LOCUS       sequence-351979-       14633 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For making Rabies virus SAD B19 strain L cell line with hyPBase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14633)
  AUTHORS   Jin L, Sullivan HA, Zhu M, Lavin TK, Matsuyama M, Fu X, Lea NE, Xu 
            R, Hou Y, Rutigliani L, Pruner M, Babcock KR, Ip JPK, Hu M, Daigle 
            TL, Zeng H, Sur M, Feng G, Wickersham IR
  TITLE     Long-term labeling and imaging of synaptically connected neuronal 
            networks in vivo using double-deletion-mutant rabies viruses.
  JOURNAL   Nat Neurosci. 2024 Jan 11. doi: 10.1038/s41593-023-01545-8.
  PUBMED    38212587
REFERENCE   2  (bases 1 to 14633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2024 Jan 11. doi: 10.1038/s41593-023-01545-8."
COMMENT     Sequence Label: pB-CAG-B19L-IRES-mCherry-WPRE-BGHpA
FEATURES             Location/Qualifiers
     source          1..14633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          127..1143
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1067..1089
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1151..1170
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2852..2863
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    7623..8174
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7767..7784)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7994..8013
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8186..8896
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8331..8349)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8599..8618
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    8902..9490
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8955..8975)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9373..9384)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    9494..9718
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   10249..10283
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(10774..10790)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10774..10790)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10787..10809)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10798..10814
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10822..10828,10829..10846,10847..10852))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10867..10888
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11005..11022)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11176..11764)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11256..11275)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11935..12726,12727..12795))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12558..12577
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12796..12900)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(12926..13381)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13063..13084)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     13275..13294
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13508..13530
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     13522..13539
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     13523..13539
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   13669..13731
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        14214..14517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga
       61 aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg
      121 cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc
      181 cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct
      241 tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg
      301 cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt
      361 gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt
      421 gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg
      481 gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg
      541 ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc
      601 ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg
      661 gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc
      721 ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg cccccggagc
      781 gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag
      841 agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc
      901 cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc
      961 ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc
     1021 tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg
     1081 cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta
     1141 cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattccgc
     1201 cgccaccatg ctcgatcctg gagaggtcta tgatgaccct attgacccaa tcgagttaga
     1261 ggctgaaccc agaggaaccc ccattgtccc caacatcttg aggaactctg actacaatct
     1321 caactctcct ttgatagaag atcctgctag actaatgtta gaatggttaa aaacagggaa
     1381 tagaccttat cggatgactc taacagacaa ttgctccagg tctttcagag ttttgaaaga
     1441 ttatttcaag aaggtagatt tgggttctct caaggtgggc ggaatggctg cacagtcaat
     1501 gatttctctc tggttatatg gtgcccactc tgaatccaac aggagccgga gatgtataac
     1561 agacttggcc catttctatt ccaagtcgtc ccccatagag aagctgttga atctcacgct
