LOCUS       sequence-351762-        5948 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  rbcLXS6803 integrative plasmid expresses CRE recombinase as a 
            translational fusion to rbcL6803.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5948)
  AUTHORS   Jones CM, Parrish S, Nielsen DR
  TITLE     Exploiting Polyploidy for Markerless and Plasmid-Free Genome 
            Engineering in Cyanobacteria.
  JOURNAL   ACS Synth Biol. 2021 Sep 17;10(9):2371-2382. doi: 
            10.1021/acssynbio.1c00269. Epub 2021 Aug 17.
  PUBMED    34530614
REFERENCE   2  (bases 1 to 5948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00269"; journalName: "ACS Synth Biol"; date: 
            "2021-09-17- 17"; volume: "10"; issue: "9"; pages: "2371-2382"
COMMENT     Sequence Label: pZD-CJ1111
FEATURES             Location/Qualifiers
     source          1..5948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      67..655
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     556..575
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     809..826
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2796..2814)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2810..2857
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(2992..3011)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3003..3818
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             3003..3818
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3076..3095)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      complement(3869..3896)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(3912..3983)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        4879..4983
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4984..5052,5053..5844)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5202..5221)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
       61 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
      121 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
      181 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
      241 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
      301 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
      361 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
      421 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
      481 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
      541 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
      601 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
      661 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
      721 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
      781 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagaggc
      841 cgacggttat atcttggatt tgcgtaacaa ccccggtggc ttactccagg ctggtattga
      901 cattgctcgg ttgtggttac cggaaagcac cattgtctac accgttaatc gccaaggcac
      961 ccaggaaagt ttcactgcca atggagaagc ggcgaccgat cgcccgttgg tggtgttggt
     1021 caaccagggt actgccagtg ccagcgaaat tttagccgga gctttgcagg ataatcagcg
     1081 ggccactcta gtgggggaaa aaacctttgg taagggtttg attcaatcct tgtttgaact
     1141 atccgatggg gccggcattg ccgtcacggt ggccaaatac gaaacccccc aacatcacga
     1201 catccataaa ctgggcatta tgcccgatga agtggtggag caacccctga ttagctttgc
     1261 ggaaattact tcccccgccg atgtgcaata ccaagccgcc ttagatttgc tcaccggagg
     1321 agtggcaatc gcccataaat cttcttcaat tcccgccatg gcaacggctc acaagcccaa
     1381 ctaatcacca tttggacaaa acatcaggaa ttctaattag aaagtccaaa aattgtaatt
     1441 taaaaaacag tcaatggaga gcattgccat aagtaaaggc atcccctgcg tgataagatt
     1501 accttcagaa aacagatagt tgctgggtta tcgcagattt ttctcgcaac caaataactg
     1561 taaataataa ctgtctctgg ggcgacggta ggctttatat tgccaaattt cgcccgtggg
     1621 agaaagctag gctattcaat gtttatggag gactgaccta gatggtacaa gccaaagcag
     1681 ggtttaaggc gggcgtatcc aacctgctga cggtgcacca gaatctgccg gcactgccgg
     1741 tcgatgcaac cagtgacgaa gtgcgcaaaa atctgatgga tatgtttcgt gaccgccaag
     1801 ccttcagcga acatacgtgg aaaatgctgc tgtcggtttg ccgtagctgg gcggcctggt
     1861 gtaaactgaa caatcgcaaa tggtttccgg cagaaccgga agatgtgcgt gactatctgc
     1921 tgtacctgca ggcacgtggt ctggcagtta aaaccatcca gcaacatctg ggccaactga
     1981 acatgctgca ccgtcgctct ggtctgccgc gtccgagtga ttccaatgcc gtcagtctgg
     2041 tgatgcgtcg cattcgtaaa gaaaacgtgg atgcaggcga acgcgctaaa caggcactgg
     2101 cttttgaacg taccgatttc gaccaagttc gctctctgat ggaaaacagt gatcgttgcc
