LOCUS       sequence-351756-       12877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A block for the AdenoBuilder genome assembly system. The insert 
            consolidates the regions present in pAd5-B6∆E3-GFP and pAd5-B7..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12877)
  AUTHORS   Jang Y, Bunz F
  TITLE     AdenoBuilder: A platform for the modular assembly of recombinant 
            adenoviruses.
  JOURNAL   STAR Protoc. 2022 Jan 20;3(1):101123. doi: 
            10.1016/j.xpro.2022.101123. eCollection 2022 Mar 18.
  PUBMED    35098167
REFERENCE   2  (bases 1 to 12877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "STAR 
            Protoc."; date: "2022-01-20"; volume: "3(1):101123. doi"; pages: " 
            10.1016/j.xpro.2022.101123. eCollection 2022 Mar 18"
COMMENT     Sequence Label: pAd5-B6/7 ∆E3-GFP
FEATURES             Location/Qualifiers
     source          1..12877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(13..30)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(184..772)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(264..283)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(943..1734,1735..1803))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1566..1585
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1804..1908)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1976..1994
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     2300..2319
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2300..2318
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   2374..2476
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     protein_bind    7542..7558
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7566..7582
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7566..7582
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7925..7944
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    7943..7998
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     8044..8063
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(8154..8867,8868..8870,8871..8873))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(8157..8867,8868..8870,8871..8873))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8199..8220)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8548..8567
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8807..8828
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      8870..8879
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(8903..9106)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8932..8952)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9107..9410
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        complement(join(12704..12710,12711..12728,12729..12734))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    12749..12770
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
       61 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
      121 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
      181 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
      241 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
      301 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
      361 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
      421 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
      481 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
      541 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
      601 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
      661 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
      721 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
      781 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
