LOCUS       sequence-35186-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Lee SJ, Kim HS, Kim do J, Yoon HJ, Kim KH, Yoon JY, Suh SW
  TITLE     Crystal structures of LacD from Staphylococcus aureus and LacD.1 
            from Streptococcus pyogenes: insights into substrate specificity and
            virulence gene regulation.
  JOURNAL   FEBS Lett. 2011 Jan 21. 585(2):307-12.
  PUBMED    21192932
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2011 Jan 21. 585(2):307-12."
COMMENT     Sequence Label: LacD_L165E/L275S_pET21a
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gctcttcggg cttgtagcag ccggatctca gtggtggtgg tggtggtgct cgagttttct
       61 ttgtttccaa gatgtcgcag tatcttttaa tactttattt aagtcatcaa tatttttgaa
      121 accagttgta cgtaaccatt cacgagcagc atcttcacct tgttcaatgt atacttgaac
      181 agcaccagac catgtagcac ggccacaaga taccccgttg aatttagcac cagcttcgtg
      241 agcaaatttt aaagtttctt ggaataattc cgcagaaaca ccagcactta agtaaatgta
      301 tggtaaatga gttgctgcat cttgatcttt aaagtgttgt gccgcttcct cttttgtata
      361 aaccacttca ccttcagcaa agccttctac atatttcatg tttactggta cttcaacttt
      421 caatacatca acgttaaagc gtggttctga gaataatttc attgcttcgt taacctttct
      481 aggcttaact tttgcgaatt caacactacc gttatcagga atgttgtcat cgtatgtttc
      541 tacttctaaa aagaatggaa tatcttctgc aacacattct gaaccgattc tttcaatgta
      601 tgctttcttt tgaatgttaa tttcttcagc atcatcaaca tcatagtaaa gtaagaattt
      661 aacagcattt gcgccttgtt cttttaaacg ttttgcagac cactctacta aacagtcagg
      721 taaacgacct ttagcgttta cgtcatatcc agttttttca taagcaagta ataatccaca
      781 atctttgtta cgtgcatctg aagctggtaa accatactca ggatctaata aaattgaaga
      841 tgcatattgt gttaattctt ccgcaactaa
//
