LOCUS       sequence-352015-       12365 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Streptomyces codon optimized FnCas12a. Used to modify 
            genome of Streptomyces..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12365)
  AUTHORS   Zhang J, Zhang D, Zhu J, Liu H, Liang S, Luo Y
  TITLE     Efficient Multiplex Genome Editing in Streptomyces via Engineered 
            CRISPR-Cas12a Systems.
  JOURNAL   Front Bioeng Biotechnol. 2020 Jun 30;8:726. doi: 
            10.3389/fbioe.2020.00726. eCollection 2020.
  PUBMED    32695773
REFERENCE   2  (bases 1 to 12365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2020-06-30"; volume: "8:726. doi"; 
            pages: " 10.3389/fbioe.2020.00726. eCollection 2020"
COMMENT     Sequence Label: pYL-kasOp-FnCas12a2
FEATURES             Location/Qualifiers
     source          1..12365
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4212..4496
                     /label=gapdh promoter
                     /note="glyceraldehyde-3-phosphate dehydrogenase promoter 
                     from Eggerthella lenta"
     protein_bind    4537..4558
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4573..4578,4579..4596,4597..4603)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4611..4627
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4616..4638
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4635..4651
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4635..4651
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4647..4943
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSG"
     primer_bind     4670..4690
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4672..4690
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4737..4756)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4738..4756)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4766..4783)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4766..4782)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4775..4797)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      4968..5002
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     misc_feature    5034..5537
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     5578..5596
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5634..5656)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5756..5775
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5799..6014
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             6015..6818
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(6895..7483)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6975..6994)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             7687..8487
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     CDS             complement(9426..10868)
                     /codon_start=1
                     /gene="rep"
                     /product="replication initiator protein from the 
                     Streptomyces ghanaensis plasmid pSG5"
                     /label=pSG5 Rep
                     /translation="MWKPGEATWGNTCRCNNVHTCPWCMSRILAVRGSNVQLAADGLAD
                     AGYGLHLGTNTLRHFERMAFGTVRKGMRHGLVAVLHDGWKGAYGSSGRRWRTMRDDFGI
                     IGYERAFEDTFGWGSGWHLHWHTLWVTREVLGPDAQATFRDALAGAWAAGVESAGGYTV
                     SETCDRPGCSCEGKGHGTDVRPLNGADAADGDAGKQARYLYKDGDKTKGGVAKIGLELA