      841 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
      901 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
      961 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     1021 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     1081 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     1141 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     1201 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     1261 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     1321 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     1381 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     1441 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     1501 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     1561 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     1621 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     1681 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     1741 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     1801 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     1861 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     1921 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     1981 gcgtatcacg aggccgcccc tgcagccgaa ttatattatt tttgccaaat aatttttaac
     2041 aaaagctctg aagtcttctt catttaaatt cttagatgat acttcatctg gaaaattgtc
     2101 ccaattagta gcatcacgct gtgagtaagt tctaaaccat ttttttattg ttgtattatc
     2161 tctaatctta ctactcgatg agttttcggt attatctcta tttttaactt ggagcaggtt
     2221 ccattcattg tttttttcat catagtgaat aaaatcaact gctttaacac ttgtgcctga
     2281 acaccatatc catccggcgt aatacgactc actataggga gagcggccgc cagatcttcc
     2341 ggatggctcg agtttttcag caagatgttc gaacatcatc aataatatac cttattttgg
     2401 attgaagcca atatgataat gagggggtgg agtttgtgac gtggcgcggg gcgtgggaac
     2461 ggggcgggtg acgtaggttt tagggcggag taacttgtat gtgttgggaa ttgtagtttt
     2521 cttaaaatgg gaagtgacgt aacgtgggaa aacggaagtg acgatttgag gaagttgtgg
     2581 gttttttggc tttcgtttct gggcgtaggt tcgcgtgcgg ttttctgggt gttttttgtg
     2641 gactttaacc gttacgtcat tttttagtcc tatatatact cgctctgcac ttggcccttt
     2701 tttacactgt gactgattga gctggtgccg tgtcgagtgg tgttttttta ataggttttc
     2761 ttttttactg gtaaggctga ctgttatggc tgccgctgtg gaagcgctgt atgttgttct
     2821 ggagcgggag ggtgctattt tgcctaggca ggagggtttt tcaggtgttt atgtgttttt
     2881 ctctcctatt aattttgtta tacctcctat gggggctgta atgttgtctc tacgcctgcg
     2941 ggtatgtatt cccccgggct atttcggtcg ctttttagca ctgaccgatg tgaatcaacc
     3001 tgatgtgttt accgagtctt acattatgac tccggacatg accgaggagc tgtcggtggt
     3061 gctttttaat cacggtgacc agttttttta cggtcacgcc ggcatggccg tagtccgtct
     3121 tatgcttata agggttgttt ttcctgttgt aagacaggct tctaatgttt aaatgttttt
     3181 ttgttatttt attttgtgtt tatgcagaaa cccgcagaca tgtttgagag aaaaatggtg
     3241 tctttttctg tggtggttcc ggagcttacc tgcctttatc tgcatgagca tgactacgat
     3301 gtgctttctt ttttgcgcga ggctttgcct gattttttga gcagcacctt gcattttata
     3361 tcgccgccca tgcaacaagc ttacatcggg gctacgctgg ttagcatagc tccgagtatg
     3421 cgtgtcataa tcagtgtggg ttcttttgtc atggttcctg gcggggaagt ggccgcgctg
     3481 gtccgtgcag acctgcacga ttatgttcag ctggccctgc gaagggacct acgggatcgc
     3541 ggtatttttg ttaatgttcc gcttttgaat cttatacagg tctgtgagga acctgaattt
     3601 ttgcaatcat gattcgctgc ttgaggctga aggtggaggg cgctctggag cagattttta
     3661 caatggccgg acttaatatt cgggatttgc ttagagatat attgagaagg tggcgagatg
     3721 agaattattt gggcatggtt gaaggtgctg gaatgtttat agaggagatt caccctgaag
     3781 ggtttagcct ttacgtccac ttggacgtga gggccgtttg ccttttggaa gccattgtgc
     3841 aacatcttac aaatgccatt atctgttctt tggctgtaga gtttgaccac gccaccggag
     3901 gggagcgcgt tcacttaata gatcttcatt ttgaggtttt ggataatctt ttggaataaa
