LOCUS       sequence-35187-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Lee SJ, Kim HS, Kim do J, Yoon HJ, Kim KH, Yoon JY, Suh SW
  TITLE     Crystal structures of LacD from Staphylococcus aureus and LacD.1 
            from Streptococcus pyogenes: insights into substrate specificity and
            virulence gene regulation.
  JOURNAL   FEBS Lett. 2011 Jan 21. 585(2):307-12.
  PUBMED    21192932
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2011 Jan 21. 585(2):307-12."
COMMENT     Sequence Label: LacD.1_WT_pET21a
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(783..815)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     protein_bind    865..881
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tagttcgaca atcttacctg
       61 tttcaaattg agatcgtaac atatgttacg atctcaattt gaagaagatt tagagaaatt
      121 acataaccag ttctacgcta tgggacaaga agtgctctca caaatcaatc gtacggtacg
      181 tgcttttgtc acgcatgacc gtgacctggc aaaagaggtc atcgaagatg atgcagaagt
      241 aaatgaatac gaagtgaaac tggaaaagaa atcatttgaa atgatcgcac tccaacaacc
      301 agtctctcaa gatttgcgta cagtcttgac tgtccttaag gctgtatcag atgtggagcg
      361 tatgggggat cacgctgtag ccattgccca ggcaaccatc cgtatgaagg gggaagagcg
      421 cattccagct gtagaggaag aaattaaaaa aatgggacgt gaagttaaaa gcgttgtgga
      481 agcagcactt gatctttatc ttaatggttc tgttgacgac gcataccggg tggcctccat
      541 ggatgagcaa attaaccact attttgaaac tatccgtgac cttgcgactg aagagattaa
      601 gaaaaatcca gaagccattg ttacgggtcg tgattatttc caagttattt cctacttgga
      661 gcgtatcggt gactatgcta aaaatatctg tgaatggggt tgtctacttt gaaaacaggt
      721 aagattgtcg aactactcga gtgcggccgc aagcttgtcg acggagctcg aattcggatc
      781 cgacccattt gctgtccacc agtcatgcta gccatatgta tatctccttc ttaaagttaa
      841 accaaaatta tttctagagg ggaattgtta tccgctcaca at
//
