LOCUS       sequence-351985-       17324 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For making TRE-CB mouse line in Tigre locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17324)
  AUTHORS   Jin L, Sullivan HA, Zhu M, Lavin TK, Matsuyama M, Fu X, Lea NE, Xu 
            R, Hou Y, Rutigliani L, Pruner M, Babcock KR, Ip JPK, Hu M, Daigle 
            TL, Zeng H, Sur M, Feng G, Wickersham IR
  TITLE     Long-term labeling and imaging of synaptically connected neuronal 
            networks in vivo using double-deletion-mutant rabies viruses.
  JOURNAL   Nat Neurosci. 2024 Jan 11. doi: 10.1038/s41593-023-01545-8.
  PUBMED    38212587
REFERENCE   2  (bases 1 to 17324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2024 Jan 11. doi: 10.1038/s41593-023-01545-8."
COMMENT     Sequence Label: TRE-mCardinal-P2A-B19L Flp-in vector
FEATURES             Location/Qualifiers
     source          1..17324
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    414..447
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     promoter        3009..3323
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    3017..3035
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3053..3071
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3088..3106
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3124..3142
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3160..3178
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3195..3213
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3231..3249
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3296..3320
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3360..3369
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3366..4097
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     CDS             4107..4163
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5808..5819
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    10572..11160
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10625..10645)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11043..11054)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(11185..11202)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11191..11398
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    13896..13929
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        13956..14455
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(13991..14014)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     14388..14407
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(15103..15119)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15103..15119)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(15116..15138)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    15127..15143
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(15151..15157,15158..15175,15176..15181))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15196..15217
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(15334..15351)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15505..16093)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15585..15604)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(16264..17055,17056..17124))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16887..16906
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(17125..17229)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     17297..17315
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt accgaagttc
      421 ctattcttca aatagtatag gaacttcttt cggccggcca atcgattgga tccgttttat
      481 gccgcggtta ccgtgaagat atcttaatta agaattcgag ctcgctagag ccccatcctc
      541 actgactccg tcctggagtt ggatgagaga taatggcctt acgttgtgcc aggggagggt
      601 cgggctggat ttagcaagat ttaccttctc caaagagcgg tgctgcagtg gcacagctgc
      661 ccacggaggt gggggggtca ccgtccctgg aggtgatgaa gaactgtggg gatgtggcac
      721 tgagggacat ggccagtggg cacggtgggt gggttggggt tggtcttggg gatcttggag
      781 ggcttttcca gccttcatga tttgacgatt gtatgaacat ctacatggca attctccagc
