LOCUS       sequence-351768-       11473 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11473)
  AUTHORS   Yarnall MTN, Ioannidi EI, Schmitt-Ulms C, Krajeski RN, Lim J, 
            Villiger L, Zhou W, Jiang K, Garushyants SK, Roberts N, Zhang L, 
            Vakulskas CA, Walker JA 2nd, Kadina AP, Zepeda AE, Holden K, Ma H, 
            Xie J, Gao G, Foquet L, Bial G, Donnelly SK, Miyata Y, Radiloff DR, 
            Henderson JM, Ujita A, Abudayyeh OO, Gootenberg JS
  TITLE     Drag-and-drop genome insertion of large sequences without 
            double-strand DNA cleavage using CRISPR-directed integrases.
  JOURNAL   Nat Biotechnol. 2022 Nov 24. doi: 10.1038/s41587-022-01527-4.
  PUBMED    36424489
REFERENCE   2  (bases 1 to 11473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Nov 24. doi: 10.1038/s41587-022-01527-4."
COMMENT     Sequence Label: pDY0863 PASTEv4
            pCMV-SpCas9-XTEN-RT(L139P)-(GG...
FEATURES             Location/Qualifiers
     source          1..11473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(70..89)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        261..640
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        641..844
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     795..815
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     886..905
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        886..904
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             954..974
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(975..3488,3489..3491,3492..5075)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             975..5075
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7344..7364
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7413..7433
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7434..7460
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(9037..9054)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9043..9267
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9338..9354)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9338..9354)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9351..9373)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9362..9378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9386..9392,9393..9410,9411..9416))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9431..9452
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9569..9586)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9740..10328)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9820..9839)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10499..11290,11291..11359))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11122..11141
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11360..11464)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccgaaaagtg ccacctgacg tcgacggatc gggagatcga tctcccgatc ccctagggtc
       61 tactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
      121 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
      181 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
      241 acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac
      301 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
      361 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
      421 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
      481 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
      541 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
      601 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
      661 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
      721 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
      781 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
      841 agctggttta gtgaaccgtc agatccgcta gagatccgcg gccgctaata cgactcacta
      901 tagggagagc cgccaccatg aaacggacag ccgacggaag cgagttcgag tcaccaaaga
      961 agaagcggaa agtcgacaag aagtacagca tcggcctgga catcggcacc aactctgtgg
     1021 gctgggccgt gatcaccgac gagtacaagg tgcccagcaa gaaattcaag gtgctgggca
     1081 acaccgaccg gcacagcatc aagaagaacc tgatcggagc cctgctgttc gacagcggcg
     1141 aaacagccga ggccacccgg ctgaagagaa ccgccagaag aagatacacc agacggaaga