     3961 aaaaaaaaac atggttcttc cagctcttcc cgctcctccc gtgtgtgact cgcagaacga
     4021 atgtgtaggt tggctgggtg tggcttattc tgcggtggtg gatgttatca gggcagcggc
     4081 gcatgaagga gtttacatag aacccgaagc cagggggcgc ctggatgctt tgagagagtg
     4141 gatatactac aactactaca cagagcgatc taagcggcga gaccggagac gcagatctgt
     4201 ttgtcacgcc cgcacctggt tttgcttcag gaaatatgac tacgtccggc gttccatttg
     4261 gcatgacact acgaccaaca cgatctcggt tgtctcggcg cactccgtac agtagggatc
     4321 gtctacctcc ttttgagaca gaaacccgcg ctaccatact ggaggatcat ccgctgctgc
     4381 ccgaatgtaa cactttgaca atgcacaacg tgagttacgt gcgaggtctt ccctgcagtg
     4441 tgggatttac gctgattcag gaatgggttg ttccctggga tatggttcta acgcgggagg
     4501 agcttgtaat cctgaggaag tgtatgcacg tgtgcctgtg ttgtgccaac attgatatca
     4561 tgacgagcat gatgatccat ggttacgagt cctgggctct ccactgtcat tgttccagtc
     4621 ccggttccct gcagtgtata gccggcgggc aggttttggc cagctggttt aggatggtgg
     4681 tggatggcgc catgtttaat cagaggttta tatggtaccg ggaggtggtg aattacaaca
     4741 tgccaaaaga ggtaatgttt atgtccagcg tgtttatgag gggtcgccac ttaatctacc
     4801 tgcgcttgtg gtatgatggc cacgtgggtt ctgtggtccc cgccatgagc tttggataca
     4861 gcgccttgca ctgtgggatt ttgaacaata ttgtggtgct gtgctgcagt tactgtgctg
     4921 atttaagtga gatcagggtg cgctgctgtg cccggaggac aaggcgcctt atgctgcggg
     4981 cggtgcgaat catcgctgag gagaccactg ccatgttgta ttcctgcagg acggagcggc
     5041 ggcggcagca gtttattcgc gcgctgctgc agcaccaccg ccctatcctg atgcacgatt
     5101 atgactctac ccccatgtag gcgtggactt ctccttcgcc gcccgttaag caaccgcaag
     5161 ttggacagca gcctgtggct cagcagctgg acagcgacat gaacttaagt gagctgcccg
     5221 gggagtttat taatatcact gatgagcgtt tggctcgaca ggaaaccgtg tggaatataa
     5281 cacctaagaa tatgtctgtt acccatgata tgatgctttt taaggccagc cggggagaaa
     5341 ggactgtgta ctctgtgtgt tgggagggag gtggcaggtt gaatactagg gttctgtgag
     5401 tttgattaag gtacggtgat ctgtataagc tatgtggtgg tggggctata ctactgaatg
     5461 aaaaatgact tgaaattttc tgcaattgaa aaataaacac gttgaaacat aacacaaacg
     5521 attctttatt cttgggcaat gtatgaaaaa gtgtaagagg atgtggcaaa tatttcatta
     5581 atgtagttgt ggccagacca gtcccatgaa aatgacatag agtatgcact tggagttgtg
     5641 tctcctgttt cctgtgtacc gtttagtgta atggttagtg ttacaggttt agttttgtct
     5701 ccgtttaagt aaacttgact gacaatgtta cttttggcag ttttaccgtg agattttgga
     5761 taagctgata ggttaggcat aaatccaaca gcgtttgtat aggctgtgcc ttcagtaaga
     5821 tctccatttc taaagttcca atattctggg tccaggaagg aattgtttag tagcactcca
     5881 ttttcgtcaa atcttataat aagatgagca ctttgaactg ttccagatat tggagccaaa
     5941 ctgcctttaa cagccaaaac tgaaactgta gcaagtattt gactgccaca ttttgttaag
     6001 accaaagtga gtttagcatc tttctctgca tttagtctac agttaggaga tggagctggt
     6061 gtggtccaca aagttagctt atcattattt ttgtttccta ctgtaatggc acctgtgctg
     6121 tcaaaactaa ggccagttcc tagtttagga accatagcct tgtttgaatc aaattctagg
     6181 ccatggccaa tttttgtttt gaggggattt gtgtttggtg cattaggtga accaaattca
     6241 agcccatctc ctgcattaat ggctatggct gtagcgtcaa acatcaaccc cttggcagtg
     6301 cttaggttaa cctcaagctt tttggaattg tttgaagctg taaacaagta aaggcctttg
     6361 ttgtagttaa tatccaagtt gtgggctgag tttataaaaa gagggccctg tcctagtctt
     6421 agatttagtt ggttttgagc atcaaacgga taactaacat caagtataag gcgtctgttt
     6481 tgagaatcaa tccttagtcc tcctgctaca ttaagttgca tattgccttg tgaatcaaaa
     6541 cccaaggctc cagtaacttt agtttgcaag gaagtattat taatagtcac acctggacca
     6601 gttgctacgg tcaaagtgtt taggtcgtct gttacatgca aaggagcccc gtactttagt
     6661 cctagttttc cattttgtgt ataaatgggc tctttcaagt caatgcccaa gctaccagtg
     6721 gcagtagtta gagggggtga ggcagtgata gtaagggtac tgctatcggt ggtggtgagg
     6781 gggcctgatg tttgcagggc tagctttcct tctgacactg tgaggggtcc ttgggtggca
     6841 atgctaagtt tggagtcgtg cacggttagc ggggcctgtg attgcatggt gagtgtgttg
