LOCUS       sequence-351787-        3870 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing GATA4 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-GATA4
FEATURES             Location/Qualifiers
     source          1..3870
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1427..1526)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1543..1562)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1544..1562)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1685..1704)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1696..2505
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1763..1782)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2652..3240
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3141..3160
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3394..3411
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3570..3597)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3689..3775)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3824..3846
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3838..3855
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3839..3855
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tgtaccagag cctggctatg
      121 gctgctaatc atggacctcc ccctggagcc tatgaagccg gaggacctgg cgcttttatg
      181 catggagctg gcgccgcttc ttctcccgtg tatgtgccta cacctagagt gcccagcagc
      241 gtgctgggcc tttcttatct tcagggagga ggagcaggat ctgcttctgg cggagcttca
      301 ggcggatctt ctggaggcgc tgcttcaggt gctggacctg gaactcaaca gggatctcct
      361 ggatggtcac aggcaggagc tgatggagcc gcttataccc ctcctcctgt gagccccagg
      421 tttagctttc ctggcacaac aggctcttta gctgccgctg ctgctgcagc cgcagctaga
      481 gaagcagctg catattctag tggcggagga gctgctggag ccggcttagc tggaagagag
      541 cagtacggaa gagccggatt tgccggaagc tatagcagcc cttaccctgc ctatatggcc
      601 gatgttggcg catcttgggc agccgccgca gcagcttctg caggaccttt tgactcacct
      661 gtgcttcact ctctgcctgg cagagctaat cctgccgcca gacatcccaa cctggacatg
      721 ttcgacgact tcagcgaggg cagagaatgc gtgaactgcg gagccatgag cacccccctt
      781 tggagaagag acggcaccgg ccactacctt tgcaatgcct gtggcctgta ccacaagatg
      841 aacggcatca acagacccct gatcaagccc cagagaagac tgagcgctag cagaagagtg
      901 ggcctgtcct gcgccaattg ccagaccaca accaccacac tgtggaggag aaatgccgag
      961 ggcgagcctg tgtgtaacgc ctgtggactg tacatgaagc tgcacggcgt gcccagacct
     1021 ctggccatga gaaaggaggg catccagacc agaaagagaa agcccaagaa cctgaacaag
     1081 agcaagaccc ccgctgctcc ttctggaagc gagagcctgc ctccagcctc tggagccagc
     1141 agcaatagct ctaacgccac cacatcttct tctgaggaga tgaggcccat caaaaccgag
     1201 ccaggcctga gcagccacta cggccacagc tctagcgtga gccagacttt tagcgtgtct
     1261 gccatgtcag gccacggacc tagcattcac cctgtgctga gcgccctgaa gttgagccca
     1321 cagggctatg cttctcctgt gtctcagagc cctcagacct ccagcaagca ggactcctgg
     1381 aattctctgg tgctggccga cagccacggc gatatcatca ccgcctgccc aactttcttg
     1441 tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact
     1501 atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat
     1561 tacatggtca tagctgtttc ttggcagctc tggcccgtgt ctcaaaatct ctgatgttac
     1621 attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt
     1681 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc
     1741 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
     1801 gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
     1861 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa
     1921 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
     1981 accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt
     2041 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt
     2101 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat
     2161 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
     2221 gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt
     2281 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
     2341 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     2401 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     2461 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat
     2521 tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca
     2581 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc
     2641 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2701 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2761 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     2821 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2881 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2941 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3001 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3061 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3121 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3181 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3241 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3301 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3361 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     3421 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     3481 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc
     3541 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
     3601 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
     3661 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
     3721 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc
     3781 tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt
     3841 aaaacgacgg ccagtcttaa gctcgggccc
//