      841 tgcctgtccc agtcctactg acccagctgt atctctccag gcaagctctt ccaccccttc
      901 tgcttgcatc cagacaccat caaacatgca ggctcagaca cagggaccag cagtgtctgt
      961 ggcctttttg tgctcctctc catgctgggt tttaacttgc tctttgtcct tctatcctat
     1021 cttcttatcc ttaaggctgt tctgaacgct gtgacttgga gagtgtccca gagccctcaa
     1081 cacctgcatg tcccacgtcc atgctgtcct gcacttcctt atccccaaga tctgcctctc
     1141 cgtgatgcac tgaattggca aacatgtgtc accccagacc aacaatgtca cagcaaactc
     1201 ccccttgata ggacaagggg gaatggcttt acactgagac aggggaggtt tgggttggat
     1261 atgaggaggc agtttttccc ccagagggtg gtgacgcact gaacaggttg cccaaggagg
     1321 ctgtggatgc cccatccctg caggcattca aggccaggct ggatgtggct ctgggcagcc
     1381 tgggctgctg gttgatgacc ctgcacatag cagggggttg gatctggatg agcactgtgc
     1441 tcctttgcaa cccaggccgt tctatgattc tgtcattcta aatctctctt tcagcctaaa
     1501 gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag
     1561 aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc
     1621 ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct
     1681 agcgggggag ggacgtaatt acatccctgg gggctttggg ggggggctgt ccctctagcg
     1741 agctcggtac ccagagcccc atcctcactg actccgtcct ggagttggat gagagataat
     1801 ggccttacgt tgtgccaggg gagggtcggg ctggatttag caagatttac cttctccaaa
     1861 gagcggtgct gcagtggcac agctgcccac ggaggtgggg gggtcaccgt ccctggaggt
     1921 gatgaagaac tgtggggatg tggcactgag ggacatggcc agtgggcacg gtgggtgggt
     1981 tggggttggt cttggggatc ttggagggct tttccagcct tcatgatttg acgattgtat
     2041 gaacatctac atggcaattc tccagctgcc tgtcccagtc ctactgaccc agctgtatct
     2101 ctccaggcaa gctcttccac cccttctgct tgcatccaga caccatcaaa catgcaggct
     2161 cagacacagg gaccagcagt gtctgtggcc tttttgtgct cctctccatg ctgggtttta
     2221 acttgctctt tgtccttcta tcctatcttc ttatccttaa ggctgttctg aacgctgtga
     2281 cttggagagt gtcccagagc cctcaacacc tgcatgtccc acgtccatgc tgtcctgcac
     2341 ttccttatcc ccaagatctg cctctccgtg atgcactgaa ttggcaaaca tgtgtcaccc
     2401 cagaccaaca atgtcacagc aaactccccc ttgataggac aagggggaat ggctttacac
     2461 tgagacaggg gaggtttggg ttggatatga ggaggcagtt tttcccccag agggtggtga
     2521 cgcactgaac aggttgccca aggaggctgt ggatgcccca tccctgcagg cattcaaggc
     2581 caggctggat gtggctctgg gcagcctggg ctgctggttg atgaccctgc acatagcagg
     2641 gggttggatc tggatgagca ctgtgctcct ttgcaaccca ggccgttcta tgattctgtc
     2701 attctaaatc tctctttcag cctaaagctt tttccccgta tccccccagg tgtctgcagg
     2761 ctcaaagagc agcgagaagc gttcagagga aagcgatccc gtgccacctt ccccgtgccc
     2821 gggctgtccc cgcacgctgc cggctcgggg atgcgggggg agcgccggac cggagcggag
     2881 ccccgggcgg ctcgctgctg ccccctagcg ggggagggac gtaattacat ccctgggggc
     2941 tttggggggg ggctgtccct ctaggggtac ccggggatcc accggtatcg attacctatc
     3001 acaaattcga gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc
     3061 agtgatagag aacgatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt
     3121 tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
     3181 gtatgtcgag tttatcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag
     3241 tgatagagaa cgtatgtcga ggtaggcgtg tacggtggga ggcctatata agcagagctc
     3301 gtttagtgaa ccgtcagatc gcctatttaa atcacgcgtg tttgggacgc gtgctagccg
     3361 ccaccatggt gagcaagggc gaggagctga tcaaggagaa catgcacatg aagctgtaca
     3421 tggaaggcac cgtgaacaac caccacttca agtgcaccac cgaaggggag ggcaagccct
     3481 acgagggcac ccagacccag aggattaagg tggtggaggg aggccccctg ccgttcgcat
     3541 tcgacatcct ggccacctgc tttatgtacg ggagcaagac cttcatcaac cacacccagg
     3601 gcatccccga tttctttaag cagtccttcc ctgagggctt cacatgggag agagtcacca
     3661 catacgaaga cgggggcgtg cttaccgtta cccaggacac cagcctccag gacggctgct
     3721 tgatctacaa cgtcaagctc agaggggtga acttcccatc caacggccct gtgatgcaga
     3781 agaaaacact cggctgggag gccaccaccg agaccctgta ccccgctgac ggcggcctgg
     3841 aaggcagatg cgacatggcc ctgaagctcg tgggcggggg ccacctgcac tgcaacctga
     3901 agaccacata cagatccaag aaacccgcta agaacctcaa gatgcccggc gtctactttg
     3961 tggaccgcag actggaaaga atcaaggagg ccgacaatga gacctacgtc gagcagcacg
     4021 aggtggctgt ggccagatac tgcgacctcc ctagcaaact ggggcacaaa cttaatggca
