LOCUS       sequence-351759-        5670 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  psbEFLJ integrative plasmid expresses CRE recombinase from the rbcL 
            promoter uses zeocin to integrate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Jones CM, Parrish S, Nielsen DR
  TITLE     Exploiting Polyploidy for Markerless and Plasmid-Free Genome 
            Engineering in Cyanobacteria.
  JOURNAL   ACS Synth Biol. 2021 Sep 17;10(9):2371-2382. doi: 
            10.1021/acssynbio.1c00269. Epub 2021 Aug 17.
  PUBMED    34530614
REFERENCE   2  (bases 1 to 5670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00269"; journalName: "ACS Synth Biol"; date: 
            "2021-09-17- 17"; volume: "10"; issue: "9"; pages: "2371-2382"
COMMENT     Sequence Label: pCJ155
FEATURES             Location/Qualifiers
     source          1..5670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(648..1193)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(728..747)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(3583..3601)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3597..3644
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        3676..3723
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        4915..5019
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5020..5088,5089..5670,1..210)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5238..5257)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
       61 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
      121 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
      181 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
      241 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
      301 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
      361 gaaaagatca aaggatcttc gcgctagcgg agtgtatact ggcttactat gttggcactg
      421 atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa aaggctgcac cggtgcgtca
      481 gcagaatatg tgatacagga tatattccgc ttcctcgctc actgactcgc tacgctcggt
      541 cgttcgactg cggcgagcgg aaatggctta cgaacggggc ggagatttcc tggaagatgc
      601 caggaagata cttaacaggg aagtgagagg gccgcggcaa agccgttttt ccataggctc
      661 cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg aaacccgaca
      721 ggactataaa gataccaggc gtttccccct ggcggctccc tcgtgcgctc tcctgttcct
      781 gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttgtctca ttccacgcct
      841 gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg aaccccccgt
      901 tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggaaagaca
      961 tgcaaaagca ccactggcag cagccactgg taattgattt agaggagtta gtcttgaagt
     1021 catgcgccgg ttaaggctaa actgaaagga caagttttgg tgactgcgct cctccaagcc
     1081 agttacctcg gttcaaagag ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg
     1141 cggttttttc gttttcagag caagagatta cgcgcagacc aaaacgatct caagaagatc
     1201 atcttattaa tcagataaaa tatttctaga tttcagtgca atttatctct tcaaatgtag
     1261 cacctgaagt cagccccata cgatataagt tgtaaacgcc agcaacgcgg cctttttacg
     1321 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     1381 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     1441 cgagcgcagc gagtcagtga gcgaggaagc ggaagagccc cataaccgga attcttcccg
     1501 tcgcctccaa agcatgggct ttaatggcga agataagctc tggttgttag cccgtggggg
     1561 agttgtgcaa tttagcgacg atacaaatcc agacaatgca gaagcctgga gcgaaccagt
     1621 gacaccgcag taccgcaaca gtgttggttt gttgcacatt ggctatcgta cccccgcaga
     1681 actatgggcc gtcggtggta gtggcagtgt tgttgtgagt aaagatggtg gcgacacttg
     1741 gttccgggac gctgccctag aagagatccc gacaaacttt taccgagtcg ttttcttaaa
     1801 tgagaataaa ggcttcatcc ttgggcaaca gggggtgatt ttacggtacg atacatccac
     1861 ggaagcggcc taagggcaac ccattctatt gtgggagtgt ctgttaattc cgtatgatat
     1921 aggctaattt gtttaataag ttttttttca ttaacaattg agaggagaat agtcgccccc
     1981 acaaaacttt catgattgct tgagtgaaaa ttaaatgttt aaagttctta aaggagattg
     2041 ttgagaaaca taaatactta attcatctca tttgaacgct ttccctctct aaagatcccg
     2101 acagaaaacg gttttagccc aatgtctcat taggtagcat ggctgaattc gagcgggatt
     2161 ttatggcttt tttaggtatt tttgtaaggg taaaataggc ccatcaaaca gcattagaaa
