LOCUS       sequence-352009-       10424 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains codon-optimized Sth1Cas9 and gRNA for streptomyces.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10424)
  AUTHORS   Yeo WL, Heng E, Tan LL, Lim YW, Lim YH, Hoon S, Zhao H, Zhang MM, 
            Wong FT
  TITLE     Characterization of Cas proteins for CRISPR-Cas editing in 
            streptomycetes.
  JOURNAL   Biotechnol Bioeng. 2019 May 15. doi: 10.1002/bit.27021.
  PUBMED    31090220
REFERENCE   2  (bases 1 to 10424)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 May 15. doi: 10.1002/bit.27021."
COMMENT     Sequence Label: pCRISPomyces-Sth1Cas9
FEATURES             Location/Qualifiers
     source          1..10424
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..302
                     /label=rpsL promoter
                     /note="promoter for the Xylanimonas cellulosilytica 
                     ribosomal protein S12 gene"
     promoter        3868..4152
                     /label=gapdh promoter
                     /note="glyceraldehyde-3-phosphate dehydrogenase promoter 
                     from Eggerthella lenta"
     primer_bind     4307..4329
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4321..4338
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4322..4338
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4348..4367
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4348..4366
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4414..4434)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4414..4432)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4453..4469)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4453..4469)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4466..4488)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4477..4493
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4501..4507,4508..4525,4526..4531))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4546..4567
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      4706..4740
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     rep_origin      complement(4954..5542)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5034..5053)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             5746..6546
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     CDS             complement(7485..8927)
                     /codon_start=1
                     /gene="rep"
                     /product="replication initiator protein from the 
                     Streptomyces ghanaensis plasmid pSG5"
                     /label=pSG5 Rep
                     /translation="MWKPGEATWGNTCRCNNVHTCPWCMSRILAVRGSNVQLAADGLAD
                     AGYGLHLGTNTLRHFERMAFGTVRKGMRHGLVAVLHDGWKGAYGSSGRRWRTMRDDFGI
                     IGYERAFEDTFGWGSGWHLHWHTLWVTREVLGPDAQAAFRDALAGAWAAGVESAGGYTV
                     SETCDRPGCSCEGKGHGTDVRPLNGADAADGDAGKQARYLYKDGDKTKGGVAKIGLELA
                     GQNFKAGRGDDRMGPLDLGDAAAAELQRLRRPGPFVEKYREREFGVFQVRKHYRSQNLN
                     RLIKELGIQQDVRTEEEITDDTEGLVAIAVIPAYIWYRYIARVAGRRLDLIKVAETYGL
                     PGVRRLVESWGLVWGKDVLDPPAPEAPAAPGDLDADQMRFEVMSEEEAAFREARRKANE
                     ARTEELAASLDRVRQPKKEAIRPTISLRKRLKPKPVTVDVKTPPPGAASPVCRRCKGKL
