LOCUS       sequence-351965-        9432 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Cas9 and sgRNA cassette for targeting RPL13A in yeast 
            Saccharomyces cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Torello Pianale L, Rugbjerg P, Olsson L
  TITLE     Real-Time Monitoring of the Yeast Intracellular State During 
            Bioprocesses With a Toolbox of Biosensors.
  JOURNAL   Front Microbiol. 2022 Jan 7;12:802169. doi: 
            10.3389/fmicb.2021.802169. eCollection 2021.
  PUBMED    35069506
REFERENCE   2  (bases 1 to 9432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2022-01-7"; volume: "12:802169. doi"; pages: " 
            10.3389/fmicb.2021.802169. eCollection 2021"
COMMENT     Sequence Label: YN2_1_LT84_RPL13A
FEATURES             Location/Qualifiers
     source          1..9432
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        305..380
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             1204..5307
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1204..5307
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5320..5340
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5344..5364
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     gene            5695..7050
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     CDS             6039..6848
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(6106..6125)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      6853..7050
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     misc_feature    7055..7558
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     CDS             complement(join(7669..8460,8461..8529))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8292..8311
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8530..8634)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8737..9325
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9226..9245
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccctgaattc gcatctagac tgaatgtgct tcagtattac attttttgcc ttcaacgcct
       61 tgattgttct atttttgcta ataataaatc tatttcatcg gactaaaagt ccattagttg
      121 taagcggatt tagctcagtt gggagagcgc cagactgaag aaaaacttcg gtcaagtcat
      181 ctggaggtcc tgtgttcgat ccacagaatt cgcagatggc cggcatggtc ccagcctcct
      241 cgctggcgcc ggctgggcaa caccttcggg tggcgaatgg gactgaagga aaatacaaaa
      301 attggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa
      361 aagtggcacc gagtcggtgc tttttttatt ttttgtcact attgttatgt aaaatgccac
      421 ctctgacagt atggaacgca aacttctgtc tagtggatac tgatgagacc aaaccagatg
      481 tcaacacagc tacaacggtg agtaaggaaa gagtgaggaa ctatcgcata cctgcattta
      541 aagatgccga tttgggcgcg aatcctttat tttggcttca ccctcatact attatcaggg
      601 ccagaaaaag gaagtgtttc cctccttctt gaattgatgt taccctcata aagcacgtgg
      661 cctcttatcg agaaagaaat taccgtcgct cgtgatttgt ttgcaaaaag aacaaaactg
      721 aaaaaaccca gacacgctcg acttcctgtc atcctattga ttgcagcttc caatttcgtc
      781 acacaacaag gtcctagcga cggctcacag gttttgtaac aagcaatcga aggttctgga
      841 atggcgggaa agggtttagt accacatgct atgatgccca ctgtgatctc cagagcaaag
      901 ttcgttcgat cgtactgtta ctctctctct ttcaaacaga attgtccgaa tcgtgtgaca
      961 acaacagcct gttctcacac actcttttct tctaaccaag ggggtggttt agtttagtag
     1021 aacctcgtga aacttacatt tacatatata taaacttgca taaattggtc aatgcaagaa
     1081 atacatattt ggtcttttct aattcgtagt ttttcaagtt cttagatgct ttctttttct
     1141 cttttttaca gatcatcaag gaagtaatta tctacttttt acaacaaata taaaacaaga
     1201 tctatggaca agaagtattc tatcggactg gatatcggga ctaatagcgt cgggtgggcc
     1261 gtgatcactg acgagtacaa ggtgccctct aagaagttca aggtgctcgg gaacaccgac
     1321 cggcattcca tcaagaaaaa tctgatcgga gctctcctct ttgattcagg ggaaaccgct
     1381 gaagcaaccc gcctcaagcg gactgctaga cggcggtaca ccaggaggaa gaaccggatt
     1441 tgttaccttc aagagatatt ctccaacgaa atggcaaagg tcgacgacag cttcttccat
     1501 aggctggaag aatcattcct cgtggaagag gataagaagc atgaacggca tcccatcttc
     1561 ggtaatatcg tcgacgaggt ggcctatcac gagaaatacc caaccatcta ccatcttcgc
     1621 aaaaagctgg tggactcaac cgacaaggca gacctccggc ttatctacct ggccctggcc
     1681 cacatgatca agttcagagg ccacttcctg atcgagggcg acctcaatcc tgacaatagc
     1741 gatgtggata aactgttcat ccagctggtg cagacttaca accagctctt tgaagagaac
     1801 cccatcaatg caagcggagt cgatgccaag gccattctgt cagcccggct gtcaaagagc
     1861 cgcagacttg agaatcttat cgctcagctg ccgggtgaaa agaaaaatgg actgttcggg
     1921 aacctgattg ctctttcact tgggctgact cccaatttca agtctaattt cgacctggca
