LOCUS       sequence-351967-        4590 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for genome integration in X2 site expressing QUEEN-2m (ATP 
            biosensor).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Torello Pianale L, Rugbjerg P, Olsson L
  TITLE     Real-Time Monitoring of the Yeast Intracellular State During 
            Bioprocesses With a Toolbox of Biosensors.
  JOURNAL   Front Microbiol. 2022 Jan 7;12:802169. doi: 
            10.3389/fmicb.2021.802169. eCollection 2021.
  PUBMED    35069506
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2022-01-7"; volume: "12:802169. doi"; pages: " 
            10.3389/fmicb.2021.802169. eCollection 2021"
COMMENT     Sequence Label: LT1_36_QUEEN-2m
FEATURES             Location/Qualifiers
     source          1..4590
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        472..885
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             1262..1513
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     1450..1471
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1577..1598)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1838..1857)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(2062..2078)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2063..2079)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(2097..2115)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(join(2723..3514,3515..3583))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3346..3365
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3584..3688)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3791..4379
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4280..4299
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccctgaattc gcatctagat ggtagagcca caaacagccg gtacaagcaa cgatctccag
       61 gaccatctga atcatgcgcg gatgacacga actcacgacg gcgatcacag acattaaccc
      121 acagtacaga cactgcgaca acgtggcaat tcgtcgcaat accgtctcac tgaactggcc
      181 gataattgca gacgaacgcc ttgccaacag ggagttcttc agagacatgg aggctcaaaa
      241 cgaaattatt gacagcctag acatcaatag tcatacaaca gaaagcgacc acccaacttt
      301 ggctgataat agcgtataaa caatgcatac tttgtacgtt caaaatacaa tgcagtagat
      361 atatttatgc atattacata taatacatat cacataggaa gcaacaggcg cgttggactt
      421 ttaattttcg aggaccgcga atccttacat cacacccaat cccccacaag tgatccccca
      481 cacaccatag cttcaaaatg tttctactcc ttttttactc ttccagattt tctcggactc
      541 cgcgcatcgc cgtaccactt caaaacaccc aagcacagca tactaaattt cccctctttc
      601 ttcctctagg gtgtcgttaa ttacccgtac taaaggtttg gaaaagaaaa aagacaccgc
      661 ctcgtttctt tttcttcgtc gaaaaaggca ataaaaattt ttatcacgtt tctttttctt
      721 gaaaattttt ttttttgatt tttttctctt tcgatgacct cccattgata tttaagttaa
      781 taaacggtca tcaatttctc aagtttcagt ttcatttttc ttgttctatt acaacttttt
      841 ttacttcttg ctcattagaa agaaagcata gcaatctaat ctaagtttta attacaaaag
      901 atctatgaaa actgtgaaag tgaatataac aacccctgat gggccagtct acgacgctga
      961 tatcgagatg gtgtccgtgc gggccgagag tggtgatctc ggcatcctcc ccggtcacat
     1021 tcccacaaag gccccactga agatcggagc tgtgcggctg aagaaggacg gccaaaccga
     1081 gatggtcgca gtctcaggcg gcactgttga agtgcggcct gaccacgtta ccattaatgc
     1141 tcaagccgct gaaacagccg aaggaatcga caaagagaga gcagaagccg caagacagag
     1201 ggcccaggag cggctgaact ctcaaacccg ttacaacagc cacaacgtct atatcatggc
     1261 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     1321 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     1381 gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa
     1441 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     1501 cgagctgtac aagggtggca ccggagggag catggtgagc aagggcgagg agctgttcac
     1561 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     1621 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     1681 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     1741 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     1801 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     1861 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     1921 cttcaaggag gacggcaaca tcctggggca caagctggag tacaacctgg gtgacatcga
     1981 cttcaaacgg gccgaactgg cgttaaaacg cgccatgaac cgtttgagcg ttgcggaaat
     2041 gaagggatcc taactcgaga tcgataccgt cgacctcgag tcatgtaatt agttatgtca
     2101 cgcttacatt cacgccctcc tcccacatcc gctctaaccg aaaaggaagg agttagacaa
     2161 cctgaagtct aggtccctat ttattttttt taatagttat gttagtatta agaacgttat
     2221 ttatatttca aatttttctt ttttttctgt acaaacgcgt gtacgcatgt aacattatac
     2281 tgaaaacctt gcttgagaag gttttgggac gctcggctgc caatgagacg acggggtcat
     2341 cacggctcat catgcgccaa acaaatgtgt gcaatacacg ctcggatgac tgcatgatga
     2401 ccgcactgac tggggacagc agatccacct aagcctgtga gagaagcaga cacccgacag
     2461 atcaaggcag ttaactagtg cactgcagta cataacccct aggtcttcct gagagtgacg
     2521 aaggctaagg tcacttcttc gtttccttta ttggggtttc cgtgtagcct tcccctgaat
     2581 agtgtgggac gttttatgag aagccgtaag aaatagccga gcggccgcga ttatcaaaaa
     2641 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     2701 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     2761 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     2821 gggagggctt accatctggc cccagtgctg caatgatacc gcgggaccca cgctcaccgg
     2881 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     2941 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     3001 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     3061 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     3121 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     3181 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     3241 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     3301 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     3361 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     3421 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     3481 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     3541 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     3601 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     3661 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgtcatga
     3721 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     3781 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3841 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3901 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     3961 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     4021 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     4081 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     4141 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     4201 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4261 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4321 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4381 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     4441 tctttcctgc gttatcccct gattctgtgg ataaccgtgc ggccgccaat gtcaaaagat
     4501 cctctcatac catattaagt aaattgcctc catttctttt tcctcgggca gagaaactcg
     4561 caggcaactt gctctcgaag tggtcacgtg
//