     6901 cccgcgacca ttagaggtgc ggcggcagcc acagttaggg cttctgaggt aactgtgagg
     6961 ggtgcagata tttccaggtt tatgtttgac ttggtttttt tgagaggtgg gctcacagtg
     7021 gttacatttt gggaggtaag gttgccggcc tcgtccagag agaggccgtt gcccattttg
     7081 agcgcaagca tgccattgga ggtaactaga ggttcggata ggcgcaaaga gagtacccca
     7141 gggggactct cttgaaaccc attgggggat acaaagggag gagtaagaaa aggcacagtt
     7201 ggaggaccgg tttccgtgtc atatggatac acggggttga aggtatcttc agacggtctt
     7261 gcgcgcttca tctgcaacaa catgaagata gtgggtgcgg atggacagga acaggaggaa
     7321 actgacattc catttagatt gtggagaaag tttgcagcca ggaggaagct gcaataccag
     7381 agctgggagg agggcaagga ggtgctgctg aataaactgg acagaaattt gctaactgat
     7441 tttaagtaag tgatgcttta ttattttttt ttattagtta aagggaataa gatctttgag
     7501 accgtcgaca tatggatccc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     7561 tcacacagga aacagctatg accatgatta cgccaagctt gggctgcagg tcgagggatc
     7621 tccataagag aagagggaca gctatgactg ggagtagtca ggagaggagg aaaaatctgg
     7681 ctagtaaaac atgtaaggaa aattttaggg atgttaaaga aaaaaataac acaaaacaaa
     7741 atataaaaaa aatctaacct caagtcaagg cttttctatg gaataaggaa tggacagcag
     7801 ggggctgttt catatactga tgacctcttt atagccaacc tttgttcatg gcagccagca
     7861 tatgggcata tgttgccaaa ctctaaacca aatactcatt ctgatgtttt aaatgatttg
     7921 ccctcccata tgtccttccg agtgagagac acaaaaaatt ccaacacact attgcaatga
     7981 aaataaattt cctttattag ccagaagtca gatgctcaag gggcttcatg atgtccccat
     8041 aatttttggc agagggaaaa agatcgatct cagtggtatt tgtgagccag ggcattggcc
     8101 acaccagcca ccaccttctg ataggcagcc tgcacctgag gagtgcggcc gctttacttg
     8161 tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg
     8221 tgatcgcgct tctcgttggg gtctttgctc agggcggact gggtgctcag gtagtggttg
     8281 tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc
     8341 tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat gccgttcttc
     8401 tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc
     8461 aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg
     8521 tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg
     8581 gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg
     8641 tggtcggggt agcggctgaa gcactgcacg ccgtaggtca gggtggtcac gagggtgggc
     8701 cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg
     8761 gcatcgccct cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc
     8821 tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac catggtggcg
     8881 ggatctgacg gttcactaaa ccagctctgc ttatatagac ctcccaccgt acacgcctac
     8941 cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt
     9001 ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt
     9061 caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga
     9121 tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat
     9181 aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat
     9241 acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat
     9301 ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg
     9361 ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca
     9421 tatatgggct atgaactaat gaccccgaga tccttcagtc gtagccgtcc gccgagtcct
     9481 ttaccgcgtc aaagttagga ataaattgat ccggatagtg gccgggaggt cccgagaagg
     9541 ggttaaagta gaccgatggc acaaactcct caataaattg cagagttcca atgcctccag
     9601 agcgcggctc agaggacgag gtctgcagag ttaggattgc ctgacgaggc gtgaatgaag
     9661 agcggccggc gccgccgatc tgaaatgtcc cgtccggacg gagaccaagc gaggagctca
     9721 ccgactcgtc gttgagctga atacctcgcc ctctgattgt caggtgagtt ataccctgcc
     9781 cgggcgaccg caccctgtga cgaaagccgc ccgcaagctg cgcccctgag ttagtcatct
     9841 gaacttcggc ctgggcgtct ctgggaagta ccacagtggt gggagcggga ctttcctggt
     9901 acaccagggc agcgggccaa ctacggggat taaggttatt acgaggtgtg gtggtaatag