     4081 tggacgagct gtacaaggga agcggagcta ctaacttcag cctgctgaag caggctggag
     4141 acgtggagga gaaccctgga cctatgctcg atcctggaga ggtctatgat gaccctattg
     4201 acccaatcga gttagaggct gaacccagag gaacccccat tgtccccaac atcttgagga
     4261 actctgacta caatctcaac tctcctttga tagaagatcc tgctagacta atgttagaat
     4321 ggttaaaaac agggaataga ccttatcgga tgactctaac agacaattgc tccaggtctt
     4381 tcagagtttt gaaagattat ttcaagaagg tagatttggg ttctctcaag gtgggcggaa
     4441 tggctgcaca gtcaatgatt tctctctggt tatatggtgc ccactctgaa tccaacagga
     4501 gccggagatg tataacagac ttggcccatt tctattccaa gtcgtccccc atagagaagc
     4561 tgttgaatct cacgctagga aatagagggc tgagaatccc cccagaggga gtgttaagtt
     4621 gccttgagag ggttgattat gataatgcat ttggaaggta tcttgccaac acgtattcct
     4681 cttacttgtt cttccatgta atcaccttat acatgaacgc cctagactgg gatgaagaaa
     4741 agaccatcct agcattatgg aaagatttaa cctcagtgga catcgggaag gacttggtaa
     4801 agttcaaaga ccaaatatgg ggactgctga tcgtgacaaa ggactttgtt tactcccaaa
     4861 gttccaattg tctttttgac agaaactaca cacttatgct aaaagatctt ttcttgtctc
     4921 gcttcaactc cttaatggtc ttgctctctc ccccagagcc ccgatactca gatgacttga
     4981 tatctcaact atgccagctg tacattgctg gggatcaagt cttgtctatg tgtggaaact
     5041 ccggctatga agtcatcaaa atattggagc catatgtcgt gaatagttta gtccagagag
     5101 cagaaaagtt taggcctctc attcattcct tgggagactt tcctgtattt ataaaagaca
     5161 aggtaagtca acttgaagag acgttcggtc cctgtgcaag aaggttcttt agggctctgg
     5221 atcaattcga caacatacat gacttggttt ttgtgtttgg ctgttacagg cattgggggc
     5281 acccatatat agattatcga aagggtctgt caaaactata tgatcaggtt caccttaaaa
     5341 aaatgataga taagtcctac caggagtgct tagcaagcga cctagccagg aggatcctta
     5401 gatggggttt tgataagtac tccaagtggt atctggattc aagattccta gcccgagacc
     5461 accccttgac tccttatatc aaaacccaaa catggccacc caaacatatt gtagacttgg
     5521 tgggggatac atggcacaag ctcccgatca cgcagatctt tgagattcct gaatcaatgg
     5581 atccgtcaga aatattggat gacaaatcac attctttcac cagaacgaga ctagcttctt
     5641 ggctgtcaga aaaccgaggg gggcctgttc ctagcgaaaa agttattatc acggccctgt
     5701 ctaagccgcc tgtcaatccc cgagagtttc tgaggtctat agacctcgga ggattgccag
     5761 atgaagactt gataattggc ctcaagccaa aggaacggga attgaagatt gaaggtcgat
     5821 tctttgctct aatgtcatgg aatctaagat tgtattttgt catcactgaa aaactcttgg
     5881 ccaactacat cttgccactt tttgacgcgc tgactatgac agacaacctg aacaaggtgt
     5941 ttaaaaagct gatcgacagg gtcaccgggc aagggctttt ggactattca agggtcacat
     6001 atgcatttca cctggactat gaaaagtgga acaaccatca aagattagag tcaacagagg
     6061 atgtattttc tgtcctagat caagtgtttg gattgaagag agtgttttct agaacacacg
     6121 agttttttca aaaggcctgg atctattatt cagacagatc agacctcatc gggttacggg
     6181 aggatcaaat atactgctta gatgcgtcca acggcccaac ctgttggaat ggccaggatg
     6241 gcgggctaga aggcttacgg cagaagggct ggagtctagt cagcttattg atgatagata
     6301 gagaatctca aatcaggaac acaagaacca aaatactagc tcaaggagac aaccaggttt
     6361 tatgtccgac atacatgttg tcgccagggc tatctcaaga ggggctcctc tatgaattgg
     6421 agagaatatc aaggaatgca ctttcgatat acagagccgt cgaggaaggg gcatctaagc
     6481 tagggctgat catcaagaaa gaagagacca tgtgtagtta tgacttcctc atctatggaa
     6541 aaaccccttt gtttagaggt aacatattgg tgcctgagtc caaaagatgg gccagagtct
     6601 cttgcgtctc taatgaccaa atagtcaacc tcgccaatat aatgtcgaca gtgtccacca
     6661 atgcgctaac agtggcacaa cactctcaat ctttgatcaa accgatgagg gattttctgc
     6721 tcatgtcagt acaggcagtc tttcactacc tgctatttag cccaatctta aagggaagag
     6781 tttacaagat tctgagcgct gaaggggaga gctttctcct agccatgtca aggataatct
     6841 atctagatcc ttctttggga gggatatctg gaatgtccct cggaagattc catatacgac
     6901 agttctcaga ccctgtctct gaagggttat ccttctggag agagatctgg ttaagctccc
     6961 aagagtcctg gattcacgcg ttgtgtcaag aggctggaaa cccagatctt ggagagagaa
     7021 cactcgagag cttcactcgc cttctagaag atccgaccac cttaaatatc agaggagggg
     7081 ccagtcctac cattctactc aaggatgcaa tcagaaaggc tttatatgac gaggtggaca
     7141 aggtggaaaa ttcagagttt cgagaggcaa tcctgttgtc caagacccat agagataatt
     7201 ttatactctt cttaatatct gttgagcctc tgtttcctcg atttctcagt gagctattca