                     GQNFKAGRGDDRMGPLDLGDAAAAELQRLRRPGPFVEKYREREFGVFQVRKHYRSQNLN
                     RLIKELGIQQDVRTEEEITDDTEGLVAIAVIPAYIWYRYIARVAGRRLDLIKVAETYGL
                     PGVRRLVESWGLVWGKDVLDPPAPEAPAAPGDLDADQMRFEVMSEEEAAFREARRKANE
                     ARTEELAASLDRVRQPKKEAIRPTISLRKRLKPKPVTVDVKTPPPGAASPVCRRCKGKL
                     APVLQPWGQHPGDCLRVDTAVA"
     oriT            12035..12144
                     /note="incP origin of transfer"
ORIGIN
        1 aagctttgtt cacattcgaa cggtctctgc tttgacaaca tgctgtgcgg tgttgtaaag
       61 tcgtggccag gagaatacga cagcgtgcag gactggggga gttggatcca tgtccatcta
      121 ccaagaattt gtgaacaagt actcgctgtc caaaacgctg cgctttgaac tcatcccgca
      181 aggtaaaacg ctggagaaca tcaaagcgcg ggggctgatc ctcgacgatg aaaagcgcgc
      241 caaggactac aagaaggcca agcaaatcat cgacaagtac catcagttct ttatcgagga
      301 gattttatcc tccgtgtgca tttccgagga tttactccaa aactactccg acgtctattt
      361 taagctgaag aagtccgacg acgacaattt acaaaaggat ttcaagtccg cgaaggacac
      421 gatcaaaaaa cagatctcgg agtacattaa ggattccgag aagttcaaaa atttatttaa
      481 ccagaattta attgacgcca aaaaggggca agaatcggat ttaattctct ggctgaagca
      541 gtccaaggac aacgggatcg agctgttcaa ggccaattcc gacatcacgg atattgacga
      601 ggcgctcgaa atcatcaagt cgttcaaggg ctggacgacg tacttcaagg gcttccatga
      661 gaaccgcaag aatgtctatt cgtccaacga catcccgacg tcgatcatct accggattgt
      721 cgatgacaac ctccccaaat ttttagaaaa caaggcgaag tacgagtcgc tgaaggataa
      781 ggccccggaa gccatcaact atgagcagat caaaaaagat ttagcggaag agctgacgtt
      841 cgacattgac tacaaaacct ccgaggtgaa tcagcgggtg ttttctttag acgaagtgtt
      901 cgaaatcgcg aacttcaaca actatttaaa tcaatccggc atcaccaaat tcaataccat
      961 tatcggcggc aagttcgtca acggcgagaa cacgaaacgc aaggggatca acgaatacat
     1021 caacctctac tcgcaacaga tcaacgacaa gacgctcaaa aaatataaga tgtcggtcct
     1081 ctttaagcaa atcctctcgg acacggagtc gaaatccttc gtgatcgaca agctggaaga
     1141 tgattccgac gtggtcacca ccatgcagtc cttttacgaa caaatcgccg cctttaagac
     1201 cgtggaggag aaatccatta aggagacgct gtcgttatta ttcgacgatt taaaggccca
     1261 gaagctggac ctctcgaaga tctacttcaa aaacgataag tccctcacgg atctgtccca
     1321 acaagttttt gacgattact ccgtcatcgg caccgcggtg ctggaataca ttacgcagca
     1381 aatcgcgccc aagaatttag acaatccgtc caagaaggag caagaactca tcgccaaaaa
     1441 aactgagaag gccaagtatt tatctttaga gacgattaag ctcgcgctgg aggagttcaa
     1501 caagcatcgc gacattgaca agcagtgccg ctttgaggag atcctcgcga acttcgccgc
     1561 gatccccatg atttttgacg agatcgcgca gaataaagac aatttagccc agatctccat
     1621 caaataccag aatcaaggga agaaggactt attacaagcc tcggccgagg acgatgtcaa
     1681 ggccatcaag gatttactcg atcagaccaa taatttactg cacaagctca agatctttca
     1741 tatctcgcag tcggaggaca aagccaatat tttagacaag gacgagcact tttatctggt
     1801 ttttgaagaa tgctacttcg agctcgcgaa tattgtcccg ctgtacaaca agatccggaa
     1861 ctacatcacc cagaagccct actccgacga gaagtttaag ctgaatttcg agaactcgac
     1921 tttagcgaac ggctgggata aaaacaagga gcccgacaac acggccattc tgtttattaa
     1981 ggacgataag tactatttag gggtgatgaa taagaagaac aacaaaatct ttgacgacaa
     2041 agccattaag gaaaacaaag gggaggggta caagaagatt gtgtataaac tgctgccggg
     2101 ggccaacaaa atgctcccga aagttttttt ctcggcgaaa tcgatcaagt tctataaccc
     2161 gtccgaggac attctgcgca tccggaacca ctccacccat acgaaaaacg gctcgcccca
     2221 gaaggggtac gaaaagttcg agtttaatat cgaggattgc cgcaaattca tcgactttta
     2281 caaacagtcg atctcgaagc acccggaatg gaaagatttc gggttccggt tctcggacac
