LOCUS       sequence-351771-       11568 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of  V5-tagged mouse Sun1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11568)
  TITLE     Gerace lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV V5-Sun1
FEATURES             Location/Qualifiers
     source          1..11568
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2221..2432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2433..3371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3328..3348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3387..3396
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3396..3437
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6362..6911
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6506..6523)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6733..6752
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6914..7513
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6914..6933)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7410..7430
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7537..8125
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7590..7610)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8008..8019)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8332..8965
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9093..9109)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9093..9109)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9106..9128)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9117..9133
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9141..9147,9148..9165,9166..9171))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9186..9207
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9324..9341)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9495..10083)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9575..9594)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10254..11045,11046..11114))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10877..10896
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11115..11219)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11267..11401
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11304..11323)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11358..11377
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccgccg ccatgggtaa gcctatccct aaccctctcc
     3421 tcggtctcga ttctacgggg ggcggagggg gtgggcccat ggacttttct cggctgcaca
     3481 cgtacacccc accccagtgt gtgccggaga acactggcta cacttacgca ctcagttcta
     3541 gttactcgtc ggatgctctg gattttgaaa ctgagcacaa gttggaacct gtatttgact
     3601 ctccaaggat gtcccgccgc agcttgcgtc tggtcacaac agcttcgtac agcagtgggg
     3661 acagccaggc tattgattcg cacattagca ccagcagggc cacccccgcc aaggggagag
     3721 aaaccaggac agtcaaacag agaagaagtg caagcaagcc agcttttagt atcaaccacc
     3781 tgtcagggaa gggcttgtcc tcgagcacaa gccatgacag ctcttgcagc ctgcggagtg
     3841 ccacggtgct gcggcaccct gtgctagatg agtccctgat tcgtgagcag accaaagtgg
     3901 accacttctg gggtctcgat gatgatggtg accttaaagg tggaaataaa gctgccactc
     3961 agggaaatgg tgaactggca gcagaggtgg cgagcagcaa tggatacact tgccgtgact
     4021 gcaggatgct ctcagcgcgc actgacgcac tcacagccca ctctgccatc cacgggacca
     4081 cctccagggt gtactccaga gacaggactc tcaaaccacg cggagtgtcc ttttacctgg
     4141 ataggactct gtggctggcc aagtccacct cctcatcctt tgcatcattt atagttcaac
     4201 ttttccaagt ggttttaatg aagctcaatt ttgaaactta caaattgaaa ggctatgaat
     4261 ccagagctta tgaatcacag agctatgaga caaagagcca tgagtcagaa gcccatctcg
     4321 gtcactgtgg gaggatgact gccggagaac tttccagagt ggacggggag tccctgtgcg
     4381 atgactgtaa ggggaagaag caccttgaga tacacacagc cacccactcg caactgcccc
     4441 agccacacag ggtggccggg gccatggggc gcctctgcat ctatacaggt gacctcttgg
     4501 ttcaagcact gcgaaggact agagctgccg ggtggtctgt ggccgaggcc gtgtggtcgg
     4561 tgctctggct ggctgtctct gctccaggga aggcagcctc gggaaccttc tggtggctag
     4621 ggagcggctg gtaccaattt gttactttga tttcttggct gaatgtcttt cttcttacca
     4681 ggtgccttcg aaatatttgc aaggtttttg tcttgctcct cccactccta cttttactag
     4741 gtgctggtgt ctccctgtgg ggccagggaa acttcttctc actcctacca gtgctgaact
     4801 ggacggccat gcagccaaca cagagggtgg acgattccaa gggcatgcat agacctggcc
     4861 ctcttccccc gagcccacct ccaaaggttg atcacaaggc ttcccagtgg cctcaggaga
     4921 gtgacatggg gcagaaggta gcttctttga gtgcgcagtg ccacaaccat gatgagagac
     4981 ttgcagagct gacagtcctg cttcagaaac tacagatacg ggtagaccaa gtggatgacg
     5041 gcagggaagg gctgtcactg tgggtcaaga atgtggttgg acagcacctg caggagatgg
     5101 gcaccataga accacctgat gctaagactg acttcatgac tttccaccat gaccatgaag
     5161 tgcgtctctc caacttggaa gatgttctta gaaaactgac agaaaaatct gaggctatcc
     5221 agaaggagct ggaagaaacc aagctgaaag caggcagcag ggatgaagag cagcccctcc
     5281 ttgaccgtgt gcagcaccta gaactggaac tgaacctgtt gaagtcacag ctgtcagact
     5341 ggcagcatct gaagaccagc tgtgagcagg ctggggcccg catccaggag actgtgcagc
     5401 tcatgttctc tgaggatcag cagggcggtt ccctcgagtg gctattagag aagctttctt
     5461 ctcggttcgt gagcaaggat gagctgcagg tgctcttaca tgaccttgag ctgaaactgc
     5521 tgcagaatat cacacaccac atcaccgtga caggacaggc cccgacatcc gaggctattg
     5581 tgtctgccgt gaatcaggca gggatttcag gaatcacaga agcgcaagca catatcattg
     5641 tgaacaatgc tctgaagctg tactcccaag acaagacggg gatggtggac tttgctctgg
     5701 agtctggagg tggcagcatc ctaagcactc ggtgctctga gacctatgag accaagacgg
     5761 cactgctgag cctgtttggg gtcccactgt ggtacttctc acagtcacct cgagtggtga
     5821 tccagcccga catctaccca gggaattgct gggcgttcaa aggttcccag gggtacctgg
     5881 tggtgcggtt gtccatgaag atctacccaa ccacattcac catggaacac attccaaaga
     5941 cactatcacc cactggtaac atctccagtg cccccaaaga ctttgcagtc tatggactgg
     6001 aaacggagta tcaagaagag gggcagcctc tgggacggtt cacctatgac caggaaggag
     6061 actcactcca gatgttccac acactggaaa gacctgacca agccttccag atagtagagc
     6121 tccgggtcct gtccaactgg ggccaccctg agtacacttg cctctaccgg ttccgagtcc