     7261 gttcgtcttt tttgggaatc cccgagtcaa tcattggatt gatacaaaac tcccgaacga
     7321 taagaaggca gtttagaaag agtctctcaa aaactttaga agaatccttc tacaactcag
     7381 agatccacgg gattagtcgg atgacccaga cacctcagag ggttgggggg gtgtggcctt
     7441 gctcttcaga gagggcagat ctacttaggg agatctcttg gggaagaaaa gtggtaggca
     7501 cgacagttcc tcacccttct gagatgttgg gattacttcc caagtcctct atttcttgca
     7561 cttgtggagc aacaggagga ggcaatccta gagtttctgt atcagtactc ccgtcctttg
     7621 atcagtcatt tttttcacga ggccccctaa agggatactt gggctcgtcc acctctatgt
     7681 cgacccagct attccatgca tgggaaaaag tcactaatgt tcatgtggtg aagagagctc
     7741 tatcgttaaa agaatctata aactggttca ttactagaga ttccaacttg gctcaagctc
     7801 taattaggaa cattatgtct ctgacaggcc ctgatttccc tctagaggag gcccctgtct
     7861 tcaaaaggac ggggtcagcc ttgcataggt tcaagtctgc cagatacagc gaaggagggt
     7921 attcttctgt ctgcccgaac ctcctctctc atatttctgt tagtacagac accatgtctg
     7981 atttgaccca agacgggaag aactacgatt tcatgttcca gccattgatg ctttatgcac
     8041 agacatggac atcagagctg gtacagagag acacaaggct aagagactct acgtttcatt
     8101 ggcacctccg atgcaacagg tgtgtgagac ccattgacga cgtgaccctg gagacctctc
     8161 agatcttcga gtttccggat gtgtcgaaaa gaatatccag aatggtttct ggggctgtgc
     8221 ctcacttcca gaggcttccc gatatccgtc tgagaccagg agattttgaa tctctaagcg
     8281 gtagagaaaa gtctcaccat atcggatcag ctcaggggct cttatactca atcttagtgg
     8341 caattcacga ctcaggatac aatgatggaa ccatcttccc tgtcaacata tacggcaagg
     8401 tttcccctag agactatttg agagggctcg caaggggagt attgatagga tcctcgattt
     8461 gcttcttgac aagaatgaca aatatcaata ttaatagacc tcttgaattg gtctcagggg
     8521 taatctcata tattctcctg aggctagata accatccctc cttgtacata atgctcagag
     8581 aaccgtctct tagaggagag atattttcta tccctcagaa aatccccgcc gcttatccaa
     8641 ccactatgaa agaaggcaac agatcaatct tgtgttatct ccaacatgtg ctacgctatg
     8701 agcgagagat aatcacggcg tctccagaga atgactggct atggatcttt tcagacttta
     8761 gaagtgccaa aatgacgtac ctatccctca ttacttacca gtctcatctt ctactccaga
     8821 gggttgagag aaacctatct aagagtatga gagataacct gcgacaattg agttctttga
     8881 tgaggcaggt gctgggcggg cacggagaag ataccttaga gtcagacgac aacattcaac
     8941 gactgctaaa agactcttta cgaaggacaa gatgggtgga tcaagaggtg cgccatgcag
     9001 ctagaaccat gactggagat tacagcccca acaagaaggt gtcccgtaag gtaggatgtt
     9061 cagaatgggt ctgctctgct caacaggttg cagtctctac ctcagcaaac ccggcccctg
     9121 tctcggagct tgacataagg gccctctcta agaggttcca gaaccctttg atctcgggct
     9181 tgagagtggt tcagtgggca accggtgctc attataagct taagcctatt ctagatgatc
     9241 tcaatgtttt cccatctctc tgccttgtag ttggggacgg gtcagggggg atatcaaggg
     9301 cagtcctcaa catgtttcca gatgccaagc ttgtgttcaa cagtctttta gaggtgaatg
     9361 acctgatggc ttccggaaca catccactgc ctccttcagc aatcatgagg ggaggaaatg
     9421 atatcgtctc cagagtgata gatcttgact caatctggga aaaaccgtcc gacttgagaa
     9481 acttggcaac ctggaaatac ttccagtcag tccaaaagca ggtcaacatg tcctatgacc
     9541 tcattatttg cgatgcagaa gttactgaca ttgcatctat caaccggatc accctgttaa
     9601 tgtccgattt tgcattgtct atagatggac cactctattt ggtcttcaaa acttatggga
     9661 ctatgctagt aaatccaaac tacaaggcta ttcaacacct gtcaagagcg ttcccctcgg
     9721 tcacagggtt tatcacccaa gtaacttcgt ctttttcatc tgagctctac ctccgattct
     9781 ccaaacgagg gaagtttttc agagatgctg agtacttgac ctcttccacc cttcgagaaa
     9841 tgagccttgt gttattcaat tgtagcagcc ccaagagtga gatgcagaga gctcgttcct
     9901 tgaactatca ggatcttgtg agaggatttc ctgaagaaat catatcaaat ccttacaatg
     9961 agatgatcat aactctgatt gacagtgatg tagaatcttt tctagtccac aagatggttg
    10021 atgatcttga gttacagagg ggaactctgt ctaaagtggc tatcattata gccatcatga
    10081 tagttttctc caacagagtc ttcaacgttt ccaaacccct aactgacccc tcgttctatc
    10141 caccgtctga tcccaaaatc ctgaggcact tcaacatatg ttgcagtact atgatgtatc
    10201 tatctactgc tttaggtgac gtccctagct tcgcaagact tcacgacctg tataacagac
    10261 ctataactta ttacttcaga aagcaagtca ttcgagggaa cgtttatcta tcttggagtt
    10321 ggtccaacga cacctcagtg ttcaaaaggg tagcctgtaa ttctagcctg agtctgtcat