     1981 gaggatgcca agctgcaact gtccaaggac acctatgatg acgatctcga caacctcctg
     2041 gcccagatcg gtgaccaata cgccgacctt ttccttgctg ctaagaatct ttctgacgcc
     2101 atcctgctgt ctgacattct ccgcgtgaac actgaaatca ccaaggcccc tctttcagct
     2161 tcaatgatta agcggtatga tgagcaccac caggacctga ccctgcttaa ggcactcgtc
     2221 cggcagcagc ttccggagaa gtacaaggaa atcttctttg accagtcaaa gaatggatac
     2281 gccggctaca tcgacggagg tgcctcccaa gaggaatttt ataagtttat caaacctatc
     2341 cttgagaaga tggacggcac cgaagagctc ctcgtgaaac tgaatcggga ggatctgctg
     2401 cggaagcagc gcactttcga caatgggagc attccccacc agatccatct tggggagctt
     2461 cacgccatcc ttcggcgcca agaggacttc tacccctttc ttaaggacaa cagggagaag
     2521 attgagaaaa ttctcacttt ccgcatcccc tactacgtgg gacccctcgc cagaggaaat
     2581 agccggtttg cttggatgac cagaaagtca gaagaaacta tcactccctg gaacttcgaa
     2641 gaggtggtgg acaagggagc cagcgctcag tcattcatcg aacggatgac taacttcgat
     2701 aagaacctcc ccaatgagaa ggtcctgccg aaacattccc tgctctacga gtactttacc
     2761 gtgtacaacg agctgaccaa ggtgaaatat gtcaccgaag ggatgaggaa gcccgcattc
     2821 ctgtcaggcg aacaaaagaa ggcaattgtg gaccttctgt tcaagaccaa tagaaaggtg
     2881 accgtgaagc agctgaagga ggactatttc aagaaaattg aatgcttcga ctctgtggag
     2941 attagcgggg tcgaagatcg gttcaacgca agcctgggta cttaccatga tctgcttaag
     3001 atcatcaagg acaaggattt tctggacaat gaggagaacg aggacatcct tgaggacatt
     3061 gtcctgactc tcactctgtt cgaggaccgg gaaatgatcg aggagaggct taagacctac
     3121 gcccatctgt tcgacgataa agtgatgaag caacttaaac ggagaagata taccggatgg
     3181 ggacgcctta gccgcaaact catcaacgga atccgggaca aacagagcgg aaagaccatt
     3241 cttgatttcc ttaagagcga cggattcgct aatcgcaact tcatgcaact tatccatgat
     3301 gattccctga cctttaagga ggacatccag aaggcccaag tgtctggaca aggtgactca
     3361 ctgcacgagc atatcgcaaa tctggctggt tcacccgcta ttaagaaggg tattctccag
     3421 accgtgaaag tcgtggacga gctggtcaag gtgatgggtc gccataaacc agagaacatt
     3481 gtcatcgaga tggccaggga aaaccagact acccagaagg gacagaagaa cagcagggag
     3541 cggatgaaaa gaattgagga agggattaag gagctcgggt cacagatcct taaagagcac
     3601 ccggtggaaa acacccagct tcagaatgag aagctctatc tgtactacct tcaaaatgga
     3661 cgcgatatgt atgtggacca agagcttgat atcaacaggc tctcagacta cgacgtggac
     3721 catatcgtcc ctcagagctt cctcaaagac gactcaattg acaataaggt gctgactcgc
     3781 tcagacaaga accggggaaa gtcagataac gtgccctcag aggaagtcgt gaaaaagatg
     3841 aagaactatt ggcgccagct tctgaacgca aagctgatca ctcagcggaa gttcgacaat
     3901 ctcactaagg ctgagagggg cggactgagc gaactggaca aagcaggatt cattaaacgg
     3961 caacttgtgg agactcggca gattactaaa catgtcgccc aaatccttga ctcacgcatg
     4021 aataccaagt acgacgaaaa cgacaaactt atccgcgagg tgaaggtgat taccctgaag
     4081 tccaagctgg tcagcgattt cagaaaggac tttcaattct acaaagtgcg ggagatcaat
     4141 aactatcatc atgctcatga cgcatatctg aatgccgtgg tgggaaccgc cctgatcaag
     4201 aagtacccaa agctggaaag cgagttcgtg tacggagact acaaggtcta cgacgtgcgc
     4261 aagatgattg ccaaatctga gcaggagatc ggaaaggcca ccgcaaagta cttcttctac
     4321 agcaacatca tgaatttctt caagaccgaa atcacccttg caaacggtga gatccggaag
     4381 aggccgctca tcgagactaa tggggagact ggcgaaatcg tgtgggacaa gggcagagat
     4441 ttcgctaccg tgcgcaaagt gctttctatg cctcaagtga acatcgtgaa gaaaaccgag
     4501 gtgcaaaccg gaggcttttc taaggaatca atcctcccca agcgcaactc cgacaagctc
     4561 attgcaagga agaaggattg ggaccctaag aagtacggcg gattcgattc accaactgtg
     4621 gcttattctg tcctggtcgt ggctaaggtg gaaaaaggaa agtctaagaa gctcaagagc
     4681 gtgaaggaac tgctgggtat caccattatg gagcgcagct ccttcgagaa gaacccaatt
     4741 gactttctcg aagccaaagg ttacaaggaa gtcaagaagg accttatcat caagctccca
     4801 aagtatagcc tgttcgaact ggagaatggg cggaagcgga tgctcgcctc cgctggcgaa
     4861 cttcagaagg gtaatgagct ggctctcccc tccaagtacg tgaatttcct ctaccttgca
     4921 agccattacg agaagctgaa ggggagcccc gaggacaacg agcaaaagca actgtttgtg
     4981 gagcagcata agcattatct ggacgagatc attgagcaga tttccgagtt ttctaaacgc
     5041 gtcattctcg ctgatgccaa cctcgataaa gtccttagcg catacaataa gcacagagac
     5101 aaaccaattc gggagcaggc tgagaatatc atccacctgt tcaccctcac caatcttggt
     5161 gcccctgccg cattcaagta cttcgacacc accatcgacc ggaaacgcta tacctccacc
     5221 aaagaagtgc tggacgccac cctcatccac cagagcatca ccggacttta cgaaactcgg
     5281 attgacctct cacagctcgg aggggatgag ggagctgatc caaaaaagaa gagaaaggta
     5341 gatccaaaaa agaagagaaa ggtataagga tcctaactcg agattgaatt gaattgaaat
     5401 cgatagatca atttttttct tttctctttc cccatccttt acgctaaaat aatagtttat
     5461 tttatttttt gaatattttt tatttatata cgtatatata gactattatt tatcttttaa