     1201 accggatctg ctatctgcaa gagatcttca gcaacgagat ggccaaggtg gacgacagct
     1261 tcttccacag actggaagag tccttcctgg tggaagagga taagaagcac gagcggcacc
     1321 ccatcttcgg caacatcgtg gacgaggtgg cctaccacga gaagtacccc accatctacc
     1381 acctgagaaa gaaactggtg gacagcaccg acaaggccga cctgcggctg atctatctgg
     1441 ccctggccca catgatcaag ttccggggcc acttcctgat cgagggcgac ctgaaccccg
     1501 acaacagcga cgtggacaag ctgttcatcc agctggtgca gacctacaac cagctgttcg
     1561 aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc catcctgtct gccagactga
     1621 gcaagagcag acggctggaa aatctgatcg cccagctgcc cggcgagaag aagaatggcc
     1681 tgttcggaaa cctgattgcc ctgagcctgg gcctgacccc caacttcaag agcaacttcg
     1741 acctggccga ggatgccaaa ctgcagctga gcaaggacac ctacgacgac gacctggaca
     1801 acctgctggc ccagatcggc gaccagtacg ccgacctgtt tctggccgcc aagaacctgt
     1861 ccgacgccat cctgctgagc gacatcctga gagtgaacac cgagatcacc aaggcccccc
     1921 tgagcgcctc tatgatcaag agatacgacg agcaccacca ggacctgacc ctgctgaaag
     1981 ctctcgtgcg gcagcagctg cctgagaagt acaaagagat tttcttcgac cagagcaaga
     2041 acggctacgc cggctacatt gacggcggag ccagccagga agagttctac aagttcatca
     2101 agcccatcct ggaaaagatg gacggcaccg aggaactgct cgtgaagctg aacagagagg
     2161 acctgctgcg gaagcagcgg accttcgaca acggcagcat cccccaccag atccacctgg
     2221 gagagctgca cgccattctg cggcggcagg aagattttta cccattcctg aaggacaacc
     2281 gggaaaagat cgagaagatc ctgaccttcc gcatccccta ctacgtgggc cctctggcca
     2341 ggggaaacag cagattcgcc tggatgacca gaaagagcga ggaaaccatc accccctgga
     2401 acttcgagga agtggtggac aagggcgctt ccgcccagag cttcatcgag cggatgacca
     2461 acttcgataa gaacctgccc aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt
     2521 acttcaccgt gtataacgag ctgaccaaag tgaaatacgt gaccgaggga atgagaaagc
     2581 ccgccttcct gagcggcgag cagaaaaagg ccatcgtgga cctgctgttc aagaccaacc
     2641 ggaaagtgac cgtgaagcag ctgaaagagg actacttcaa gaaaatcgag tgcttcgact
     2701 ccgtggaaat ctccggcgtg gaagatcggt tcaacgcctc cctgggcaca taccacgatc
     2761 tgctgaaaat tatcaaggac aaggacttcc tggacaatga ggaaaacgag gacattctgg
     2821 aagatatcgt gctgaccctg acactgtttg aggacagaga gatgatcgag gaacggctga
     2881 aaacctatgc ccacctgttc gacgacaaag tgatgaagca gctgaagcgg cggagataca
     2941 ccggctgggg caggctgagc cggaagctga tcaacggcat ccgggacaag cagtccggca
     3001 agacaatcct ggatttcctg aagtccgacg gcttcgccaa cagaaacttc atgcagctga
     3061 tccacgacga cagcctgacc tttaaagagg acatccagaa agcccaggtg tccggccagg
     3121 gcgatagcct gcacgagcac attgccaatc tggccggcag ccccgccatt aagaagggca
     3181 tcctgcagac agtgaaggtg gtggacgagc tcgtgaaagt gatgggccgg cacaagcccg
     3241 agaacatcgt gatcgaaatg gccagagaga accagaccac ccagaaggga cagaagaaca
     3301 gccgcgagag aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc cagatcctga
     3361 aagaacaccc cgtggaaaac acccagctgc agaacgagaa gctgtacctg tactacctgc
     3421 agaatgggcg ggatatgtac gtggaccagg aactggacat caaccggctg tccgactacg
     3481 atgtggacgc tatcgtgcct cagagctttc tgaaggacga ctccatcgac aacaaggtgc
     3541 tgaccagaag cgacaagaac cggggcaaga gcgacaacgt gccctccgaa gaggtcgtga
     3601 agaagatgaa gaactactgg cggcagctgc tgaacgccaa gctgattacc cagagaaagt
     3661 tcgacaatct gaccaaggcc gagagaggcg gcctgagcga actggataag gccggcttca
     3721 tcaagagaca gctggtggaa acccggcaga tcacaaagca cgtggcacag atcctggact
     3781 cccggatgaa cactaagtac gacgagaatg acaagctgat ccgggaagtg aaagtgatca
     3841 ccctgaagtc caagctggtg tccgatttcc ggaaggattt ccagttttac aaagtgcgcg
     3901 agatcaacaa ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg ggaaccgccc
     3961 tgatcaaaaa gtaccctaag ctggaaagcg agttcgtgta cggcgactac aaggtgtacg
     4021 acgtgcggaa gatgatcgcc aagagcgagc aggaaatcgg caaggctacc gccaagtact
     4081 tcttctacag caacatcatg aactttttca agaccgagat taccctggcc aacggcgaga
     4141 tccggaagcg gcctctgatc gagacaaacg gcgaaaccgg ggagatcgtg tgggataagg
     4201 gccgggattt tgccaccgtg cggaaagtgc tgagcatgcc ccaagtgaat atcgtgaaaa
     4261 agaccgaggt gcagacaggc ggcttcagca aagagtctat cctgcccaag aggaacagcg
     4321 ataagctgat cgccagaaag aaggactggg accctaagaa gtacggcggc ttcgacagcc
     4381 ccaccgtggc ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac
     4441 tgaagagtgt gaaagagctg ctggggatca ccatcatgga aagaagcagc ttcgagaaga