     1621 aggaaataga gggctgagaa tccccccaga gggagtgtta agttgccttg agagggttga
     1681 ttatgataat gcatttggaa ggtatcttgc caacacgtat tcctcttact tgttcttcca
     1741 tgtaatcacc ttatacatga acgccctaga ctgggatgaa gaaaagacca tcctagcatt
     1801 atggaaagat ttaacctcag tggacatcgg gaaggacttg gtaaagttca aagaccaaat
     1861 atggggactg ctgatcgtga caaaggactt tgtttactcc caaagttcca attgtctttt
     1921 tgacagaaac tacacactta tgctaaaaga tcttttcttg tctcgcttca actccttaat
     1981 ggtcttgctc tctcccccag agccccgata ctcagatgac ttgatatctc aactatgcca
     2041 gctgtacatt gctggggatc aagtcttgtc tatgtgtgga aactccggct atgaagtcat
     2101 caaaatattg gagccatatg tcgtgaatag tttagtccag agagcagaaa agtttaggcc
     2161 tctcattcat tccttgggag actttcctgt atttataaaa gacaaggtaa gtcaacttga
     2221 agagacgttc ggtccctgtg caagaaggtt ctttagggct ctggatcaat tcgacaacat
     2281 acatgacttg gtttttgtgt ttggctgtta caggcattgg gggcacccat atatagatta
     2341 tcgaaagggt ctgtcaaaac tatatgatca ggttcacctt aaaaaaatga tagataagtc
     2401 ctaccaggag tgcttagcaa gcgacctagc caggaggatc cttagatggg gttttgataa
     2461 gtactccaag tggtatctgg attcaagatt cctagcccga gaccacccct tgactcctta
     2521 tatcaaaacc caaacatggc cacccaaaca tattgtagac ttggtggggg atacatggca
     2581 caagctcccg atcacgcaga tctttgagat tcctgaatca atggatccgt cagaaatatt
     2641 ggatgacaaa tcacattctt tcaccagaac gagactagct tcttggctgt cagaaaaccg
     2701 aggggggcct gttcctagcg aaaaagttat tatcacggcc ctgtctaagc cgcctgtcaa
     2761 tccccgagag tttctgaggt ctatagacct cggaggattg ccagatgaag acttgataat
     2821 tggcctcaag ccaaaggaac gggaattgaa gattgaaggt cgattctttg ctctaatgtc
     2881 atggaatcta agattgtatt ttgtcatcac tgaaaaactc ttggccaact acatcttgcc
     2941 actttttgac gcgctgacta tgacagacaa cctgaacaag gtgtttaaaa agctgatcga
     3001 cagggtcacc gggcaagggc ttttggacta ttcaagggtc acatatgcat ttcacctgga
     3061 ctatgaaaag tggaacaacc atcaaagatt agagtcaaca gaggatgtat tttctgtcct
     3121 agatcaagtg tttggattga agagagtgtt ttctagaaca cacgagtttt ttcaaaaggc
     3181 ctggatctat tattcagaca gatcagacct catcgggtta cgggaggatc aaatatactg
     3241 cttagatgcg tccaacggcc caacctgttg gaatggccag gatggcgggc tagaaggctt
     3301 acggcagaag ggctggagtc tagtcagctt attgatgata gatagagaat ctcaaatcag
     3361 gaacacaaga accaaaatac tagctcaagg agacaaccag gttttatgtc cgacatacat
     3421 gttgtcgcca gggctatctc aagaggggct cctctatgaa ttggagagaa tatcaaggaa
     3481 tgcactttcg atatacagag ccgtcgagga aggggcatct aagctagggc tgatcatcaa
     3541 gaaagaagag accatgtgta gttatgactt cctcatctat ggaaaaaccc ctttgtttag
     3601 aggtaacata ttggtgcctg agtccaaaag atgggccaga gtctcttgcg tctctaatga
     3661 ccaaatagtc aacctcgcca atataatgtc gacagtgtcc accaatgcgc taacagtggc
     3721 acaacactct caatctttga tcaaaccgat gagggatttt ctgctcatgt cagtacaggc
     3781 agtctttcac tacctgctat ttagcccaat cttaaaggga agagtttaca agattctgag
     3841 cgctgaaggg gagagctttc tcctagccat gtcaaggata atctatctag atccttcttt
     3901 gggagggata tctggaatgt ccctcggaag attccatata cgacagttct cagaccctgt
     3961 ctctgaaggg ttatccttct ggagagagat ctggttaagc tcccaagagt cctggattca
     4021 cgcgttgtgt caagaggctg gaaacccaga tcttggagag agaacactcg agagcttcac
     4081 tcgccttcta gaagatccga ccaccttaaa tatcagagga ggggccagtc ctaccattct
     4141 actcaaggat gcaatcagaa aggctttata tgacgaggtg gacaaggtgg aaaattcaga
     4201 gtttcgagag gcaatcctgt tgtccaagac ccatagagat aattttatac tcttcttaat
     4261 atctgttgag cctctgtttc ctcgatttct cagtgagcta ttcagttcgt cttttttggg
     4321 aatccccgag tcaatcattg gattgataca aaactcccga acgataagaa ggcagtttag
     4381 aaagagtctc tcaaaaactt tagaagaatc cttctacaac tcagagatcc acgggattag
     4441 tcggatgacc cagacacctc agagggttgg gggggtgtgg ccttgctctt cagagagggc
     4501 agatctactt agggagatct cttggggaag aaaagtggta ggcacgacag ttcctcaccc
     4561 ttctgagatg ttgggattac ttcccaagtc ctctatttct tgcacttgtg gagcaacagg
     4621 aggaggcaat cctagagttt ctgtatcagt actcccgtcc tttgatcagt catttttttc
     4681 acgaggcccc ctaaagggat acttgggctc gtccacctct atgtcgaccc agctattcca
     4741 tgcatgggaa aaagtcacta atgttcatgt ggtgaagaga gctctatcgt taaaagaatc
     4801 tataaactgg ttcattacta gagattccaa cttggctcaa gctctaatta ggaacattat
     4861 gtctctgaca ggccctgatt tccctctaga ggaggcccct gtcttcaaaa ggacggggtc
     4921 agccttgcat aggttcaagt ctgccagata cagcgaagga gggtattctt ctgtctgccc
     4981 gaacctcctc tctcatattt ctgttagtac agacaccatg tctgatttga cccaagacgg
     5041 gaagaactac gatttcatgt tccagccatt gatgctttat gcacagacat ggacatcaga
     5101 gctggtacag agagacacaa ggctaagaga ctctacgttt cattggcacc tccgatgcaa
     5161 caggtgtgtg agacccattg acgacgtgac cctggagacc tctcagatct tcgagtttcc
     5221 ggatgtgtcg aaaagaatat ccagaatggt ttctggggct gtgcctcact tccagaggct
     5281 tcccgatatc cgtctgagac caggagattt tgaatctcta agcggtagag aaaagtctca
     5341 ccatatcgga tcagctcagg ggctcttata ctcaatctta gtggcaattc acgactcagg
     5401 atacaatgat ggaaccatct tccctgtcaa catatacggc aaggtttccc ctagagacta
     5461 tttgagaggg ctcgcaaggg gagtattgat aggatcctcg atttgcttct tgacaagaat
     5521 gacaaatatc aatattaata gacctcttga attggtctca ggggtaatct catatattct
     5581 cctgaggcta gataaccatc cctccttgta cataatgctc agagaaccgt ctcttagagg
     5641 agagatattt tctatccctc agaaaatccc cgccgcttat ccaaccacta tgaaagaagg