     2341 gcagcggtac aactcgatcg atgagttcta ccgggaggtc gagaaccaag gttataagct
     2401 caccttcgaa aacatctcgg aatcgtacat tgactccgtg gtcaaccaag ggaaactcta
     2461 cctcttccag atctataata aggacttctc cgcctactcg aagggccgcc cgaatttaca
     2521 caccctctac tggaaagcgc tgttcgacga acggaattta caagatgtgg tctacaagct
     2581 caacggcgag gcggagctct tctaccgcaa gcaatcgatt cccaagaaga ttacccaccc
     2641 cgcgaaggag gcgatcgcga acaagaataa ggacaacccg aagaaggaat cggtttttga
     2701 gtacgatctc atcaaggaca aacggttcac ggaagataag ttcttcttcc actgcccgat
     2761 taccatcaac ttcaagtcct cgggggccaa taagtttaat gacgagatca atttactgct
     2821 gaaggagaag gcgaacgacg tccacatcct ctccattgac cggggggagc ggcatctcgc
     2881 ctattatact ttagtcgacg gcaaggggaa catcatcaag caagacacct tcaacatcat
     2941 cggcaacgat cggatgaaaa ctaattacca tgataagctc gcggcgattg agaaagaccg
     3001 cgactccgcg cggaaggact ggaagaaaat taacaacatc aaggaaatga aagagggcta
     3061 tttatcccaa gttgtccatg agatcgccaa actggtcatc gagtataacg cgatcgtggt
     3121 ttttgaggat ttaaattttg gcttcaaacg tggtcgtttt aaggtcgaga aacaagttta
     3181 tcaaaaatta gaaaagatgc tcatcgaaaa gctcaactac ctcgtcttta aagacaacga
     3241 gtttgacaaa accggcggcg tcctccgcgc gtaccagctg acggcgccct ttgaaacctt
     3301 taaaaagatg ggcaaacaga cgggcatcat ttactacgtc ccggcggggt ttacctcgaa
     3361 aatctgcccg gtgaccggct ttgtcaatca actgtacccc aaatacgagt ccgtctcgaa
     3421 gtcccaagag tttttttcca agttcgacaa gatctgttac aacctcgaca aagggtattt
     3481 tgagttctcc ttcgattaca agaactttgg cgataaggcg gcgaaaggga agtggacgat
     3541 cgcctccttc ggctcccgtt taatcaattt tcggaactcc gataaaaacc acaactggga
     3601 tacccgcgag gtgtatccga ccaaggagct ggagaagctg ctgaaagact attcgattga
     3661 gtatgggcac ggcgagtgta tcaaggcggc catctgcggg gagtccgaca agaagttttt
     3721 cgcgaagctg acctccgtcc tcaacaccat cctccaaatg cggaactcga aaaccggcac
     3781 cgaactggac tatttaatct cccccgtcgc cgacgtcaac ggcaatttct ttgattcccg
     3841 ccaagctccg aagaatatgc cccaagatgc ggacgccaac ggcgcgtacc acatcggttt
     3901 aaaagggctc atgctgctgg gccggattaa gaataaccaa gaagggaaaa agctgaattt
     3961 agtcatcaag aatgaggagt acttcgagtt cgtccagaac cgcaataact gagaattcag
     4021 atctacgcgt tccccgcaaa agcggccttt gactccctgc aagcctcagc gaccgaatat
     4081 atcggttatg cgtgggcgat ggttgttgtc attgtcggcg caactatcgg tatcaagctg
     4141 tttaagaaat tcacctcgaa agcaagctga taaaccgata caattaaagg ctccttttgg
     4201 agcctttttt tgctgctcct tcggtcggac gtgcgtctac gggcacctta ccgcagccgt
     4261 cggctgtgcg acacggacgg atcgggcgaa ctggccgatg ctgggagaag cgcgctgctg
     4321 tacggcgcgc accgggtgcg gagcccctcg gcgagcggtg tgaaacttct gtgaatggcc
     4381 tgttcggttg ctttttttat acggctgcca gataaggctt gcagcatctg ggcggctacc
     4441 gctatgatcg gggcgttcct gcaattctta gtgcgagtat ctgaaagggg atacgcaagt
     4501 cttccggtgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     4561 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     4621 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     4681 tcactaaagg gaacaaaagc tggaggacct cgaggggggg cccggtaccc aattcgccct
     4741 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     4801 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     4861 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     4921 gggacgcgcc ctgtagcggc tgagaagaca tactccatct ggatttgttc agaacgctcg
     4981 gttgccgccg ggcgtttttt atctagacga aaagtgccac ctgggtcctt ttcatcacgt
