LOCUS       sequence-351747-        9626 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-myristoylation signaling peptide of Src and the C-terminal 
            fragment of G-protein coupled receptor kinase 2 (ct-GRK2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9626)
  AUTHORS   Alten B, Guzikowski NJ, Zurawski Z, Hamm HE, Kavalali ET
  TITLE     Presynaptic mechanisms underlying GABA(B)-receptor-mediated 
            inhibition of spontaneous neurotransmitter release.
  JOURNAL   Cell Rep. 2022 Jan 18;38(3):110255. doi: 
            10.1016/j.celrep.2021.110255.
  PUBMED    35045279
REFERENCE   2  (bases 1 to 9626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2022-01-18"; volume: "38(3):110255. doi"; pages: " 
            10.1016/j.celrep.2021.110255"
COMMENT     Sequence Label: myristoylated ct-GRK2
FEATURES             Location/Qualifiers
     source          1..9626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        162..541
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        543..741
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     696..716
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             759..939
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    986..1111
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1604..1837
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2022..2066
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2364..2481
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2546..3757
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3594..3613
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             3788..3829
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     misc_feature    4242..4830
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4295..4315)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4713..4724)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4833..4849)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4834..4850)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5355..5535
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5561..5578)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5567..5791
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5837..6265
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5924..5943)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6134..6155
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6274..6294)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6279..6608
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6459..6594
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6521..6540
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6656..6703
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6722..7096
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7226..7347
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7263..7282)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7317..7336
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7396..7412)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7396..7412)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7409..7431)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7420..7436
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7444..7450,7451..7468,7469..7474))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7489..7510
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7627..7644)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7798..8386)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7878..7897)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8557..9348,9349..9417))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9180..9199
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9418..9522)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9597..9616)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
       61 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
      121 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
      181 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      241 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      301 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      361 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      421 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      481 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      541 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      601 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      661 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      721 gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga
      781 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
      841 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
      901 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
      961 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     1021 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     1081 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     1141 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     1201 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     1261 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     1321 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     1381 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     1441 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     1501 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     1561 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     1621 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     1681 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     1741 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     1801 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     1861 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     1921 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     1981 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     2041 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     2101 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     2161 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     2221 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     2281 acagagacag atccattcga ttagtgaacg gatcggcact gcgtgcgcca attctgcaga
     2341 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
     2401 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     2461 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggtta
     2521 attaacccgt gtcggctcca gatctggcct ccgcgccggg ttttggcgcc tcccgcgggc
     2581 gcccccctcc tcacggcgag cgctgccacg tcagacgaag ggcgcagcga gcgtcctgat
     2641 ccttccgccc ggacgctcag gacagcggcc cgctgctcat aagactcggc cttagaaccc
     2701 cagtatcagc agaaggacat tttaggacgg gacttgggtg actctagggc actggttttc
     2761 tttccagaga gcggaacagg cgaggaaaag tagtcccttc tcggcgattc tgcggaggga
     2821 tctccgtggg gcggtgaacg ccgatgatta tataaggacg cgccgggtgt ggcacagcta
     2881 gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac
     2941 ttggtgagta gcgggctgct gggctggccg gggctttcgt ggccgccggg ccgctcggtg
     3001 ggacggaagc gtgtggagag accgccaagg gctgtagtct gggtccgcga gcaaggttgc
     3061 cctgaactgg gggttggggg gagcgcagca aaatggcggc tgttcccgag tcttgaatgg
     3121 aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg
     3181 caagaaccca aggtcttgag gccttcgcta atgcgggaaa gctcttattc gggtgagatg
     3241 ggctggggca ccatctgggg accctgacgt gaagtttgtc actgactgga gaactcggtt
     3301 tgtcgtctgt tgcgggggcg gcagttatgg cggtgccgtt gggcagtgca cccgtacctt
     3361 tgggagcgcg cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg
     3421 gtggggccac ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc
     3481 gggcctaggg taggctctcc tgaatcgaca ggcgccggac ctctggtgag gggagggata
     3541 agtgaggcgt cagtttcttt ggtcggtttt atgtacctat cttcttaagt agctgaagct
     3601 ccggttttga actatgcgct cggggttggc gagtgtgttt tgtgaagttt tttaggcacc
     3661 ttttgaaatg taatcatttg ggtcaatatg taattttcag tgttagacta gtaaattgtc
     3721 cgctaaattc tggccgtttt tggctttttt gttagacgaa gcttgggctg caggtcgact
     3781 ctagaccatg ggttcaagca agtccaaacc gaaggatcct agccaaagac gtgaattcgg
     3841 ccacgaggag gactacgccc tgggcaagga ctgcatcatg catggctaca tgtccaagat
     3901 gggcaacccc ttcctgaccc agtggcagcg gcggtacttc tacctgttcc ctaaccggct
     3961 cgagtggcgg ggcgagggcg aggccccgca gagcctgctg accatggagg agatccagtc
     4021 ggtggaggag acgcagatca aggagcgaaa gtgcctcctc ctcaagatcc gaggtggcaa
     4081 gcagtttgtc ctgcagtgcg atagtgaccc agagctggtg cagtggaaga aggagcttcg
     4141 agacgcctac cgcgaggccc agcagctagt gcagcgggtg cccaagatga agaacaagcc
     4201 acgctcacct tagttaacaa ttcgatatca agcttatcga taatcaacct ctggattaca
     4261 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     4321 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     4381 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     4441 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     4501 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     4561 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     4621 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     4681 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     4741 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     4801 gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga gacctagaaa
     4861 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
     4921 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
     4981 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     5041 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag
     5101 gctacttccc tgattggcag aactacacac cagggccagg gatcagatat ccactgacct
     5161 ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag
     5221 gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     5281 aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc
     5341 atccggactg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     5401 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     5461 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     5521 ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     5581 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     5641 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     5701 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     5761 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     5821 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     5881 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     5941 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     6001 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     6061 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     6121 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     6181 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     6241 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     6301 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     6361 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     6421 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     6481 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     6541 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     6601 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga
     6661 caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac
     6721 catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt
     6781 cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg
     6841 tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga
     6901 caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga
     6961 ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca
     7021 gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc
     7081 cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag
     7141 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     7201 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     7261 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     7321 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     7381 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     7441 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     7501 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     7561 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     7621 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     7681 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     7741 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     7801 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     7861 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7921 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     7981 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8041 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     8101 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     8161 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     8221 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     8281 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     8341 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     8401 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     8461 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     8521 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     8581 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     8641 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     8701 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     8761 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     8821 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     8881 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     8941 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9001 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9061 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9121 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     9181 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     9241 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     9301 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     9361 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     9421 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     9481 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     9541 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
     9601 atctgctctg atgccgcata gttaag
//
