LOCUS       sequence-35175-e         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Thaler C, Koushik SV, Blank PS, Vogel SS
  TITLE     Quantitative multiphoton spectral imaging and its use for measuring 
            resonance energy transfer.
  JOURNAL   Biophys J. 2005 Oct . 89(4):2736-49.
  PUBMED    16040744
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biophys J. 
            2005 Oct . 89(4):2736-49."
COMMENT     Sequence Label: gGamma Venus
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(348..599)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(390..411)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ataacagtta caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
       61 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcagttat
      121 ctagattaaa ggatagcaca gaaaaacttc ttctccctaa atgggttttc tgaagccgga
      181 acaggtgtca ggaggggatc ttccttggca tgcgcttcac agtaggccat caaatctgca
      241 gctgccttgg acacctttat cctatcgatg ttggcttcca tcttcagctg ttctaccagt
      301 ttcctggctt gtgctatgct ggcggtgttg ttgctggcca ttccggactt gtacagctcg
      361 tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc
      421 ttctcgttgg ggtctttgct cagcttggac tggtagctca ggtagtggtt gtcgggcagc
      481 agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgccg
      541 ccgtcctcga tgttgtggcg gatcttgaag ttggccttga tgccgttctt ctgcttgtcg
      601 gcggtgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg
      661 ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg
      721 aacttcacct cggcgcgggt ctttgtagtt gccgtcgtcc ttgaagaaga
//