    10381 ctcactggat caggttgatt tacaagatag tgaagactac cagactcgtt ggcagcatca
    10441 aggatctatc cagagaagtg gaaagacacc ttcataggta caacaggtgg atcaccctag
    10501 aggatatcag atctagatca tccctactag actacagttg cctgtgaacg cgttacgtaa
    10561 agcttgtcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
    10621 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
    10681 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
    10741 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
    10801 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
    10861 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
    10921 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
    10981 cttggctgct cgcctatgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
    11041 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
    11101 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
    11161 atcgacaccg agcgctgctc gaggcctcga ctgtgccttc tagttgccag ccatctgttg
    11221 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
    11281 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
    11341 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggaca
    11401 cgtgggatcc tctagagccc catcctcact gactccgtcc tggagttgga tgagagataa
    11461 tggccttacg ttgtgccagg ggagggtcgg gctggattta gcaagattta ccttctccaa
    11521 agagcggtgc tgcagtggca cagctgccca cggaggtggg ggggtcaccg tccctggagg
    11581 tgatgaagaa ctgtggggat gtggcactga gggacatggc cagtgggcac ggtgggtggg
    11641 ttggggttgg tcttggggat cttggagggc ttttccagcc ttcatgattt gacgattgta
    11701 tgaacatcta catggcaatt ctccagctgc ctgtcccagt cctactgacc cagctgtatc
    11761 tctccaggca agctcttcca ccccttctgc ttgcatccag acaccatcaa acatgcaggc
    11821 tcagacacag ggaccagcag tgtctgtggc ctttttgtgc tcctctccat gctgggtttt
    11881 aacttgctct ttgtccttct atcctatctt cttatcctta aggctgttct gaacgctgtg
    11941 acttggagag tgtcccagag ccctcaacac ctgcatgtcc cacgtccatg ctgtcctgca
    12001 cttccttatc cccaagatct gcctctccgt gatgcactga attggcaaac atgtgtcacc
    12061 ccagaccaac aatgtcacag caaactcccc cttgatagga caagggggaa tggctttaca
    12121 ctgagacagg ggaggtttgg gttggatatg aggaggcagt ttttccccca gagggtggtg
    12181 acgcactgaa caggttgccc aaggaggctg tggatgcccc atccctgcag gcattcaagg
    12241 ccaggctgga tgtggctctg ggcagcctgg gctgctggtt gatgaccctg cacatagcag
    12301 ggggttggat ctggatgagc actgtgctcc tttgcaaccc aggccgttct atgattctgt
    12361 cattctaaat ctctctttca gcctaaagct ttttccccgt atccccccag gtgtctgcag
    12421 gctcaaagag cagcgagaag cgttcagagg aaagcgatcc cgtgccacct tccccgtgcc
    12481 cgggctgtcc ccgcacgctg ccggctcggg gatgcggggg gagcgccgga ccggagcgga
    12541 gccccgggcg gctcgctgct gccccctagc gggggaggga cgtaattaca tccctggggg
    12601 ctttgggggg gggctgtccc tctagagccc catcctcact gactccgtcc tggagttgga
    12661 tgagagataa tggccttacg ttgtgccagg ggagggtcgg gctggattta gcaagattta
    12721 ccttctccaa agagcggtgc tgcagtggca cagctgccca cggaggtggg ggggtcaccg
    12781 tccctggagg tgatgaagaa ctgtggggat gtggcactga gggacatggc cagtgggcac
    12841 ggtgggtggg ttggggttgg tcttggggat cttggagggc ttttccagcc ttcatgattt
    12901 gacgattgta tgaacatcta catggcaatt ctccagctgc ctgtcccagt cctactgacc
    12961 cagctgtatc tctccaggca agctcttcca ccccttctgc ttgcatccag acaccatcaa
    13021 acatgcaggc tcagacacag ggaccagcag tgtctgtggc ctttttgtgc tcctctccat
    13081 gctgggtttt aacttgctct ttgtccttct atcctatctt cttatcctta aggctgttct
    13141 gaacgctgtg acttggagag tgtcccagag ccctcaacac ctgcatgtcc cacgtccatg
    13201 ctgtcctgca cttccttatc cccaagatct gcctctccgt gatgcactga attggcaaac
    13261 atgtgtcacc ccagaccaac aatgtcacag caaactcccc cttgatagga caagggggaa
    13321 tggctttaca ctgagacagg ggaggtttgg gttggatatg aggaggcagt ttttccccca
    13381 gagggtggtg acgcactgaa caggttgccc aaggaggctg tggatgcccc atccctgcag
    13441 gcattcaagg ccaggctgga tgtggctctg ggcagcctgg gctgctggtt gatgaccctg
    13501 cacatagcag ggggttggat ctggatgagc actgtgctcc tttgcaaccc aggccgttct
    13561 atgattctgt cattctaaat ctctctttca gcctaaagct ttttccccgt atccccccag
    13621 gtgtctgcag gctcaaagag cagcgagaag cgttcagagg aaagcgatcc cgtgccacct
