LOCUS       sequence-351833-        7271 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FBP17 c-terminal labeled with mScarelt in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7271)
  AUTHORS   Taylor KL, Taylor RJ, Richters KE, Huynh B, Carrington J, McDermott 
            ME, Wilson RL, Dent EW
  TITLE     Opposing functions of F-BAR proteins in neuronal membrane 
            protrusion, tubule formation, and neurite outgrowth.
  JOURNAL   Life Sci Alliance. 2019 Jun 3;2(3). pii: 2/3/e201800288. doi: 
            10.26508/lsa.201800288. Print 2019 Jun.
  PUBMED    31160379
REFERENCE   2  (bases 1 to 7271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2019-06-3"; volume: "2(3). pii: 2/3/e201800288. 
            doi"; pages: " 10.26508/lsa.201800288. Print 2019 Jun"
COMMENT     Sequence Label: pCAX-mScarlet-FBP17
FEATURES             Location/Qualifiers
     source          1..7271
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3591..4286
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(3724..3742)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3992..4011
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(4293..4312)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4358..4413
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4412..4431)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4774..4790)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4774..4790)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4787..4809)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4798..4814
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4822..4828,4829..4846,4847..4852))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4867..4888
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4947..5142
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4993..5128
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5148..5282
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5185..5204)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5239..5258
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5350..5367)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5521..6109)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5601..5620)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6280..7071,7072..7140))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6903..6922
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7141..7245)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggsgcgc gccrggsggg gsggggsggg gsgrggggsg
      541 gggsggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcaggtacca
     1741 tgagctgggg caccgagctc tgggatcagt ttgacaactt agaaaaacac acacagtggg
     1801 gaattgatat tcttgagaaa tatatcaagt ttgtgaaaga aaggacagag attgaactca
     1861 gctatgcaaa gcaactcagg aatctttcaa agaagtacca acctaaaaag aactcgaagg
     1921 aggaagaaga atacaagtat acgtcatgta aagctttcat ttccaacctg aacgaaatga
     1981 atgattacgc agggcagcat gaagttatct ccgagaacat ggcatcacag atcattgtgg
     2041 acttggcacg ctatgttcag gaactgaaac aggagaggaa atcaaacttt cacgatggcc
     2101 gtaaagcaca gcagcacatc gagacttgct ggaagcagct tgaatctagt aaaaggcgat
     2161 ttgaacgcga ttgcaaagag gcggacaggg cgcagcagta ctttgagaaa atggacgctg
     2221 acatcaatgt cacaaaagcg gatgttgaaa aggcccgaca acaagctcaa atacgtcacc
     2281 aaatggcaga ggacagcaaa gcagattact catccattct ccagaaattc aaccatgagc
     2341 agcatgaata ttaccatact cacatcccca acatcttcca gaaaatacaa gagatggagg
     2401 aaaggaggat tgtgagaatg ggagagtcca tgaagacata tgcagaggtt gatcggcagg
     2461 tgatcccaat cattgggaag tgcctggatg gaatagtaaa agcagccgaa tcaattgatc
     2521 agaaaaatga ttcacagctg gtaatagaag cttataaatc agggtttgag cctcctggag
     2581 acattgaatt tgaggattac actcagccaa tgaagcgcac tgtgtcagat aacagccttt
     2641 caaattccag aggagaaggc aaaccagacc tcaaatttgg tggcaaatcc aaaggaaagt
     2701 tatggccgtt catcaaaaaa aataagctta tgtccctttt aacatccccc catcagcctc
     2761 cccctccccc tcctgcctct gcctcaccct ctgctgttcc caacggcccc cagtctccca
     2821 agcagcaaaa ggaacccctc tcccaccgct tcaacgagtt catgacctcc aaacccaaaa
     2881 tccactgctt caggagccta aagcgtgggg gtgcaacacc ggacgatttc agcaacctcc
     2941 cacctgaaca aagaaggaaa aagctgcagc agaaagtcga tgagttaaat aaagaaattc
     3001 agaaggagat ggatcaaaga gatgccataa caaaaatgaa agatgtctac ctaaagaatc
     3061 ctcagatggg agacccagcc agtttggatc acaaattagc agaagtcagc caaaatatag
     3121 agaaactgcg agtagagacc cagaaatttg aggcctggct ggctgaggtt gaaggccggc
     3181 tcccagcacg caacgagcag gcgcgccggc agagcggact gtacgacagc cagaacccac
     3241 ccacagtcaa caactgcgcc caggaccgtg agagcccaga tggcagttac acagaggagc
     3301 agagtcagga gagtgagatg aaggtgctgg ccacggattt tgacgacgag tttgatgatg
     3361 aggagcccct ccctgccata gggacgtgca aagctctcta cacatttgaa ggtcagaatg
     3421 aaggaacgat ttccgtagtt gaaggagaaa cattgtatgt catagaggaa gacaaaggcg
     3481 atggctggac ccgcattcgg agaaatgaag atgaagaggg ttatgtcccc acttcatatg
     3541 tcgaagtctg tttggacaaa aatgccaaag attcctgcgg ccgcctcgag atggtgagca
     3601 agggcgaggc agtgatcaag gagttcatgc ggttcaaggt gcacatggag ggctccatga
     3661 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     3721 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt ctcctgggac atcctgtccc
     3781 ctcagttcat gtacggctcc agggccttca tcaagcaccc cgccgacatc cccgactact
     3841 ataagcagtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     3901 gcgccgtgac cgtgacccag gacacctccc tggaggacgg caccctgatc tacaaggtga
     3961 agctccgcgg caccaacttc cctcctgacg gccccgtaat gcagaagaag acaatgggct
     4021 gggaagcgtc caccgagcgg ttgtaccccg aggacggcgt gctgaagggc gacattaaga
     4081 tggccctgcg cctgaaggac ggcggacgct acctggcgga cttcaagacc acctacaagg
     4141 ccaagaagcc cgtgcagatg cccggcgcct acaacgtcga ccgcaagttg gacatcacct
     4201 cccacaacga ggactacacc gtggtggaac agtacgaacg ctccgagggc cgccactcca
     4261 ccggcggcat ggacgagctg tacaagtaaa gatctttttc cctctgccaa aaattatggg
     4321 gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt
     4381 gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat
     4441 catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg
     4501 gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct
     4561 gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt
     4621 tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga
     4681 tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc
     4741 ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     4801 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     4861 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     4921 gggaaacctg tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc
     4981 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     5041 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     5101 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg
     5161 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     5221 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     5281 tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     5341 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     5401 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     5461 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     5521 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     5581 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     5641 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     5701 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     5761 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     5821 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     5881 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     5941 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     6001 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     6061 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     6121 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     6181 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     6241 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     6301 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     6361 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     6421 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     6481 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     6541 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     6601 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     6661 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     6721 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     6781 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     6841 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     6901 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     6961 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     7021 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     7081 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     7141 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     7201 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     7261 agtgccacct g
//
