LOCUS       sequence-35194-e         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Kim do J, Lee SJ, Kim HS, Kim KH, Lee HH, Yoon HJ, Suh SW
  TITLE     Structural basis of octanoic acid recognition by lipoate-protein 
            ligase B.
  JOURNAL   Proteins. 2008 Mar . 70(4):1620-5.
  PUBMED    18076036
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proteins. 
            2008 Mar . 70(4):1620-5."
COMMENT     Sequence Label: pET28b-LipB
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..90
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             754..771
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atattttgtt taactttaag aaggagatat accatgggca gcagccatca tcatcatcat
       61 cacagcagcg gcctggtgcc gcgcggcagc catatggagt tcctggtgga ggacctcggc
      121 ttggtgcctt acggggaggc ctgggcgtac cagaagcggg tgcaccggga ggtggtggcg
      181 ggaaaccgcc cccccaccct cctcctcctg gagcacccca gggtcatcac cttaggccgg
      241 aaggccacgg gggagaacct gctcttcccc gagagctggt accgggagaa cgggtttgag
      301 ctctactggg tggagcgggg cggggacgtg acctaccacg gccccgggca gctggtgggc
      361 taccccatct tccccgtggg ccgggaggtg cgccgctttt taaggcagat tgaggaggcc
      421 atcgtccggg tggccgcggg ctacgggatc tccgcctacc ccaccccggg ctacgccggg
      481 gtctgggtgg gggaggacaa gctctgcgcc atcggcgtgg cggtgaagga gggggtgagc
      541 ttccacggct tcgccctgaa cgtcaacacc gacctcaacg acttcaccgt catcgtcccc
      601 tgcggcctca agggaaaggg ggtcacttcc ttggagaagc ttttgggccg gaaggtcccc
      661 atggaggagg ccaaggccag ggtggtggcg gccttcgccg aggtcttcgg cctgaggccc
      721 gtagagggga gtgtccatga agcctaactc gagcaccacc accaccacca ctgagatccg
      781 gctgctaaca aagccc
//