     9961 ccgcctgttc caggagaatt cggtttcggt gggcgcgtat tccgttgacc cgggatatca
    10021 tgtggggtcc cgcgctcatg tagtttattc gggttgagta gtcttgggca gctccagccg
    10081 caagtcccat ttgtggctgg taactccaca tgtagggcgt gggaatttcc ttgctcataa
    10141 tggcgctgac aacaggtgct ggcgccgggt gtggccgctg gagatgacgt agttttcgcg
    10201 cttaaatttg agaaagggcg cgaaactagt ccttaagagt cagcgcgcag tatttactga
    10261 agagagcctc cgcgtcttcc agcgtgcgcc gaagctgatc ttcgcttttg tgatacaggc
    10321 agctgcgggt gagggatcgc agagacctgt tttttatttt cagctcttgt tcttggcccc
    10381 tgctctgttg aaatatagca tacagagtgg gaaaaatcct gtttctaagc tcgcgggtcg
    10441 atacgggttc gttgggcgcc agacgcagcg ctcctcctcc tgctgctgcc gccgctgtgg
    10501 atttcttggg ctttgtcaga gtcttgctat ccggtcgcct ttgcttctgt gtggccgctg
    10561 ctgttgctgc cgctgccgcc ggtgcagtat gggctgtaga gatgacggta gtaatgcagg
    10621 atgttacggg ggaaggccac gccgtgatgg tagagaagaa agcggcgggc gaaggagatg
    10681 ttgcccccac agtcttgcaa gcaagcaact atggcgttct tgtgcccgcg ccatgagcgg
    10741 tagccttggc gctgttgttg ctcttgggct aacggcggcg gctgcttgga cttaccggcc
    10801 ctggttccag tggtgtccca tctacggttg ggtcggcgaa cgggcagtgc cggcggcgcc
    10861 tgaggagcgg aggttgtagc catgctggaa ccggttgccg atttctgggg cgccggcgag
    10921 gggaatgcga ccgagggtga cggtgtttcg tctgacacct cttcgacctc ggaagcttcc
    10981 tcgtctaggc tctcccagtc ttccatcatg tcctcctcct cctcgtccaa aacctcctct
    11041 gcctgactgt cccagtattc ctcctcgtcc gtgggtggcg gcggcagctg cagcttcttt
    11101 ttgggtgcca tcctgggaag caagggcccg cggctgctgc tgatagggct gcggcggcgg
    11161 ggggattggg ttgagctcct cgccggactg ggggtccaag taaacccccc gtccctttcg
    11221 tagcagaaac tcttggcggg ctttgttgat ggcttgcaat tggccaagaa tgtggccctg
    11281 ggtaatgacg caggcggtaa gctccgcatt aggcgggcgg gattggtctt cgtagaacct
    11341 aatctcgtgg gcgtggtagt cctcaggtac aaatttgcga aggtaagccg acgtccacag
    11401 ccccggagtg agtttcaacc ccggagccgc ggacttttcg tcaggcgagg gaccctgcag
    11461 ctcaaaggta ccgataattt gactttcgtt aagcagctgc gaattgcaaa ccagggagcg
    11521 gtgcggggtg cataggttgc agcgacagtg acactccagt agaccgtcac cgctcacgtc
    11581 ttccattatg tcagagtggt aggcaaggta gttggctagc tgcagaaggt agcagtggcc
    11641 ccaaagcggc ggagggcatt cgcggtactt aatgggcaca aagtcgctag gaagtgcaca
    11701 gcaggtggcg ggcaagattc ctgagcgctc taggataaag ttcctaaagt tctgcaacat
    11761 gctttgactg gtgaagtctg gcagaccctg ttgcagggtt ttaagcaggc gttcggggaa
    11821 aatgatgtcc gccaggtgcg cggccacgga gcgctcgttg aaggccgtcc ataggtcctt
    11881 caagttttgc tttagcagtt tctgcagctc cttgaggttg cactcctcca agcactgctg
    11941 ccaaacgccc atggccgtct gccaggtgta gcatagaaat aagtaaacgc agtcgcggac
    12001 gtagtcgcgg cgcgcctcgc ccttgagcgt ggaatgaagc acgttttgcc caaggcggtt
    12061 ttcgtgcaaa attccaaggt aggagaccag gttgcagagc tccacgttgg agatcttgca
    12121 ggcctggcgt acgtagccct gtcgaaaggt gtagtgcaat gtttcctcta gcttgcgctg
    12181 catctccggg tcagcaaaga accgctgcat gcactcaagc tccacggtaa cgagcactgc
    12241 ggccatcatt agtttgcgtc gcttcgaaga tctttctaga agatctccta caatattctc
    12301 agctgccatg gaaaatcgat gttcttcttt tattctctca agattttcag gctgtatatt
    12361 aaaacttata ttaagaacta tgctaaccac ctcatcagga accgttgtag gtggcgtggg
    12421 ttttcttggc aatcgactct catgaaaact acgagctaaa tattcaatat gttcctcttg
    12481 accaacttta ttctgcattt tttttgaacg aggtttagag caagcttcag gaaactgaga
    12541 caggaatttt attaaaaatt taaattttga agaaagttca gggttaatag catccatttt
    12601 ttgctttgca agttcctcag cattcttaac aaaagacgtc tcttttgaca tgtttaaagt
    12661 ttaaacctcc tgtgtgaaat tattatccgc tcataattcc acacattata cgagccggaa
    12721 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
    12781 gctcactgcc aattgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
    12841 gccaacgcgc ggggagaggc ggtttgcgta ttgggcg
//