     5701 caacagatca atcttgtgtt atctccaaca tgtgctacgc tatgagcgag agataatcac
     5761 ggcgtctcca gagaatgact ggctatggat cttttcagac tttagaagtg ccaaaatgac
     5821 gtacctatcc ctcattactt accagtctca tcttctactc cagagggttg agagaaacct
     5881 atctaagagt atgagagata acctgcgaca attgagttct ttgatgaggc aggtgctggg
     5941 cgggcacgga gaagatacct tagagtcaga cgacaacatt caacgactgc taaaagactc
     6001 tttacgaagg acaagatggg tggatcaaga ggtgcgccat gcagctagaa ccatgactgg
     6061 agattacagc cccaacaaga aggtgtcccg taaggtagga tgttcagaat gggtctgctc
     6121 tgctcaacag gttgcagtct ctacctcagc aaacccggcc cctgtctcgg agcttgacat
     6181 aagggccctc tctaagaggt tccagaaccc tttgatctcg ggcttgagag tggttcagtg
     6241 ggcaaccggt gctcattata agcttaagcc tattctagat gatctcaatg ttttcccatc
     6301 tctctgcctt gtagttgggg acgggtcagg ggggatatca agggcagtcc tcaacatgtt
     6361 tccagatgcc aagcttgtgt tcaacagtct tttagaggtg aatgacctga tggcttccgg
     6421 aacacatcca ctgcctcctt cagcaatcat gaggggagga aatgatatcg tctccagagt
     6481 gatagatctt gactcaatct gggaaaaacc gtccgacttg agaaacttgg caacctggaa
     6541 atacttccag tcagtccaaa agcaggtcaa catgtcctat gacctcatta tttgcgatgc
     6601 agaagttact gacattgcat ctatcaaccg gatcaccctg ttaatgtccg attttgcatt
     6661 gtctatagat ggaccactct atttggtctt caaaacttat gggactatgc tagtaaatcc
     6721 aaactacaag gctattcaac acctgtcaag agcgttcccc tcggtcacag ggtttatcac
     6781 ccaagtaact tcgtcttttt catctgagct ctacctccga ttctccaaac gagggaagtt
     6841 tttcagagat gctgagtact tgacctcttc cacccttcga gaaatgagcc ttgtgttatt
     6901 caattgtagc agccccaaga gtgagatgca gagagctcgt tccttgaact atcaggatct
     6961 tgtgagagga tttcctgaag aaatcatatc aaatccttac aatgagatga tcataactct
     7021 gattgacagt gatgtagaat cttttctagt ccacaagatg gttgatgatc ttgagttaca
     7081 gaggggaact ctgtctaaag tggctatcat tatagccatc atgatagttt tctccaacag
     7141 agtcttcaac gtttccaaac ccctaactga cccctcgttc tatccaccgt ctgatcccaa
     7201 aatcctgagg cacttcaaca tatgttgcag tactatgatg tatctatcta ctgctttagg
     7261 tgacgtccct agcttcgcaa gacttcacga cctgtataac agacctataa cttattactt
     7321 cagaaagcaa gtcattcgag ggaacgttta tctatcttgg agttggtcca acgacacctc
     7381 agtgttcaaa agggtagcct gtaattctag cctgagtctg tcatctcact ggatcaggtt
     7441 gatttacaag atagtgaaga ctaccagact cgttggcagc atcaaggatc tatccagaga
     7501 agtggaaaga caccttcata ggtacaacag gtggatcacc ctagaggata tcagatctag
     7561 atcatcccta ctagactaca gttgcctgtg aaattcgaat ttaaatcgga tcgcggccgc
     7621 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
     7681 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     7741 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
     7801 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     7861 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     7921 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     7981 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     8041 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
     8101 gtcgaggtta aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     8161 acacgatgat aatatggcca caaccatggt gagcaagggc gaggaggata acatggccat
     8221 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
     8281 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     8341 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
     8401 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
     8461 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
     8521 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
     8581 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
     8641 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
     8701 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
     8761 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
     8821 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactctaccg gcggcatgga
     8881 cgagctgtac aagtaagatc caatcaacct ctggattaca aaatttgtga aagattgact
     8941 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     9001 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     9061 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     9121 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     9181 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     9241 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     9301 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     9361 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     9421 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     9481 gcctccccgc ctgctgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     9541 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     9601 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     9661 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggag
     9721 atccgcggcc cattggtatg gctttttccc cgtatccccc caggtgtctg caggctcaaa
     9781 gagcagcgag aagcgttcag aggaaagcga tcccgtgcca ccttccccgt gcccgggctg
     9841 tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg
     9901 gcggctcgct gctgccccct agcgggggag ggacgtaatt acatccctgg gggctttggg
     9961 ggggggctgt ccctgatatc tataacaaga aaatatatat ataataagtt atcacgtaag
    10021 tagaacatga aataacaata taattatcgt atgagttaaa tcttaaaagt cacgtaaaag