     2221 tgctaatcag cccaaaaaac aaaagcaatc tttttttgtt gctaaaagat aaaaataagt
     2281 cgaggctgtg gtaacatatc ccacagatta aagaaagtca taagacttga atcttcagaa
     2341 ttttaaaaag cagttttgcc aacgtaagat ttttgaagtt ttcgaccaac aataccgtta
     2401 ctggtatttg tctgttaaag ataagcattt ttgctggagg aaaaccgcat ggttcagacc
     2461 aaatctgctg ggtttaatgc cggttccaac ctgctgacgg tgcaccagaa tctgccggca
     2521 ctgccggtcg atgcaaccag tgacgaagtg cgcaaaaatc tgatggatat gtttcgtgac
     2581 cgccaagcct tcagcgaaca tacgtggaaa atgctgctgt cggtttgccg tagctgggcg
     2641 gcctggtgta aactgaacaa tcgcaaatgg tttccggcag aaccggaaga tgtgcgtgac
     2701 tatctgctgt acctgcaggc acgtggtctg gcagttaaaa ccatccagca acatctgggc
     2761 caactgaaca tgctgcaccg tcgctctggt ctgccgcgtc cgagtgattc caatgccgtc
     2821 agtctggtga tgcgtcgcat tcgtaaagaa aacgtggatg caggcgaacg cgctaaacag
     2881 gcactggctt ttgaacgtac cgatttcgac caagttcgct ctctgatgga aaacagtgat
     2941 cgttgccagg acatccgcaa tctggcattc ctgggtattg cttataacac cctgctgcgc
     3001 attgcagaaa tcgctcgtat tcgcgtgaaa gatatcagcc gtacggacgg cggtcgcatg
     3061 ctgattcaca tcggccgtac caaaacgctg gtttccaccg cgggcgtcga aaaagccctg
     3121 tcactgggtg tcacgaaact ggtggaacgc tggatttcag tttcgggcgt cgcagatgac
     3181 ccgaacaatt acctgttttg tcgtgtgcgc aaaaatggtg ttgcagctcc gagcgctacc
     3241 tctcagctga gtacgcgtgc gctggaaggc atcttcgaag ccacccatcg cctgatttat
     3301 ggcgcgaaag atgacagcgg tcagcgttac ctggcatggt ccggtcactc agctcgtgtt
     3361 ggtgcagcac gtgatatggc acgtgcaggt gtctctatcc cggaaattat gcaggccggc
     3421 ggttggacga acgtgaatat tgttatgaac tatattcgta acctggactc tgaaacgggt
     3481 gcgatggtgc gtctgctgga agatggcgac tgacgagggc ggtgctttgg caggatccgg
     3541 ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag
     3601 cataacccct tggggcctct aaacgggtct tgacgggttt tttgtctaga tcaacggcct
     3661 caacctacta ctggggttga caattaatca tcggcatagt atatcggcat agtataatac
     3721 gacaaggtga ggaactaaac catgaagctg accagcgccg ttccggtgct caccgcgcgc
     3781 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg
     3841 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag
     3901 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg
     3961 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg
     4021 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac
     4081 tgcgtgcact tcgtggccga ggagcaggac taaccgacgt cgacccactc tagaggatcc
     4141 ccgctccgtg taaatggagg cgctcgttga tctgagcctt gccccctgac gaacggcggt
     4201 ggatggaaga tactgctctc aagtgctgaa gcggtagctt agctccccgt ttcgtgctga
     4261 tcagtctttt tcaacacgta aaaagcggag gagttttgca attttgttgg ttgtaacgat
     4321 cctccgttgc aattgatgca caattcgctc taattaattg ttgagacacc acctgctcta
     4381 attgaggggt ggttttgttt taggaacttc tggggcaata gggagtccaa aaaagtttgg
     4441 ggataatcgt caacggtgca gtatgactat gaaaagacag ttgcgggatc tgaaagtatg
     4501 ggtgatggtg ggtctgttga gtttaggtgg agcctggggt caaagggcga tcgccgctga
     4561 aaccctccga ctaaaattag ggccacttca acaaacttta caattagaag atttagaaac
     4621 ctttgccgaa acgggtcaag tcccccgcaa cctccgaccc tatggggtat ttttggatgg
     4681 taatttgcag aaatttctcc aacgacgctt gcagatcgag ccagagatgg cggatcagtt
     4741 ttttgatcag ttgtggcgat cgcctacggg aaaacagatt ttggcgcaaa ttcaaacggc
     4801 cctaccggga acttctattc aggatctcca ggcgagatac gcctattttt ataggttaat
     4861 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     4921 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     4981 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     5041 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5101 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5161 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5221 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     5281 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5341 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     5401 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     5461 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     5521 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     5581 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     5641 tggatggagg cggataaagt tgcaggacca
//
