LOCUS       sequence-351936-        9983 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of mouse Rbmxl1-V5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9983)
  TITLE     Gerace lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV Rbmxl1-V5
FEATURES             Location/Qualifiers
     source          1..9983
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2221..2432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2433..3371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3328..3348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3402..3411
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4599..4640
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4777..5326
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4921..4938)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5148..5167
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5329..5928
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5329..5348)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5825..5845
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5952..6540
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6005..6025)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6423..6434)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6747..7380
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7508..7524)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7508..7524)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7521..7543)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7532..7548
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7556..7562,7563..7580,7581..7586))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7601..7622
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7739..7756)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7910..8498)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7990..8009)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8669..9460,9461..9529))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9292..9311
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9530..9634)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9682..9816
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9719..9738)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9773..9792
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccgcca tcggcccgcc cgccgccatg gttgaagcag
     3421 atcgcccagg aaagctcttc attggtgggc ttaatacaga aacaaatgag aaagcccttg
     3481 aagcagtatt tggcaagtat ggacgaatag tggaaatact tttgatgaaa gaccgggaaa
     3541 ccaacaaatc aagaggattt gcttttgtta ccttcgaaag cccagcagat gctaaagatg
     3601 cagctagaga tatgaatgga aagtccttgg atggaaaagc catcaaggtg gagcaagcta
     3661 ccaaaccatc ttttgaaagt ggcaggcgtg gaccacctcc acctccaaga agcaggggcc
     3721 ctcccagagg tcttcgagga ggaagtggag gaactagggg acccccttca cgtggaggat
     3781 acatggatga tggtggttat tccatgaact ttaacatgag ttcttccagg ggaccacttc
     3841 cagtaaagag aggaccacca ccacgaagcg ggggtccccc tcctaaaaga tcaacacctt
     3901 ccggaccagt tcgaagcagc agtggaatgg gcggaagaac accagtgtcc cgtggaagag
     3961 atagctacgg gggtccacca cgaagggaac ccctgccatc tcgcagagat gtttatttgt
     4021 ccccaagaga tgatggatat tctacaaaag atagctattc aagcagagat tacccaagct
     4081 cccgagacac cagagattat gcaccgccac caagagatta tacttaccgt gattacagtc
     4141 attccagttc ccgtgatgac tatccatcaa gaggctacgg tgatagagat ggatatggtc
     4201 gggatcgtga ctattcagat catccaagtg gaggttccta cagagattca tatgagagtt
     4261 atggaaactc acgcagtgca ccccctacac gagggccacc accatcttat ggaggaagca
     4321 gtcgctatga tgattacagc agctcacgtg atggctacgg tggaagtcga gacagttact
     4381 caagcagcag aagtgatctc tactcaagtg gccgtgatcg agttggcaga caagaacgag
     4441 ggcttccccc ttctatggaa agggggtacc ctcctccacg tgattcctac agcagttcaa
     4501 gccgaggagc gccaagagga ggtggccgtg gagggagccg atctgataga gggggaggca
     4561 gaagcagata tggtgggccg ggcgggggtg gcgggggagg taagcctatc cctaaccctc
     4621 tcctcggtct cgattctacg tgagtttaaa cttcgaaacg cgtgaattcc tcgagggcgg
     4681 ccgctctaga gtcgacgggc cgcggtaaca attgttaact aacttaagct agcaacggtt
     4741 tccctctagc gggatcaatt ccgccccccc cccctaacgt tactggccga agccgcttgg
     4801 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
     4861 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
     4921 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
     4981 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
     5041 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
     5101 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
     5161 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
     5221 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc
     5281 ccccgaacca cggggacgtg gttttccttt gaaaaacacg ataataccat gaccgagtac
     5341 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     5401 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     5461 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     5521 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     5581 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     5641 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     5701 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     5761 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     5821 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     5881 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacc cgggcggggc
     5941 gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     6001 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     6061 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     6121 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     6181 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     6241 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     6301 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     6361 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     6421 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     6481 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     6541 ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     6601 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     6661 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     6721 ccacttttta aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga
     6781 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac
     6841 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     6901 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
     6961 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg
     7021 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata
     7081 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg
     7141 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg
     7201 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     7261 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     7321 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     7381 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     7441 agagtgagag gccttgacat tgctagcgtt taccgtcgac ctctagctag agcttggcgt
     7501 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     7561 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     7621 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     7681 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     7741 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     7801 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     7861 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     7921 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     7981 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     8041 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     8101 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     8161 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     8221 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     8281 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     8341 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     8401 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     8461 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     8521 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     8581 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     8641 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     8701 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     8761 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     8821 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     8881 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     8941 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     9001 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     9061 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     9121 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     9181 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     9241 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     9301 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     9361 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     9421 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     9481 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     9541 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     9601 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     9661 acgtcgacgg atcgggagat caacttgttt attgcagctt ataatggtta caaataaagc
     9721 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     9781 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa
     9841 aatcatccca aacttcccac cccataccct attaccactg ccaattacct gtggtttcat
     9901 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa
     9961 atatgtacta caaacttagt agt
//