                     APVLQPWGQHPGDCLRVDTAVA"
     oriT            10094..10203
                     /note="incP origin of transfer"
ORIGIN
        1 gccctgcagg cggaagtcag gtagacacga cttccgctag tccttgcaag gtctgctgac
       61 gtgaggcggg gcggtcgttt ttgaccgccc tgccttcgtc atgtaggctc gctcgctgtg
      121 cctggcgtgt catcagacgc ccaggtcccg gtgccgtgag gcccgggcca tcgagccggt
      181 ggtacgtggc tgcggtcccc ttgtgagggc tgcgcgccgt gtgctgtccg gcgcgcacag
      241 ccttgaatcc acccgcgggg gccggccggt ctccgtgagc tcgagtagac gacggagacg
      301 taatgtcgga cctggtgctg ggcctggaca tcggcatcgg ctccgtcggc gtcggcatcc
      361 tgaacaaggt gaccggcgag atcatccaca agaactcccg catcttcccg gccgcacagg
      421 ccgagaacaa cctggtccgc cgcaccaacc gccagggccg ccgcctggcc cgccgcaaga
      481 agcaccgccg cgtccgcctg aaccgcctgt tcgaggagtc cggcctgatc accgacttca
      541 ccaagatcag catcaacctg aacccgtacc agctgcgcgt caagggcctg accgacgagc
      601 tgtccaacga ggagctgttc atcgccctga agaacatggt caagcaccgc ggcatctcct
      661 acctggacga cgcctccgac gacggcaact cctccgtcgg cgactacgcc cagatcgtca
      721 aggagaactc caagcagctg gaaaccaaga cccccggcca gatccagctg gagcgctacc
      781 agacctacgg ccagctgcgc ggcgacttca ccgtcgagaa ggacggcaag aagcaccgcc
      841 tgatcaacgt gttcccgacc tccgcctacc gctccgaggc cctgcgcatc ctccagaccc
      901 agcaggagtt caacccgcag atcaccgacg agttcatcaa ccgctacctg gagatcctga
      961 ccggcaagcg caagtactac cacggccccg gcaacgagaa gtcccgcacc gactacggcc
     1021 gctaccgcac ctccggcgag acgctggaca acatcttcgg catcctgatc ggcaagtgca
     1081 ccttctaccc ggacgagttc cgcgccgcca aggcctccta caccgcccag gagttcaacc
     1141 tgctgaacga cctgaacaac ctgaccgtcc cgaccgagac gaagaagctg tccaaggagc
     1201 agaagaacca gatcatcaac tacgtcaaga acgagaaggc gatgggcccg gccaagctgt
     1261 tcaagtacat cgccaagctg ctgtcctgcg acgtcgccga catcaagggc taccgcatcg
     1321 acaagtccgg caaggccgag atccacacct tcgaggccta ccgcaagatg aaaaccctgg
     1381 agacgctgga catcgagcag atggaccgcg agacgctgga caagctggcc tacgtcctga
     1441 ccctgaacac cgagcgggag ggcatccagg aagccctgga gcacgagttc gccgacggct
     1501 ccttctccca gaagcaggtg gacgagctgg tccagttccg caaggccaac tcctccatct
     1561 tcggcaaggg ctggcacaac ttctccgtca agctgatgat ggagctgatc ccggagctgt
     1621 acgagacgtc cgaggagcag atgaccatcc tgacccgcct gggcaagcaa aagaccacct
     1681 cctcctccaa caagaccaag tacatcgacg agaagctgct gaccgaggaa atctacaacc
     1741 cggtcgtcgc caagtccgtc cgccaggcca tcaagatcgt caacgccgcc atcaaggagt
     1801 acggcgactt cgacaacatc gtcatcgaga tggcccgcga gacgaacgag gacgacgaga
     1861 agaaggccat ccagaagatc cagaaggcca acaaggacga gaaggacgcc gccatgctga
     1921 aggccgccaa ccagtacaac ggcaaggccg agctgccgca ctccgtgttc cacggccaca
     1981 agcagctggc caccaagatc cggctgtggc accagcaggg cgagcgctgc ctgtacaccg
     2041 gcaagaccat ctccatccac gacctgatca acaactccaa ccagttcgag gtggaccaca
     2101 tcctgccgct gtccatcacc ttcgacgact ccctggccaa caaggtcctg gtctacgcca
     2161 ccgccaacca ggagaagggc cagcgcaccc cgtaccaggc cctggactcg atggacgacg
     2221 cctggtcctt ccgcgagctg aaggccttcg tccgcgagtc caagaccctg tccaacaaga
     2281 agaaggagta cctgctgacc gaagaggaca tctccaagtt cgacgtccgc aagaagttca
     2341 tcgagcgcaa cctggtggac acccggtacg cctcccgcgt cgtcctgaac gccctccagg
     2401 agcacttccg cgcccacaag atcgacacca aggtgtccgt cgtccgcggc cagttcacct
     2461 cccagctccg ccgccactgg ggcatcgaaa agacccgcga cacctaccac caccacgccg