    10081 ataatcatgc gtcattttga ctcacgcggt cgttatagtt caaaatcagt gacacttacc
    10141 gcattgacaa gcacgcctca cgggagctcc aagcggcgac tgagatgtcc taaatgcaca
    10201 gcgacggatt cgcgctattt agaaagagag agcaatattt caagaatgca tgcgtcaatt
    10261 ttacgcagac tatctttcta gggttaatct agctgcatca ggatcatatc gtcgggtctt
    10321 ttttccggct cagtcatcgc ccaagctggc gctatctggg catcggggag gaagaagccc
    10381 gtgccttttc ccgcgaggtt gaagcggcat ggaaagagtt tgccgaggat gactgctgct
    10441 gcattgacgt tgagcgaaaa cgcacgttta ccatgatgat tcgggaaggt gtggccatgc
    10501 acgcctttaa cggtgaactg ttcgttcagg ccacctggga taccagttcg tcgcggcttt
    10561 tccggacaca gttccggatg gtcagcccga agcgcatcag caacccgaac aataccggcg
    10621 acagccggaa ctgccgtgcc ggtgtgcaga ttaatgacag cggtgcggcg ctgggatatt
    10681 acgtcagcga ggacgggtat cctggctgga tgccgcagaa atggacatgg ataccccgtg
    10741 agttacccgg cgggcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
    10801 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
    10861 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
    10921 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
    10981 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
    11041 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
    11101 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
    11161 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
    11221 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
    11281 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
    11341 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
    11401 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
    11461 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
    11521 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
    11581 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
    11641 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
    11701 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
    11761 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    11821 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
    11881 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
    11941 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    12001 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    12061 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    12121 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    12181 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    12241 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    12301 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    12361 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    12421 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    12481 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    12541 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    12601 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    12661 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    12721 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    12781 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    12841 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    12901 cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg
    12961 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
    13021 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
    13081 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
    13141 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
    13201 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
    13261 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
    13321 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
    13381 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
    13441 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
    13501 gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcctc gttcattcac
    13561 gtttttgaac ccgtggagga cgggcagact cgcggtgcaa atgtgtttta cagcgtgatg
    13621 gagcagatga agatgctcga cacgctgcag aacacgcagc tagattaacc ctagaaagat
    13681 aatcatattg tgacgtacgt taaagataat catgtgtaaa attgacgcat gtgttttatc
    13741 ggtctgtata tcgaggttta tttattaatt tgaatagata ttaagtttta ttatatttac
    13801 acttacatac taataataaa ttcaacaaac aatttattta tgtttattta tttattaaaa
    13861 aaaacaaaaa ctcaaaattt cttctataaa gtaacaaaac ttttatgagg gacagccccc
    13921 ccccaaagcc cccagggatg taattacgtc cctcccccgc tagggggcag cagcgagccg
    13981 cccggggctc cgctccggtc cggcgctccc cccgcatccc cgagccggca gcgtgcgggg
    14041 acagcccggg cacggggaag gtggcacggg atcgctttcc tctgaacgct tctcgctgct
    14101 ctttgagcct gcagacacct ggggggatac ggggaaaagg cctccacggc cactagttat
    14161 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
    14221 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
    14281 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
    14341 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
    14401 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
    14461 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
    14521 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
    14581 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg ggg
//
