LOCUS       sequence-35185-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Lee SJ, Kim HS, Kim do J, Yoon HJ, Kim KH, Yoon JY, Suh SW
  TITLE     Crystal structures of LacD from Staphylococcus aureus and LacD.1 
            from Streptococcus pyogenes: insights into substrate specificity and
            virulence gene regulation.
  JOURNAL   FEBS Lett. 2011 Jan 21. 585(2):307-12.
  PUBMED    21192932
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2011 Jan 21. 585(2):307-12."
COMMENT     Sequence Label: LacD_L275S_pET21a
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(32..49)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gctcttcggg cttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagttttc
       61 tttgtttcca agatgtcgca gtatctttta atactttatt taagtcatca atatttttga
      121 aaccagttgt acgtaaccat tcacgagcag catcttcacc ttgttcaatg tatacttgaa
      181 cagcaccaga ccatgtagca cggccacaag ataccccgtt gaatttagca ccagcttcgt
      241 gagcaaattt taaagtttct tggaataatt ccgcagaaac accagcactt aagtaaatgt
      301 atggtaaatg agttgctgca tcttgatctt taaagtgttg tgccgcttcc tcttttgtat
      361 aaaccacttc accttcagca aagccttcta catatttcat gtttactggt acttcaactt
      421 tcaatacatc aacgttaaag cgtggttctg agaataattt cattgcttcg ttaacctttc
      481 taggcttaac ttttgcgaat tcaacactac cgttatcagg aatgttgtca tcgtatgtta
      541 atacctctaa aaagaatgga atatcttctg caacacattc tgaaccgatt ctttcaatgt
      601 atgctttctt ttgaatgtta atttcttcag catcatcaac atcatagtaa agtaagaatt
      661 taacagcatt tgcgccttgt tcttttaaac gttttgcaga ccactctact aaacagtcag
      721 gtaaacgacc tttagcgttt acgtcatatc cagttttttc ataagcaagt aataatccac
      781 aatctttgtt acgtgcatct gaagctggta aaccatactc aggatctaat aaaatgaaga
      841 tgcatatggg gttaatcct
//