     4501 atcccatcga ctttctggaa gccaagggct acaaagaagt gaaaaaggac ctgatcatca
     4561 agctgcctaa gtactccctg ttcgagctgg aaaacggccg gaagagaatg ctggcctctg
     4621 ccggcgaact gcagaaggga aacgaactgg ccctgccctc caaatatgtg aacttcctgt
     4681 acctggccag ccactatgag aagctgaagg gctcccccga ggataatgag cagaaacagc
     4741 tgtttgtgga acagcacaag cactacctgg acgagatcat cgagcagatc agcgagttct
     4801 ccaagagagt gatcctggcc gacgctaatc tggacaaagt gctgtccgcc tacaacaagc
     4861 accgggataa gcccatcaga gagcaggccg agaatatcat ccacctgttt accctgacca
     4921 atctgggagc ccctgccgcc ttcaagtact ttgacaccac catcgaccgg aagaggtaca
     4981 ccagcaccaa agaggtgctg gacgccaccc tgatccacca gagcatcacc ggcctgtacg
     5041 agacacggat cgacctgtct cagctgggag gtgactctgg aggatctagc ggaggatcct
     5101 ctggcagcga gacaccagga acaagcgagt cagcaacacc agagagctct ggtagcgaga
     5161 cacccggtac cagtgaaagc gccacgccag aaagcagtgg gagtgagact ccgggtacat
     5221 ctgaatcagc gacaccggaa tcaagtggcg gcagcagtgg cggcagcagc accctaaata
     5281 tagaagatga gtatcggcta catgagacct caaaagagcc agatgtttct ctagggtcca
     5341 catggctgtc tgattttcct caggcctggg cggaaaccgg gggcatggga ctggcagttc
     5401 gccaagctcc tctgatcata cctctgaaag caacctctac ccccgtgtcc ataaaacaat
     5461 accccatgtc acaagaagcc agactgggga tcaagcccca catacagaga ctgttggacc
     5521 agggaatact ggtaccctgc cagtccccct ggaacacgcc cctgctaccc gttaagaaac
     5581 cagggactaa tgattatagg cctgtccagg atctgagaga agtcaacaag cgggtggaag
     5641 acatccaccc caccgtgccc aacccttaca acctcttgag cgggccccca ccgtcccacc
     5701 agtggtacac tgtgcttgat ttaaaggatg cctttttctg cctgagactc caccccacca
     5761 gtcagcctct cttcgccttt gagtggagag atccagagat gggaatctca ggacaattga
     5821 cctggaccag actcccacag ggtttcaaaa acagtcccac cctgtttaat gaggcactgc
     5881 acagagacct agcagacttc cggatccagc acccagactt gatcctgcta cagtacgtgg
     5941 atgacttact gctggccgcc acttctgagc tagactgcca acaaggtact cgggccctgt
     6001 tacaaaccct agggaacctc gggtatcggg cctcggccaa gaaagcccaa atttgccaga
     6061 aacaggtcaa gtatctgggg tatcttctaa aagagggtca gagatggctg actgaggcca
     6121 gaaaagagac tgtgatgggg cagcctactc cgaagacccc tcgacaacta agggagttcc
     6181 tagggaaggc aggcttctgt cgcctcttca tccctgggtt tgcagaaatg gcagcccccc
     6241 tgtaccctct caccaaaccg gggactctgt ttaattgggg cccagaccaa caaaaggcct
     6301 atcaagaaat caagcaagct cttctaactg ccccagccct ggggttgcca gatttgacta
     6361 agccctttga actctttgtc gacgagaagc agggctacgc caaaggtgtc ctaacgcaaa
     6421 aactgggacc ttggcgtcgg ccggtggcct acctgtccaa aaagctagac ccagtagcag
     6481 ctgggtggcc cccttgccta cggatggtag cagccattgc cgtactgaca aaggatgcag
     6541 gcaagctaac catgggacag ccactagtca ttctggcccc ccatgcagta gaggcactag
     6601 tcaaacaacc ccccgaccgc tggctttcca acgcccggat gactcactat caggccttgc
     6661 ttttggacac ggaccgggtc cagttcggac cggtggtagc cctgaacccg gctacgctgc
     6721 tcccactgcc tgaggaaggg ctgcaacaca actgccttga tatcctggcc gaagcccacg
     6781 gaacccgacc cgacctaacg gaccagccgc tcccagacgc cgaccacacc tggtacacgg
     6841 atggaagcag tctcttacaa gagggacagc gtaaggcggg agctgcggtg accaccgaga
     6901 ccgaggtaat ctgggctaaa gccctgccag ccgggacatc cgctcagcgg gctgaactga
     6961 tagcactcac ccaggcccta aagatggcag aaggtaagaa gctaaatgtt tatactgata
     7021 gccgttatgc ttttgctact gcccatatcc atggagaaat atacagaagg cgtgggtggc
     7081 tcacatcaga aggcaaagag atcaaaaata aagacgagat cttggcccta ctaaaagccc
     7141 tctttctgcc caaaagactt agcataatcc attgtccagg acatcaaaag ggacacagcg
     7201 ccgaggctag aggcaaccgg atggctgacc aagcggcccg aaaggcagcc atcacagaga
     7261 ctccagacac ctctaccctc ctcatagaaa attcatcacc ctctggcggc tcaaaaagaa
     7321 ccgccgacgg cagcgaattc gagcccaaga agaagaggaa agtcggggga ggaggttctg
     7381 gaggcggagg ctccggaggc ggagggtcaa tgcccaagaa gaaacgcaag gtataccctt
     7441 atgacgtacc tgattacgcc ggtagctaca ggccagaatc cctcgacgta tgcatttacc
     7501 ttcgcaaatc caggaaggac gttgaagaag aacgccgcgc aatcgaagaa ggcagctcct
     7561 acaacgcact ggaacggcat cggaagcgat tgtttgccat tgccaaggca gaaaatcaca
     7621 acatcatcga tatttttgaa gaagttgcca gtggagagag catacaggaa agaccccaaa
     7681 tgcagcagct gctcaggaag ttggaaggca atgaaattga tggcgtgctg gtgattgatc
     7741 tcgatagact cgggcggggc gatatgctgg atgcgggaat gatcgatcgt gcattcagat