     2521 tggacgccct gatcatcgcc gcctcctccc agctgaacct gtggaagaag cagaagaaca
     2581 ccctggtgtc ctactccgag gaccagctgc tggacatcga gacgggcgag ctgatctccg
     2641 acgacgagta caaggagtcc gtgttcaagg ccccgtacca gcacttcgtg gacaccctga
     2701 agtccaagga gttcgaggac tccatcctgt tctcctacca ggtggactcc aagttcaacc
     2761 gcaagatcag cgacgccacc atctacgcca cccgccaggc caaggtcggc aaggacaagg
     2821 ccgacgagac gtacgtcctg ggcaagatca aggacatcta cacccaggac ggctacgacg
     2881 ccttcatgaa aatctacaag aaggacaagt ccaagttcct gatgtaccgc cacgacccgc
     2941 agaccttcga gaaggtcatc gagccgatcc tggagaacta cccgaacaag cagatcaacg
     3001 acaagggcaa ggaagtcccg tgcaacccgt tcctgaagta caaggaagag cacggctaca
     3061 tccgcaagta ctccaagaag ggcaacggcc cggagatcaa gtccctgaag tactacgact
     3121 ccaagctggg caaccacatc gacatcaccc cgaaggactc caacaacaag gtcgtcctcc
     3181 agtccgtctc cccgtggcgc gccgacgtct acttcaacaa gaccaccggc aagtacgaga
     3241 tcctgggcct gaagtacgcc gacctccagt tcgacaaggg caccggcacc tacaagatca
     3301 gccaggagaa gtacaacgac atcaagaaga aggaaggcgt ggactccgac tccgagttca
     3361 agttcaccct gtacaagaac gacctgctgc tggtcaagga caccgagacg aaggagcagc
     3421 agctgttccg cttcctgtcc cgcaccatgc cgaagcagaa gcactacgtc gagctgaagc
     3481 cgtacgacaa gcagaagttc gagggcggcg aggccctgat caaggtcctg ggcaacgtcg
     3541 ccaactccgg ccagtgcaag aagggcctgg gcaagtccaa catctccatc tacaaggtcc
     3601 gcaccgacgt cctgggcaac cagcacatca tcaagaacga gggcgacaag ccgaagctcg
     3661 acttctgaga attcagatct acgcgttccc cgcaaaagcg gcctttgact ccctgcaagc
     3721 ctcagcgacc gaatatatcg gttatgcgtg ggcgatggtt gttgtcattg tcggcgcaac
     3781 tatcggtatc aagctgttta agaaattcac ctcgaaagca agctgataaa ccgatacaat
     3841 taaaggctcc ttttggagcc tttttttgct gctccttcgg tcggacgtgc gtctacgggc
     3901 accttaccgc agccgtcggc tgtgcgacac ggacggatcg ggcgaactgg ccgatgctgg
     3961 gagaagcgcg ctgctgtacg gcgcgcaccg ggtgcggagc ccctcggcga gcggtgtgaa
     4021 acttctgtga atggcctgtt cggttgcttt ttttatacgg ctgccagata aggcttgcag
     4081 catctgggcg gctaccgcta tgatcggggc gttcctgcaa ttcttagtgc gagtatctga
     4141 aaggggatac gcaagtcttc tcagccgcta cagggcgcgt cccattcgcc attcaggctg
     4201 cgcaactgtt gggaaggggg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     4261 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     4321 tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga attgggtacc
     4381 gggccccccc tcgaggtcct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
     4441 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     4501 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     4561 cacattaatt gcgttgcgct cactgcccgc tttccaccgg tgaagacatg tttttgtact
     4621 cgaaagaagc tacaaagata aggcttcatg ccgaaatcaa caccctgtca ttttatggca
     4681 gggtgtttta ctccatctgg atttgttcag aacgctcggt tgccgccggg cgttttttat
     4741 ctagagctag catgcatatg aagctttgtt cacattcgaa cggtctctgc tttgacaaca
     4801 tgctgtgcgg tgttgtaaag tcgtggccag gagaatacga cagcgtgcag gactggggga
     4861 gttatgacta gtgtgaaaag tcagtatcca gtcgtgtagt tcttattacc ttctagaggc
     4921 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     4981 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     5041 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     5101 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     5161 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgacccccc
     5221 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     5281 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     5341 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     5401 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     5461 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     5521 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     5581 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     5641 ctagatcctt ttggttcatg tgcagctcca tcagcaaaag gggatgataa gtttatcacc
     5701 accgactatt tgcaacagtg ccgttgatcg tgctatgatc gactgatgtc atcagcggtg
     5761 gagtgcaatg tcgtgcaata cgaatggcga aaagccgagc tcatcggtca gcttctcaac
     5821 cttggggtta cccccggcgg tgtgctgctg gtccacagct ccttccgtag cgtccggccc
     5881 ctcgaagatg ggccacttgg actgatcgag gccctgcgtg ctgcgctggg tccgggaggg
     5941 acgctcgtca tgccctcgtg gtcaggtctg gacgacgagc cgttcgatcc tgccacgtcg
     6001 cccgttacac cggaccttgg agttgtctct gacacattct ggcgcctgcc aaatgtaaag
     6061 cgcagcgccc atccatttgc ctttgcggca gcggggccac aggcagagca gatcatctct
     6121 gatccattgc ccctgccacc tcactcgcct gcaagcccgg tcgcccgtgt ccatgaactc
     6181 gatgggcagg tacttctcct cggcgtggga cacgatgcca acacgacgct gcatcttgcc
     6241 gagttgatgg caaaggttcc ctatggggtg ccgagacact gcaccattct tcaggatggc
     6301 aagttggtac gcgtcgatta tctcgagaat gaccactgct gtgagcgctt tgccttggcg
     6361 gacaggtggc tcaaggagaa gagccttcag aaggaaggtc cagtcggtca tgcctttgct
     6421 cggttgatcc gctcccgcga cattgtggcg acagccctgg gtcaactggg ccgagatccg
     6481 ttgatcttcc tgcatccgcc agaggcggga tgcgaagaat gcgatgccgc tcgccagtcg
     6541 attggctgag ctcatgagcg gagaacgaga tgacgttgga ggggcaaggt cgcgctgatt
     6601 gctggggcaa cacgtggagc ggatcgggga ttgtctttct tcagctcgct gatgatatgc
     6661 tgacgctcaa tgccgtttgg cctccgacta acgaaaatcc cgcatttgga cggctgatcc
     6721 gattggcacg gcggacggcg aatggcggag cagacgctcg tccgggggca atgagatatg
     6781 aaaaagcctg aactcaccgc gacgtatcga tgtcgaggtt cctcagggga gccaccccag
     6841 agaagccctc ggagctgagc ggagctattt ccaaagccat gccagctaga gacagtgcac
     6901 accgccaagc atctgcaaaa ccctcgcctg gagggaaagt gcaatgtacg tacgctggtt
     6961 tccctccaga aagggattct gcggcttctc aactttggaa gaagaggcgg aacgaactgc
     7021 tgtttcagcc tactcatgtg aggaggctgg tcctttaccc tgatgcccga gggcatcagg
     7081 gtaaaggacc agcctcgcca actaggagcg ctaccaaggc gaacacaacc caggacttgg
     7141 tagaaccctt cgccggagaa gttcagactc atcggcatga gcacccctcg ccgcgcactc
     7201 ggcaccggtc cgtcgaccac caccaggacg tcgttgtcga cgtcggcccc gcggctcctg
     7261 cccgccgaac gcgtcgtcgt cgacggcctg gtgctcatcg acgagcaccc ggagccaggt
     7321 gaaaagcgcc ggcggacgct cggactgggc gcgggattcc agcagtaacc caggtccgcc
     7381 ggccacctca cggcaggcag accctcggct ttcgcgccgg gaccgccatg agaccgccac
     7441 ccggatgtcc ggggtggcgg tctcatggcg gtccctcagc ggccctacgc gaccgctgtg
     7501 tcgacgcgga ggcagtctcc ggggtgctgg ccccagggct ggagtaccgg ggcgagcttg
     7561 cccttgcagc ggcggcacac gggcgacgcg gcgccgggcg ggggagtctt gacgtcgacg
     7621 gtgaccggct tcggcttcag gcgcttccga aggctgatcg tcgggcggat cgcctccttc
     7681 ttcggctgac gcactcggtc gagcgaagcg gccagctcct ccgtgcgggc ctcgttggcc
     7741 ttccggcgcg cctcgcggaa agcagcttcc tcctcggaca tgacctcgaa cctcatctgg
