LOCUS       sequence-351741-       10663 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of  V5-tagged mouse Lmnb2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10663)
  TITLE     Gerace lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV V5-lamin B2
FEATURES             Location/Qualifiers
     source          1..10663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2221..2432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2433..3371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3328..3348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3387..3396
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3396..3437
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5457..6006
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5601..5618)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5828..5847
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6009..6608
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6009..6028)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6505..6525
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6632..7220
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6685..6705)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7103..7114)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7427..8060
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8188..8204)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8188..8204)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8201..8223)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8212..8228
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8236..8242,8243..8260,8261..8266))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8281..8302
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8419..8436)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8590..9178)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8670..8689)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9349..10140,10141..10209))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9972..9991
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10210..10314)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10362..10496
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10399..10418)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10453..10472
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccgccg ccatgggtaa gcctatccct aaccctctcc
     3421 tcggtctcga ttctacgggg ggcggagggg gtgggcccat gagcgcgccg cattcgggca
     3481 accgaggtga ttctcccgcc gccgccacct ccgccatggc gtctctgccg ccccacgcgg
     3541 ggcccgccac gccgctgtcg cccacgcgtc tgtctcggct gcaggagaag gaggagctcc
     3601 gtgagctcaa cgaccgcctg gcacactaca tcgaccgtgt ccgcgcgctg gagctggaga
     3661 atgataggtt gctgctccgg atctccgaga aggaggaggt gaccactcgc gaggtgagtg
     3721 gcatcaagac cctgtacgag tcagagctgg ctgacgcccg acgggtactg gatgagacgg
     3781 cccgtgaacg tgcccggctg cagattgaaa ttggaaaggt gcaggctgag ctagaggagg
     3841 ccaggaagag tgccaagaag cgggaaggtg agctcacagt ggcccagggc cgagtgaagg
     3901 atctggaatc actgtttcac cggagtgagg ctgagctggc cacagccctc agtgacaagc
     3961 agggcctgga gacagaggtg gcagagcttc gagcacagct ggccaaggca gaagatggtc
     4021 atgctgtggc caagaagcag ttggagaagg agacgctgat gcgtgtggac ctggagaacc
     4081 gatgccagag cctgcaggag gagctggctt tcagcaagag tgtgtttgag gaggaggtac
     4141 gggagacccg acggaggcac gagcggcgct tggtggaggt ggacagcagc cggcaacagg
     4201 aatatgactt caagatggct caggccctgg aggacctgcg cagtcagcac gatgagcaag
     4261 tgcgcctgta ccgggtagaa ctggagcaga cctaccaggc caagctggac aacgccaagc
     4321 tgctctcgga ccagaatgac aaggcagccc atgcagcccg cgaggagctc aaggaggccc
     4381 gcatgcgcgt ggagtccctc agctaccagc ttttaggcct ccaaaagcag gccagtgctg
     4441 cagagaacca catccatgag ctggaggagg ccttggctgg ggagcgtgac aagttccgca
     4501 agatgctgga cgctaaggaa caggagatga cggaggtgcg ggacgccatg cagcagcagc
     4561 tggcagagta ccaagagctg ctggacatta agctggccct ggacatggag ataagcgcct
     4621 accgcaagct gctggagggc gaggaggaga ggctgaagct gtctcccagc ccttcatcac
     4681 ggatcaccat ctctcgggcc acatcgagca gcagcagcag cagcggggtt ggcatgtctg
     4741 tgggccaggg ccggggcaag cgccggcggc tggagactga ggacacctca ggctcaccca
     4801 gcagggcttc cagagtgagc agcggctccc gcctggctca gcagactgtg gccacgggtg
     4861 ttgtgaacat cgacgaggtg gacccagagg gcaggttcgt gcgccttaag aactcttcag
     4921 acaaggacca gtctttgggg aactggagga tcaagagaca ggttctggag ggtgaggaca
     4981 ttgcctacaa gttcacaccc aagtatgtcc tgcgggccgg ccagactgtc acggtgtggg
     5041 cagctggcgc aggggctacc cacagtcccc catcaaccct tgtgtggaaa agccagacca
     5101 actggggccc tggggagagc ttccgcactg ccctggtcag tgccgacggt gaggaggtgg
     5161 ccgtgaaggc tgcaaagcac tcatctgtcc aggggaggga gaacggggaa gaggaggaag
     5221 aggaagaggc agaattcggt gaagaggacc tcttccacca gcagggggac ccaaggacta
     5281 cctcaagggg ctgccgactg atgtgataat gggccgcgac gatgtttaaa cttcgaaacg
     5341 cgtgaattcc tcgagggcgg ccgctctaga gtcgacgggc cgcggtaaca attgttaact
     5401 aacttaagct agcaacggtt tccctctagc gggatcaatt ccgccccccc cccctaacgt
     5461 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     5521 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     5581 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     5641 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     5701 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     5761 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     5821 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     5881 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     5941 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
     6001 ataataccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     6061 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     6121 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     6181 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     6241 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     6301 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     6361 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     6421 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     6481 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     6541 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     6601 gtgcctgacc cgggcggggc gcgtctggaa caatcaacct ctggattaca aaatttgtga
     6661 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     6721 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     6781 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     6841 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     6901 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     6961 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     7021 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     7081 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     7141 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     7201 cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga
     7261 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     7321 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     7381 aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt
     7441 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     7501 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     7561 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
     7621 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
     7681 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
     7741 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
     7801 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
     7861 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     7921 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7981 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     8041 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     8101 tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt taccgtcgac
     8161 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     8221 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     8281 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     8341 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     8401 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     8461 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     8521 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8581 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     8641 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     8701 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     8761 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     8821 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     8881 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     8941 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     9001 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     9061 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9121 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     9181 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     9241 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     9301 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     9361 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     9421 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     9481 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     9541 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     9601 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     9661 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     9721 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     9781 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     9841 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     9901 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     9961 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    10021 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    10081 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    10141 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    10201 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    10261 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    10321 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt attgcagctt
    10381 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    10441 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact
    10501 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg
    10561 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
    10621 aaagaaattg tatttgttaa atatgtacta caaacttagt agt
//