     6181 acggagagcc catccagtag agcactccag ctaatgggcc gcgacgatgt ttaaacttcg
     6241 aaacgcgtga attcctcgag ggcggccgct ctagagtcga cgggccgcgg taacaattgt
     6301 taactaactt aagctagcaa cggtttccct ctagcgggat caattccgcc ccccccccct
     6361 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     6421 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     6481 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     6541 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     6601 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     6661 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     6721 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     6781 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
     6841 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     6901 acacgataat accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     6961 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     7021 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     7081 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     7141 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     7201 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     7261 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     7321 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     7381 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     7441 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     7501 gcccggtgcc tgacccgggc ggggcgcgtc tggaacaatc aacctctgga ttacaaaatt
     7561 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     7621 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     7681 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     7741 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     7801 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     7861 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     7921 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     7981 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     8041 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     8101 atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac ctagaaaaac
     8161 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
     8221 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga
     8281 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc
     8341 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     8401 acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg
     8461 gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag
     8521 agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     8581 tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc
     8641 cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact
     8701 ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat
     8761 aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
     8821 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     8881 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     8941 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     9001 taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta gcgtttaccg
     9061 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     9121 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     9181 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     9241 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     9301 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     9361 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     9421 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     9481 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     9541 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     9601 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     9661 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     9721 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     9781 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     9841 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     9901 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     9961 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    10021 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
    10081 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    10141 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
    10201 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
    10261 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
    10321 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
    10381 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
    10441 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
    10501 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    10561 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    10621 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    10681 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    10741 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    10801 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    10861 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    10921 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    10981 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    11041 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    11101 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    11161 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    11221 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcaact tgtttattgc
    11281 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    11341 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
    11401 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac
    11461 cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc
    11521 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagt
//