    13681 tccccgtgcc cgggctgtcc ccgcacgctg ccggctcggg gatgcggggg gagcgccgga
    13741 ccggagcgga gccccgggcg gctcgctgct gccccctagc gggggaggga cgtaattaca
    13801 tccctggggg ctttgggggg gggctgtccc tctagagtcg acggcgcgcc gtaaaagctt
    13861 ggcatagatc ttattgcaga agccatatgt tttaggtgcc agggcgtgcc cttgggctcc
    13921 ccgggcgcgt ttgggcggcc gcatcgaatt ctaccgggta ggggaggcgc ttttcccaag
    13981 gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct
    14041 ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg
    14101 ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct
    14161 cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga
    14221 cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg
    14281 ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg
    14341 cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct
    14401 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg
    14461 caatcgccgc tagccgccac catgaaaaag cctgaactca ccgcgacgtc tgtcgagaag
    14521 tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa
    14581 tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc
    14641 gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg
    14701 attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc
    14761 cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag
    14821 ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc
    14881 ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg
    14941 attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgtaagt
    15001 gctagcccct aggcctgaat cgaagttcct attcttcaaa aggtatagga acttcgagct
    15061 cgtcgactgc agaggcctgc atgcaagctt ggcgtaatca tggtcatagc tgtttcctgt
    15121 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
    15181 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
    15241 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
    15301 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
    15361 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
    15421 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
    15481 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
    15541 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
    15601 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
    15661 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
    15721 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
    15781 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    15841 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
    15901 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
    15961 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    16021 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    16081 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
    16141 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
    16201 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
    16261 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
    16321 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
    16381 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    16441 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    16501 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    16561 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    16621 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    16681 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    16741 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    16801 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    16861 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    16921 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    16981 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    17041 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    17101 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    17161 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    17221 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
    17281 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc
//
