LOCUS       sequence-35177-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Kumar MS, Erkeland SJ, Pester RE, Chen CY, Ebert MS, Sharp PA, Jacks
            T
  TITLE     Suppression of non-small cell lung tumor development by the let-7 
            microRNA family.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Mar 11;105(10):3903-8. doi: 
            10.1073/pnas.0712321105
  PUBMED    18308936
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1073/pnas"; 
            journalName: "Proc Natl Acad Sci U S A"; date: "2008-03-11- 11"; 
            volume: "105"; issue: "10"; pages: "3903-8"
COMMENT     Sequence Label: MSCV puro let-7 sponge
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        244..743
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(279..302)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     676..695
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(764..783)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gccctcactc cttctctagg cgccggaatt agatctctcg aggcggccgc aactatacaa
       61 ggactacctc accggaacta tacaatgact acctcagcgg ccgcaactat acaaggacta
      121 cctcaccgga actatacaat gactacctca gcggccgcaa ctatacaagg actacctcac
      181 cggaactata cgatgactac ctcagcggcc gcctacacat tgatcctagg aacgaattct
      241 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc
      301 tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta
      361 ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag
      421 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag
      481 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc
      541 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa
      601 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt
      661 cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct
      721 cttcctcatc tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc
      781 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
      841 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagc
//