     5521 tgattattaa gatttttatt aaaaaaaaat tcgctcctct tttaatgcct ttatgcagtt
     5581 tttttttccc attcgatatt tctatgtgct gagcagttac agagatgtta cgaaccacta
     5641 gtgcactgca gtacagttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg
     5701 gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac
     5761 tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc catacatttt
     5821 gatggccgca cggcgcgaag caaaaattac ggctcctcgc tccagacctg cgagcaggga
     5881 aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata
     5941 aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta
     6001 ggatacagtt ctcacatcac atccgaacat aaacaaaaat gggtaaggaa aagactcacg
     6061 tttcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc
     6121 gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc
     6181 cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg
     6241 tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta
     6301 ctcctgatga tgcatggtta ctcaccactg cgatccccgg caaaacagca ttccaggtat
     6361 tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc
     6421 ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctgg
     6481 ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc
     6541 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taagcttttg ccattctcac
     6601 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga
     6661 aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg
     6721 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa
     6781 aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt
     6841 ttttctaaag taactgacaa taaaaagatt cttgttttca agaacttgtc atttgtatag
     6901 tttttttata ttgtagttgt tctattttaa tcaaatgtta gcgtgattta tatttttttt
     6961 cgcctcgaca tcatctgccc agatgcgaag ttaagtgcgc agaaagtaat atcatgcgtc
     7021 aatcgtatgt gaatgctggt cgctatactg gagtatcacg tgctataaaa ataattataa
     7081 tttaaatttt ttaatataaa tatataaatt aaaaatagaa agtaaaaaaa gaaattaaag
     7141 aaaaaatagt ttttgttttc cgaagatgta aaagactcta gggggatcgc caacaaatac
     7201 taccttttat cttgctcttc ctgctctcag gtattaatgc cgaattgttt catcttgtct
     7261 gtgtagaaaa ccacacacga aaatcctgtg attttacatt ttacttatcg ttaatcgaat
     7321 gtatatctat ttaatctgct tttcttgtct aataaatata tatgtaaagt acgctttttg
     7381 ttgaaatttt ttaaaccttt gtttattttt ttttcttcat tccgtaactc ttctaccttc
     7441 tttatttact ttctaaaatc caaatacaaa acataaaaat aaataaacac agagtaaatt
     7501 cccaaattat tccatcatta aaagatacga ggcgcgtgta agttacaggc aagcgatccg
     7561 tcccgagcgg ccgcgattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     7621 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7681 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7741 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7801 gataccgcgg gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7861 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     7921 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     7981 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     8041 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     8101 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     8161 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     8221 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     8281 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8341 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     8401 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     8461 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     8521 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     8581 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     8641 tccccgaaaa gtgccacctg tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     8701 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     8761 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     8821 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     8881 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     8941 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     9001 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     9061 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     9121 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     9181 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     9241 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     9301 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     9361 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     9421 ccgtgcggcc gc
//
