LOCUS       sequence-351773-       10838 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of mouse Ndc1-V5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10838)
  TITLE     Gerace lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV Ndc1-V5
FEATURES             Location/Qualifiers
     source          1..10838
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2221..2432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2433..3371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3328..3348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3402..3411
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5454..5495
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5632..6181
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5776..5793)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6003..6022
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6184..6783
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6184..6203)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6680..6700
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6807..7395
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6860..6880)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7278..7289)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7602..8235
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8363..8379)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8363..8379)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8376..8398)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8387..8403
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8411..8417,8418..8435,8436..8441))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8456..8477
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8594..8611)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8765..9353)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8845..8864)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9524..10315,10316..10384))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10147..10166
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10385..10489)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10537..10671
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10574..10593)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10628..10647
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccgcca tcggcccgcc cgccgccatg gccacggctg
     3421 cgagcgggcc ctgcgccggc gggtcgccgc gggacatcct ttggcgcgtt ttgggttgga
     3481 ggatagttac cagcatcgtt tggtcagtag tgcttctacc cgtctgcatc acagccttta
     3541 tagtcttaag cagcatcaat ttattccacc ctatacagtg gctgtcagat tcttgcaatg
     3601 acttctatag ttcccaagtc atctttcacc tcctgctgct ggccgtggtc attataataa
     3661 taagtatttt caatgtggaa ttctatacag ttgtgccctc catatccggc tccagactgg
     3721 ccctgatagc gcggatcctg catccccagc agctcacgca ctccttcatc cacgccgcga
     3781 tgggaatggc tgtggcttgg tgtgcggcca tcatgaccaa gggccagtac agctctctcg
     3841 tggttccctg cactggaact gagagcttag atagccctgc tgctcagacc tgcttgaatg
     3901 aatatcatct gtttttccta ctgtccggag cattcatggg ctatagctat agtcttctgt
     3961 atttcattaa caacatgaat tatctcccgt ttcctattat acagcagtac aaatttttgc
     4021 gtttccggag atctctcctc ttactagtta aacatagctg tgtggaatcc ctgttcatgg
     4081 tcagaaattt ctgcattgtg tattattttt ttggccatat tcccaaagca tggattagca
     4141 ctgctctgga ccttcacaca gatgagcagg ctcacaggcc tcttgacacg ataggcggtc
     4201 tcctgaacgt ctcattactc taccacgtct ggctgtgtgg cgtcttcctc ttggtgactt
     4261 ggtatagctc atggatactc ttcaaaatct atgccaccga ggcgcatgtg ttccctgttc
     4321 agccgccttt tgcagaagcc tcagatgaat gccttcctaa agtcttaaac agcaaccccc
     4381 cacgcatcgt aaagtatttg gccttacagg acctgatgct gctttctcaa tattctcctt
     4441 ctcggaggca agaagttttc agtctcagcc aaccaggtgg acatccccac aattggactg
     4501 ctatttcgag ggagtgtttg aatcttttaa atgatatgac tcagaaactg gttctctacc
     4561 aagaagctgc tgctacaaat gggaggatgt attcatcata ctcagtggaa cccaagaaac
     4621 tgagctctgc agaagaaact gcttttcaga ctccaaaacc tagtcagacg ccttccgtgc
     4681 cgccgttagt taaaacttca ctgttttctc caaaattatc tacacccaat gtttcaagtc
     4741 cttttgggac cccgtttggc tccagcgtag tgaaccgaat ggctggaatt cttgatgtaa
     4801 accccttctc tgggtctccc caaagtcctc agctcataag aagaggaccg aggctgtgga
     4861 ctcacacttc tgatcaacaa gtgtctgcaa tttctaaccc atctccttgc gcctctgtta
     4921 ctgctgaggg taagacagtg agacagccca gtgtgatcta ctcgtggatt cagaataaac
     4981 gagaacagat taagaatttc ttgtcaaaac gggtgctgat aatgtacttt ttcagtaagc
     5041 acccagaggc ctccattcag gctgtttttt cagatgccca aatgcatatt tgggctttag
     5101 aaggtctgtc tcacttagta gcagcgtcat ttacagaaga tagatttgga gttgtccaga
     5161 ccacactccc agctatcctt catacgctgt tgactctgca ggaggctgtc gacaagtact
     5221 tcaagctgcc tcatgcttct agtaaaccac cccgagcttc aggaagcctt gtggacacgt
     5281 cctacaaaac attgagattt gctttcaggg cctccctgaa aaccgctatc taccgtataa
     5341 ccaccacgtt tggtgaacat ctgaacgctg tgcaggcatc tgcagaacat cagaaaagac
     5401 ttcagcaatt cttggagttc aaagaaggtg ggccgggcgg gggtggcggg ggaggtaagc
     5461 ctatccctaa ccctctcctc ggtctcgatt ctacgtgagt ttaaacttcg aaacgcgtga
     5521 attcctcgag ggcggccgct ctagagtcga cgggccgcgg taacaattgt taactaactt
     5581 aagctagcaa cggtttccct ctagcgggat caattccgcc ccccccccct aacgttactg
     5641 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
     5701 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
     5761 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag
     5821 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc
     5881 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac
     5941 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
     6001 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt
     6061 gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa
     6121 aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgataat
     6181 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     6241 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     6301 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     6361 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     6421 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     6481 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     6541 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     6601 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     6661 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     6721 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     6781 tgacccgggc ggggcgcgtc tggaacaatc aacctctgga ttacaaaatt tgtgaaagat
     6841 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     6901 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     6961 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     7021 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     7081 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     7141 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     7201 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     7261 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     7321 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     7381 gggccgcctc cccgcctgga attaattctg cagtcgagac ctagaaaaac atggagcaat
     7441 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga
     7501 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc
     7561 agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc taattcactc
     7621 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     7681 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
     7741 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
     7801 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
     7861 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
     7921 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
     7981 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
     8041 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     8101 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     8161 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     8221 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     8281 agaaatgaat atcagagagt gagaggcctt gacattgcta gcgtttaccg tcgacctcta
     8341 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     8401 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     8461 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     8521 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     8581 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     8641 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     8701 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     8761 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     8821 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     8881 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     8941 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     9001 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     9061 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     9121 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     9181 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     9241 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     9301 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     9361 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     9421 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     9481 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     9541 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     9601 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     9661 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     9721 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     9781 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     9841 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     9901 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     9961 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
    10021 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
    10081 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
    10141 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    10201 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    10261 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    10321 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    10381 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    10441 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    10501 gaaaagtgcc acctgacgtc gacggatcgg gagatcaact tgtttattgc agcttataat
    10561 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
    10621 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata
    10681 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cactgccaat
    10741 tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga
    10801 aattgtattt gttaaatatg tactacaaac ttagtagt
//