     2161 aggacatccg caatctggca ttcctgggta ttgcttataa caccctgctg cgcattgcag
     2221 aaatcgctcg tattcgcgtg aaagatatca gccgtacgga cggcggtcgc atgctgattc
     2281 acatcggccg taccaaaacg ctggtttcca ccgcgggcgt cgaaaaagcc ctgtcactgg
     2341 gtgtcacgaa actggtggaa cgctggattt cagtttcggg cgtcgcagat gacccgaaca
     2401 attacctgtt ttgtcgtgtg cgcaaaaatg gtgttgcagc tccgagcgct acctctcagc
     2461 tgagtacgcg tgcgctggaa ggcatcttcg aagccaccca tcgcctgatt tatggcgcga
     2521 aagatgacag cggtcagcgt tacctggcat ggtccggtca ctcagctcgt gttggtgcag
     2581 cacgtgatat ggcacgtgca ggtgtctcta tcccggaaat tatgcaggcc ggcggttgga
     2641 cgaacgtgaa tattgttatg aactatattc gtaacctgga ctctgaaacg ggtgcgatgg
     2701 tgcgtctgct ggaagatggc gactgacgag ggcggtgctt tggcaggatc cggctgctaa
     2761 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc
     2821 ccttggggcc tctaaacggg tcttgacggg ttttttgtct agatcaacgg cctcaaccta
     2881 ctactgggca acaaagccac gttgtgtctc aaaatctctg atgttacatt gcacaagata
     2941 aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg
     3001 ttatgagcca tattcaacgg gaaacgtctt gctccaggcc gcgattaaat tccaacatgg
     3061 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
     3121 tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
     3181 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
     3241 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
     3301 cgatccccgg gaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
     3361 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc
     3421 cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt
     3481 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
     3541 aagaaatgca taagcttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
     3601 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
     3661 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt
     3721 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
     3781 aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt
     3841 aacactggca gagcctctag tatataaacg cagaaaggcc cacccgaagg tgagccagtg
     3901 tgactctagt agagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc
     3961 gactgagcct ttcgttttat ttggttacag ttttggcaat tactaaaaaa ctgacttcaa
     4021 ttcaatgtta gcccgctccc gcgggttttt tgttgctttt tcacagtgac tataggtaat
     4081 cagcaacaca atacggccct gttctttgga cagtttttgt ataatgttga ccgcatcctg
     4141 accggatttt ttatctaagt ggggaattgt caattgtcaa ttaaagctaa gttctactaa
     4201 tgttttagaa ggcattgtcg attgaaaata agggttgaat ggagaaaatt ttgagccttt
     4261 gtcaaagata aaaatttatt tcaacagttt tttaactagc cgaaccagag aatgacccag
     4321 tggcgctgac tttgctcccg agtttttgtt agaaattacc ctcaagaagt aatctaataa
     4381 taaacctaac cgaataattt cccaggggag tattccggaa aaccatggtt aaacttactt
     4441 gccatccccc atggtaaaat tgcaacgatt ttgatcaaag tcctaatttt tttgtaaagc
     4501 ttttagtaat ccttctgatt ttcccatgaa gctcatcgat tcccgtggca gaattttcgg
     4561 catagtcagc ctgttggatc tgggggccgc cttgatcatc ctcatggtag ctgtgggaat
     4621 tttcgttctg ccgggcagtt ctggcaaaag cattcttgcc caagccaacg ccgcttccat
     4681 tgaattgacg accattgtcc ggggattaaa cgtattagat ccccaggtgg tgctggatga
     4741 gtttaaagcc gaaaaaacca acatcattat tcgcaatcaa ccggctggcc aggtggaggg
     4801 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     4861 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     4921 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     4981 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     5041 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     5101 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     5161 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     5221 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     5281 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     5341 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     5401 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     5461 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     5521 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     5581 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     5641 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     5701 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     5761 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     5821 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     5881 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     5941 atgaccaa
//
