LOCUS       sequence-351783-        4458 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC6A8_STOP
FEATURES             Location/Qualifiers
     source          1..4458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2015..2114)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2131..2150)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2132..2150)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2155..2171)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2155..2171)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2273..2292)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2284..3093
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2351..2370)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3240..3828
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3729..3748
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3982..3999
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4158..4185)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4277..4363)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4412..4434
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4426..4443
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4427..4443
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggc caagaagtct
      121 gccgagaatg gcatctactc cgtgtctggc gacgagaaga agggccccct gatcgcccca
      181 ggccccgacg gcgccccagc caagggcgat ggccccgtgg gcctgggcac acctggaggc
      241 cggctggcag tgccacctag ggagacatgg accagacaga tggacttcat catgagctgc
      301 gtgggattcg cagtgggact gggaaacgtg tggagatttc cttacctgtg ctataagaac
      361 ggcggcggcg tgttcctgat cccatacgtg ctgatcgccc tggtgggagg aatccccatc
      421 ttctttctgg agatctctct gggccagttc atgaaggccg gcagcatcaa cgtgtggaac
      481 atctgtccac tgtttaaggg cctgggctac gcctccatgg tcatcgtgtt ctactgcaat
      541 acatactata tcatggtgct ggcctggggc ttttactatc tggtgaagtc tttcaccaca
      601 accctgcctt gggcaacctg cggacacaca tggaacaccc cagactgcgt ggagatcttt
      661 aggcacgagg attgcgccaa tgcctccctg gccaacctga cctgtgacca gctggccgat
      721 cggagatctc cagtgatcga gttctgggag aataaggtgc tgaggctgag cggaggactg
      781 gaggtgccag gcgccctgaa ctgggaggtg acactgtgcc tgctggcctg ttgggtgctg
      841 gtgtactttt gcgtgtggaa gggcgtgaag tccaccggca agatcgtgta ctttacagcc
      901 accttcccct atgtggtgct ggtggtgctg ctggtgcggg gcgtgctgct gcctggcgcc
      961 ctggacggca tcatctacta tctgaagcca gattggtcca agctgggatc tccacaagtg
     1021 tggatcgacg caggcacaca gatcttcttt tcttatgcca tcggactggg cgccctgacc
     1081 gccctgggaa gctacaatag gttcaacaat aactgctata aggatgccat catcctggcc
     1141 ctgatcaact ctggcacaag cttctttgcc ggctttgtgg tgttcagcat cctgggcttt
     1201 atggccgccg agcagggcgt gcacatcagc aaggtggcag agtccggacc tggactggcc
     1261 ttcatcgcat acccaagggc agtgacactg atgccagtgg cacctctgtg ggccgccctg
     1321 ttctttttca tgctgctgct gctgggcctg gacagccagt ttgtgggcgt ggagggcttc
     1381 atcaccggac tgctggatct gctgccagca tcctactact tccggttcca gcgcgagatc
     1441 tctgtggccc tgtgctgtgc cctgtgcttt gtgatcgacc tgtccatggt gaccgatggc
     1501 ggcatgtacg tgtttcagct gttcgactac tatagcgcct ccggaacaac cctgctgtgg
     1561 caggcctttt gggagtgcgt ggtggtggca tgggtgtacg gagcagatag gttcatggac
     1621 gatatcgcct gcatgatcgg ctatcgccct tgcccatgga tgaagtggtg ttggtccttt
     1681 ttcacacctc tggtgtgcat gggcatcttt atcttcaacg tggtgtacta tgagcccctg
     1741 gtgtacaata acacctacgt gtatccttgg tggggagagg caatgggatg ggccttcgcc
     1801 ctgagctcca tgctgtgcgt gcctctgcac ctgctgggat gtctgctgag ggcaaaggga
     1861 acaatggccg agagatggca gcacctgacc cagccaatct ggggcctgca ccacctggag
     1921 tatagggcac aggacgcaga tgtgcgcgga ctgacaaccc tgacccccgt gagcgagtct
     1981 agcaaggtgg tggtggtgga gtccgtgatg taagacccag ctttcttgta caaagttggc
     2041 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
     2101 aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata
     2161 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     2221 aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
     2281 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct
     2341 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
     2401 cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     2461 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     2521 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     2581 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     2641 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     2701 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     2761 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     2821 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     2881 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
     2941 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     3001 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     3061 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac
     3121 tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac
     3181 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3241 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3301 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3361 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3421 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3481 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3541 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3601 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3661 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3721 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3781 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3841 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3901 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3961 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     4021 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     4081 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     4141 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     4201 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4261 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4321 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4381 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4441 agtcttaagc tcgggccc
//
