 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
      661 gttcagcgtg tctggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
      721 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
      781 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
      841 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
      901 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
      961 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     1021 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg cgaacttcaa
     1081 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     1141 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     1201 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     1261 cgccgggatc actctcggca tggacgagct gtacaagtaa tgaattaatt aagaattatc
     1321 accgcttcta ttcagccagt aaggcctgtc tttaatggcc tccggcatga gacactttct
     1381 agagtcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     1441 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     1501 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     1561 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     1621 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     1681 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     1741 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     1801 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     1861 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     1921 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     1981 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     2041 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     2101 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     2161 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     2221 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     2281 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     2341 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     2401 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     2461 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat
     2521 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     2581 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     2641 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     2701 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     2761 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     2821 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     2881 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     2941 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     3001 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     3061 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     3121 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     3181 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     3241 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     3301 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     3361 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     3421 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     3481 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     3541 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     3601 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     3661 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     3721 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     3781 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     3841 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     3901 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     3961 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     4021 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     4081 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     4141 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     4201 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     4261 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     4321 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     4381 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     4441 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     4501 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     4561 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     4621 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     4681 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     4741 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     4801 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     4861 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     4921 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     4981 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     5041 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     5101 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     5161 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     5221 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     5281 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     5341 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     5401 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     5461 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     5521 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     5581 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     5641 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     5701 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     5761 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     5821 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc
     5881 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
     5941 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
     6001 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg
     6061 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     6121 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     6181 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     6241 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     6301 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     6361 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     6421 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     6481 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     6541 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     6601 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact
     6661 agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa
     6721 gctggctagc gccaccatgg ctggggcaat tatagaaaac atgagcacca agaagctgtg
     6781 cattgttggt gggattctgc tcgtgttcca aatcatcgcc tttctggtgg gaggcttgat
     6841 tgctccaggg cccacaacgg cagtgtccta catgtcggtg aaatgtgtgg atgcccgtaa
     6901 gaaccatcac aagacaaaat ggttcgtgcc ttggggaccc aatcattgtg acaagatccg
     6961 agacattgaa gaggcaattc caagggaaat tgaagccaat gacatcgtgt tttctgttca
     7021 cattcccctc ccccacatgg agatgagtcc ttggttccaa ttcatgctgt ttatcctgca
     7081 gctggacatt gccttcaagc taaacaacca aatcagagaa aatgcagaag tctccatgga
     7141 cgtttccctg gcttaccgtg atgacgcatt tgctgagtgg actgaaatgg cccatgaaag
     7201 agtaccacgg aaactcaaat gcaccttcac atctcccaag actccagagc atgagggccg
     7261 ttactatgaa tgtgatgtcc ttcctttcat ggaaattggg tctgtggccc ataagtttta
     7321 ccttttaaac atccggctgc ctgtgaatga gaagaagaaa atcaatgtgg gaattgggga
     7381 gataaaggat atccggttgg tggggatcca ccaaaatgga ggcttcacca aggtgtggtt
     7441 tgccatgaag accttcctta cgcccagcat cttcatcatt atggtgtggt attggaggag
     7501 gatcaccatg atgtcccgac ccccagtgct tctggaaaaa gtcatctttg cccttgggat
     7561 ttccatgacc tttatcaata tcccagtgga atggttttcc atcgggtttg actggacctg
     7621 gatgctgctg tttggtgaca tccgacaggg catcttctat gcgatgcttc tgtccttctg
     7681 gatcatcttc tgtggcgagc acatgatgga tcagcacgag cggaaccaca tcgcagggta
     7741 ttggaagcaa gtcggaccca ttgccgttgg ctccttctgc ctcttcatat ttgacatgtg
     7801 tgagagaggg gtacaactca cgaatccctt ctacagtatc tggactacag acattggaac
     7861 agagctggcc atggccttca tcatcgtggc tggaatctgc ctctgcctct acttcctgtt
     7921 tctatgcttc atggtatttc aggtgtttcg gaacatcagt gggaagcagt ccagcctgcc
     7981 agctatgagc aaagtccggc ggctacacta tgaggggcta atttttaggt tcaagttcct
     8041 catgcttatc accttggcct gcgctgccat gactgtcatc ttcttcatcg ttagtcaggt
     8101 aacggaaggc cattggaaat ggggcggcgt cacagtccaa gtgaacagtg cctttttcac
     8161 aggcatctgt gggatgtgga atctgtatgt ctttgctctg atgttcttgt atgcaccatc
     8221 ccataaaaac tatggagaag accagtccaa tggcgatctg ggtgtccata gtggggaaga
     8281 actccagctc accaccacta tcacccatgt ggacggaccc actgagatct acaagttgac
     8341 ccgcaaggag gcccaggagg gtggcggagg tggcggttat ccttacgacg tgcctgacta
     8401 cgcctaggaa ttctgcagat atccagcaca gtggcggccg ctcgaggtta acgaattccg
//
