LOCUS       sequence-35182-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Kim do J, Kim HS, Lee SJ, Suh SW
  TITLE     Crystal structure of Thermotoga maritima SPOUT superfamily RNA 
            methyltransferase Tm1570 in complex with S-adenosyl-L-methionine.
  JOURNAL   Proteins. 2009 Jan . 74(1):245-9.
  PUBMED    18831037
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proteins. 
            2009 Jan . 74(1):245-9."
COMMENT     Sequence Label: pET28b-TM1570
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             74..91
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             689..706
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(759..777)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      773..820
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gaatattttg tttactttaa gaaggagata taccatgggc agcagccatc atcatcatca
       61 tcacagcagc ggcctggtgc cgcgcggcag ccatatgtta gagaaggtgt acgtggccct
      121 gattcattac cctataaaag gaaaagacgg cagcatcata tcaacggccg tgacaaacct
      181 cgatgtccac gatatagcaa ggactgcgag aacgtacaac ctcaagggat actacattgt
      241 gactaacctc agagcgcagc aggatatggt aagcaagatg ttaaaattct ggagagaagg
      301 ttttggaagc cggtacaatc cttccagagc ggaatctctc aagctcgtga agctcaaatc
      361 ctatctggag gatgtgctgg aagacataga aagtgtcgaa ggagaaagac ctttgatctt
      421 cttcacctct gcgaaaaaac gcgaaaatga catttctttc gaagagggaa gaagaataat
      481 catagaaacc gaaaaacctg ttctcatact tctcggtaca ggctggggtc ttcccgatga
      541 aattctcgag atatccgatt acgtactgga gcccataagg gcgcagtctg atttcaacca
      601 tctttccgtg agagcggctg ctgccatcat catcgatcgg ttgataggag aaaattacgc
      661 gaggagggat taagcggccg cactcgagca ccaccaccac caccactgag atccggctgc
      721 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
      781 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
      841 cggattggcg aatgggacgc gccctgtagc ggcgcattta gcgcg
//