     7801 tcagcgtcaa ggtcgcccgg cgcggccggc gcttccgggg cgggcgggtc caggacgtcc
     7861 ttgccccaca ccaggcccca ggactcgacg agccgccgga cgcccggtag gccgtacgtc
     7921 tcggcgacct tgatgaggtc gaggcgacgt ccggcgacgc gggcgatgta tcggtaccag
     7981 atgtaggccg ggatgaccgc gatggcgacc aggccctcgg tgtcgtcggt gatctcctcc
     8041 tcggtgcgga cgtcctgctg gatgccgagt tccttgatca gccggttcag gttctgcgac
     8101 cggtagtgct tgcggacctg gaacacgccg aactcgcgct cgcggtactt ctcgacgaac
     8161 gggccgggcc gacgaagccg ctgcagctcg gcggccgccg cgtcgcccag gtcgagcggt
     8221 cccatgcggt cgtcgccgcg accggccttg aagttctgtc cggccagctc caggccgatc
     8281 ttggcgacgc cgcccttggt cttgtcgccg tccttgtaga ggtagcgggc ctgcttgccc
     8341 gcatcgccgt cagcggcgtc cgcgccgttg agtgggcgca cgtcggtgcc gtggcccttg
     8401 ccctcacagg agcaaccggg ccggtcgcac gtctcgctga cggtgtagcc gcccgcggat
     8461 tcgaccccgg cggcccaggc tccggcgagt gcgtcgcgga acgcggcctg ggcgtccggg
     8521 ccgagcacct cgcgggtgac ccagagcgtg tgccagtgca ggtgccagcc ggagccccag
     8581 ccgaaggtgt cctcgaaggc ccgctcgtag ccgatgatcc cgaagtcgtc gcgcatcgtg
     8641 cgccagcggc ggccggacga gccgtacgcg cccttccagc cgtcgtgcaa gaccgcgacc
     8701 aggccgtgcc gcattccctt gcggacggtg ccgaacgcca tgcgctcgaa gtggcgcaac
     8761 gtgttcgtgc caaggtgcag cccgtacccg gcgtccgcga gaccgtcggc ggcgagctgc
     8821 acgttcgagc cccgtacggc caggatgcgg ctcatgcacc acgggcaggt gtggacgttg
     8881 ttgcagcggc acgtgttgcc ccacgtcgcc tcgcccggct tccacatcag ctcggccgtc
     8941 ccggcagtga gccgggtccc gcagcccttg aacgcctcgt tcagcgacac cgtctggtgc
     9001 cggtcccgcc gggcgaaccg ctcgtcgcgc gggtcctccc gcccggctgt cgcggcaccc
     9061 tcgtttgggg tagaacccgt tccagttaca gcgctctgac ctgcagtgga cggagatttt
     9121 cccttactac taaagcccgc gtccggatta cccgctgtag tcgtgcttgc tacgctgcgt
     9181 gactggtccg caatgagacg ctttgcgcgc tttcggcagg cgtccgagca gtagattttg
     9241 gggcgcttcc cggggatgtg gacgatcggg gtgccgcagt ggcacttcgg tccggcgggg
     9301 cgcggtggtg tcgacgcgct gttctctcgt acgctcgtca cagagcaaac gtcctcactc
     9361 ggcatgctgc gccggttcgg gggcggcgag cccgggaggc caatcccggg ctcgtgccat
     9421 ttctgggtcc tgttgatcct ggcattggtg tggccgttca ttgcccctgc tcgctcctga
     9481 cgcgccgata gacgtccgat acgcccggtg ctggtgggat ttgataggtc ggaagaagcc
     9541 ccgcccgggc ctgggcgggg cttcctgtgc gtcaggacct cctcgtcgtg agcctcttcg
     9601 gcctatggac ggagtgacct cgtgatccgt tacagccgcg cgcgctcgcg tagagcggtc
     9661 tcatcagttc cacgaacggt cctcttcgca gatcagggcg ttggggcgga gtctcaccaa
     9721 ggactacgtc tgctggcgat ttccgttaca ccccgggcgg tggccggcgc acacgcgcgc
     9781 ccgcgttggg cagtgcagaa agtgcagaaa cctaggcgct gatggtccag gtccacggtt
     9841 cgtcgtcggc ggcggcgcgg gcggcggcgt cggccagggc gcgggcgaga ccggctacgg
     9901 cgggcttgat gcgccggttg cgggcgacct tgagcagcta gtatgcaggt cgacggatct
     9961 tttccgctgc ataaccctgc ttcggggtca ttatagcgat tttttcggta tatccatcct
    10021 ttttcgcacg atatacagga ttttgccaaa gggttcgtgt agactttcct tggtgtatcc
    10081 aacggcgtca gccgggcagg ataggtgaag taggcccacc cgcgagcggg tgttccttct
    10141 tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc
    10201 cggctaccgc cggcgtaaca gatgagggca agcggatggc tgatgaaacc aagccaacca
    10261 ggaagggcag cccacctatc aaggtgtact gccttccaga cgaacgaaga gcgattgagg
    10321 aaaaggcggc ggcggccggc atgagcctgt cggcctacct gctggccgtc ggccagggct
    10381 acaaaatcac gggcgtcgtg gactatgagc acgtccgcga gctg
//
