LOCUS       sequence-35179-e         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Vierbuchen T, Ostermeier A, Pang ZP, Kokubu Y, Sudhof TC, Wernig M
  TITLE     Direct conversion of fibroblasts to functional neurons by defined 
            factors.
  JOURNAL   Nature. 2010 Feb 25. 463(7284):1035-41.
  PUBMED    20107439
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Feb 25. 463(7284):1035-41."
COMMENT     Sequence Label: Tet-O-FUW-EGFP
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(99..123)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(120..158)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(191..209,210..232,233..251,252..274,275..293,294..316,
                     317..335,336..358,359..377,378..400,401..419,420..442,
                     443..461)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     misc_feature    518..635
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ccttgctcac catggtggcg gaattcgggg ccgcggaggc tggatcggtc ccggtgtctt
       61 ctatggaggt caaaacagcg tggatggcgt ctccaggcga tctgacggtt cactaaacga
      121 gctctgctta tataggcctc ccaccgtaca cgcctacctc gacccgggta ccgagctcga
      181 ctttcacttt tctctatcac tgatagggag tggtaaactc gactttcact tttctctatc
      241 actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa
      301 actcgacttt cacttttctc tatcactgat agggagtggt aaactcgact ttcacttttc
      361 tctatcactg atagggagtg gtaaactcga ctttcacttt tctctatcac tgatagggag
      421 tggtaaactc gactttcact tttctctatc actgataggg agtggtaaac tcgagatcta
      481 accaaactgg atctctgctg tccctgtaat aaacccgaaa attttgaatt tttgtaattt
      541 gtttttgtaa ttctttagtt tgtatgtctg ttgctattat gtctactatt ctttcccctg
      601 cactgtaccc cccaatcccc ccttttcttt taaaattgtg gatgaatact gccatttgtc
      661 tgcagaattg gcgcacgcag tgccgatccg ttcactaatc gaatggatct gtctctgtct
      721 ctctctccac cttcttcttc tattccttcg ggcctgtcgg gtcccctcgg g
//
