LOCUS       sequence-351772-       11011 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of  V5-tagged mouse Sun2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11011)
  TITLE     Gerace lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV V5-Sun2
FEATURES             Location/Qualifiers
     source          1..11011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2221..2432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2433..3371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3328..3348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3387..3396
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3396..3437
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5805..6354
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5949..5966)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6176..6195
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6357..6956
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6357..6376)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6853..6873
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6980..7568
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7033..7053)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7451..7462)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7775..8408
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8536..8552)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8536..8552)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8549..8571)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8560..8576
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8584..8590,8591..8608,8609..8614))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8629..8650
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8767..8784)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8938..9526)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9018..9037)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9697..10488,10489..10557))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10320..10339
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10558..10662)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10710..10844
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10747..10766)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10801..10820
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccgccg ccatgggtaa gcctatccct aaccctctcc
     3421 tcggtctcga ttctacgggg ggcggagggg gtgggcccat gtcgagacga agccagcgcc
     3481 tcactcgcta ctctcaggat gataacgatg gcggcagcag cagcagtggt gcgagctccg
     3541 tggcaggaag ccagggcacc gtgtttaaag acagtcctct caggactttg aagaggaaat
     3601 ccagcaacat gaagcacctg tccccagctc cacagctggg cccctcctct gactcccaca
     3661 cctcctacta cagcgagtct gtggttcgag agtcctacat cggcagcccc cgggctgtgt
     3721 ccctcgccag gagtgccctc ctggatgacc acctacacag tgagccctac tggagcgggg
     3781 accttcgggg gaggaggagg agaggaacag gtggttctga gagcagcaag gccaatgggc
     3841 tcaccgcgga gagcaaggcc tcagaagact ttttcggatc ttcctcaggc tattcttcag
     3901 aggatgacct tgcaggctac acggactcag accagcacag ctcggggtcc aggttaagga
     3961 gtgcagcatc tcgggccggc tcctttgtct ggactctggt cacttttcca ggccgcctct
     4021 ttggtcttct ctactggtgg attggcacca cctggtaccg cctgacaact gctgcctccc
     4081 tcctggatgt cttcgtccta accaggtcca ggcacttctc gctgaacctg aagagttttc
     4141 tgtggttcct tctgctcttg ctactcctga ctggtctgac ctacggtgct tggcattttt
     4201 accccttagg gctgcagaca ttgcaacccg ctgtggtctc ctggtgggca gcaaaagaga
     4261 gcaggaagca gccagaggtg tgggaatcca gagacgcctc ccagcacttc caggctgagc
     4321 agcgcgttct ctcccgggtt cactctctgg agcggcgtct ggaagccctt gctgcagact
     4381 tttcctccaa ctggcagaag gaggccatac ggctggaacg cctggagctg cggcaggggg
     4441 ctgctggcca tggaggaggc agtagcctga gccatgaaga tgccctgtct ctcctagaag
     4501 ggttggtgag ccgccgcgag gctaccctga aggaggactt gcgcagggac acagtggctc
     4561 atatccagga agaattggct accctgaggg cagagcatca ccaagactcg gaagatctct
     4621 tcaagaagat cgtccaggcc tctcaggagt ccgaagcccg agtccagcag ctgaagacag
     4681 aatggaaaag catgacccag gaggccttcc aggagagctc tgtgaaggag ctgggacggc
     4741 tggaagccca gctggccagc ctgcggcagg agctggctgc cctgactctg aagcagaact
     4801 cggtggcaga tgaagtgggc ctgctgccac agaagatcca ggctgccagg gctgatgtgg
     4861 aatcccagtt ccctgactgg atcaggcagt tccttcttgg agacaggggt gcgcgcagcg
     4921 ggctcctgca gagagatgag atgcacgctc agctgcagga gctggagaac aagatcctta
     4981 ccaagatggc tgagatgcag ggcaagtcag ccagggaggc cgcagcgtcc ctgggacaga
     5041 tactgcagaa agaaggcata gttggggtga cagaggagca ggtgcaccgg atcgtcaagc
     5101 aggccctgca gcgctacagt gaggacagga ttggaatggt ggattacgcc ctggaatcag
     5161 gaggagccag tgttatcagc acccgctgct ctgagactta tgagaccaag acggcacttc
     5221 tcagcctctt tggcatcccc ctgtggtacc actcccagtc acctcgggtc attctgcagc
     5281 cagatgtgca cccaggcaac tgctgggcct tccaggggcc ccagggcttt gcagtggtcc
     5341 gcctctctgc tcgaatccga cctacagccg ttaccttaga gcacgtgccc aaggccttgt
     5401 cacccaacag cactatctcc agtgctccca aggacttcgc catctttggc ttcgatgaag
     5461 acctgcagca ggaagggaca cttcttggca cgtttgccta cgaccaggat ggggagccca
     5521 tccagacctt ctatttccag gcctctaaga tggccacata ccaagttgtg gagcttcgga
     5581 tcctgaccaa ctggggccac cctgagtaca catgtatcta ccgcttccgg gtgcacggag
     5641 agcctgccca ctagtaatgg gccgcgacga tgtttaaact tcgaaacgcg tgaattcctc
     5701 gagggcggcc gctctagagt cgacgggccg cggtaacaat tgttaactaa cttaagctag
     5761 caacggtttc cctctagcgg gatcaattcc gccccccccc cctaacgtta ctggccgaag
     5821 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     5881 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     5941 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     6001 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     6061 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     6121 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     6181 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     6241 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     6301 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat aataccatga
     6361 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     6421 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     6481 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     6541 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     6601 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     6661 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     6721 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     6781 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     6841 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     6901 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacccg
     6961 ggcggggcgc gtctggaaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     7021 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     7081 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     7141 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     7201 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     7261 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     7321 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     7381 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     7441 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     7501 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     7561 ctccccgcct ggaattaatt ctgcagtcga gacctagaaa aacatggagc aatcacaagt
     7621 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag
     7681 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta
     7741 gatcttagcc actttttaaa agaaaagagg ggactggaag ggctaattca ctcccaacga
     7801 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag
     7861 aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta
     7921 gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta
     7981 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt
     8041 gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac
     8101 tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc
     8161 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc
     8221 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     8281 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     8341 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     8401 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     8461 aatatcagag agtgagaggc cttgacattg ctagcgttta ccgtcgacct ctagctagag
     8521 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     8581 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     8641 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     8701 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     8761 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8821 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8881 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8941 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     9001 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     9061 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     9121 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     9181 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     9241 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     9301 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     9361 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     9421 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     9481 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9541 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     9601 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     9661 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9721 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9781 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9841 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9901 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9961 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    10021 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    10081 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    10141 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    10201 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    10261 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    10321 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    10381 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    10441 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    10501 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    10561 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    10621 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    10681 gccacctgac gtcgacggat cgggagatca acttgtttat tgcagcttat aatggttaca
    10741 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
    10801 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag
    10861 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt
    10921 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta
    10981 tttgttaaat atgtactaca aacttagtag t
//