     7801 actcatctac caaaattatc accccaacag atgtctacga tcctgatgac gaaagttggg
     7861 agctggtgtt cgggattaag agtttaatca gccgacagga gctcaagtcc atcaccaaac
     7921 gactgcagaa tggccggatc gattcagtga aggaggggaa gcacattggc aagaagccac
     7981 cttatggcta cttgaaggat gagaatctga ggctgtatcc agatccagaa aaggcctgga
     8041 ttgtgaagaa gatttttgaa ctgatgtgtg acggaaaggg acggcagatg attgcggctg
     8101 agttggacag actgggtatt gacccccctg tgacgaaaag gggagcatgg gactctagta
     8161 ccatcaccag tattataaag aacgaagttt atacaggcgt cattgtctgg gggaaattta
     8221 agcataaaaa gaggaatggt aagtatacgc ggcataagaa cccacaggag aagtggatta
     8281 tgtacgagaa cgcccatgaa cccattatat ccaaagagct tttcgatgcg gcaaacgaag
     8341 cccatagctc cagacacaag cccgctgtca taacgagtaa aaagctgact aacccactgg
     8401 ctggcatctt gaagtgcaag ttgtgtggct acacaatgct catacagact cggaaggaca
     8461 ggcctcataa ctacttacga tgtaacaatc cagcctgtaa gggcaagcaa aaacagtcag
     8521 ttttcaattt agtggaggag aagttgctct attcactgca gcaaatcgtg gacgagtacc
     8581 aggcccagaa agttgaagag gtcgaaattg atgattctaa actcatctct tttaaggaaa
     8641 aggcaataat ctccaaagag aaggagctta aggagttaca agctcagaaa ggcaacctgc
     8701 atgacctgct cgaacaaggt atttacacgg tcgaaatctt cctggaacgg cagaagaatt
     8761 tggtggaaag aataaccagc atcgagaacg acatcgaggt gctgcagaag gagattgaaa
     8821 ctgagcagat caaagaacac aataagaccg agttcatccc cgccttaaaa acggtgatcg
     8881 aatcatatca caaaacaacc aatattgaac tcaaaaacca gctgctgaag accattctga
     8941 gcaccgtgac atactatagg catcccgact ggaaaaccaa tgaatttgaa atccaggtgt
     9001 acttcaaaat ctcctaggtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     9061 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     9121 ctgtcctttc ctaataaaat gagaaaattg catcgcattg tctgagtagg tgtcattcta
     9181 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     9241 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcga
     9301 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     9361 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     9421 agggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     9481 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     9541 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     9601 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     9661 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     9721 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     9781 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     9841 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     9901 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     9961 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    10021 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    10081 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    10141 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
    10201 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    10261 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    10321 gatctcaaga agatcctttg atcttttcta cggggtctga cactcagtgg aacgaaaact
    10381 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    10441 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    10501 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    10561 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    10621 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    10681 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    10741 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    10801 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    10861 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    10921 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    10981 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    11041 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    11101 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    11161 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    11221 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    11281 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    11341 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    11401 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    11461 cgcgcacatt tcc
//