     5041 gctataaaaa taattataat ttaaattttt taatataaat atataaatta aaaatagaaa
     5101 gtaaaaaaag aaattaaaga aaaaatagtt tttgttttcc gaagatgtaa aagactctag
     5161 ggggatcgcc aacaaatact accttttatc ttgctcttcc tgctctcagg tattaatgcc
     5221 gaattgtttc atcttgtctg tgtagacgac cacacacgaa aatcctgtga ttttacattt
     5281 tacttatcgt taatcgaatg tatatctatt taatctgctt ttcttgtcta ataaatatat
     5341 atgtaaagta cgctttttgt tgaaattttt taaacctttg tttatttttt tttcttcatt
     5401 ccgtaactct tctaccttct ttatttactt tctaaaatcc aaatacaaaa cataaaaata
     5461 aataaacaca gagtaaattc ccaaattatt ccatcattaa aagatacgag gcgcgtgtaa
     5521 attacaggca agcgatccgt ctaagaaacc attattatca tgacattaac ctataaaaat
     5581 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
     5641 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     5701 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
     5761 tcagagcaga ttgtactgag agtgcaccat accacctttt caattcatca tttttttttt
     5821 attctttttt ttgatttcgg tttccttgaa atttttttga ttcggtaatc tccgaacaga
     5881 aggaagaacg aaggaaggag cacagactta gattggtata tatacgcata tgtagtgttg
     5941 aagaaacatg aaattgccca gtattcttaa cccaactgca cagaacaaaa acctgcagga
     6001 aacgaagata aatcatgtcg aaagctacat ataaggaacg tgctgctact catcctagtc
     6061 ctgttgctgc caagctattt aatatcatgc acgaaaagca aacaaacttg tgtgcttcat
     6121 tggatgttcg taccaccaag gaattactgg agttagttga agcattaggt cccaaaattt
     6181 gtttactaaa aacacatgtg gatatcttga ctgatttttc catggagggc acagttaagc
     6241 cgctaaaggc attatccgcc aagtacaatt ttttactctt cgaagataga aaatttgctg
     6301 acattggtaa tacagtcaaa ttgcagtact ctgcgggtgt atacagaata gcagaatggg
     6361 cagacattac gaatgcacac ggtgtggtgg gcccaggtat tgttagcggt ttgaagcagg
     6421 cggcagaaga agtaacaaag gaacctagag gccttttgat gttagcagaa ttgtcatgca
     6481 agggctccct atctactgga gaatatacta agggtactgt tgacattgcg aagagcgaca
     6541 aagattttgt tatcggcttt attgctcaaa gagacatggg tggaagagat gaaggttacg
     6601 attggttgat tatgacaccc ggtgtgggtt tagatgacaa gggagacgca ttgggtcaac
     6661 agtatagaac cgtggatgat gtggtctcta caggatctga cattattatt gttggaagag
     6721 gactatttgc aaagggaagg gatgctaagg tagagggtga acgttacaga aaagcaggct
     6781 gggaagcata tttgagaaga tgcggccagc aaaactaaaa aactgtatta taagtaaatg
     6841 catgtatact aaactcacgg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6901 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6961 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     7021 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     7081 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     7141 gggctgtgtg cacgaccccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     7201 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     7261 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     7321 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     7381 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     7441 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     7501 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     7561 attatcaaaa aggatcttca cctagatcct tttggttcat gtgcagctcc atcagcaaaa
     7621 ggggatgata agtttatcac caccgactat ttgcaacagt gccgttgatc gtgctatgat
     7681 cgactgatgt catcagcggt ggagtgcaat gtcgtgcaat acgaatggcg aaaagccgag
     7741 ctcatcggtc agcttctcaa ccttggggtt acccccggcg gtgtgctgct ggtccacagc
     7801 tccttccgta gcgtccggcc cctcgaagat gggccacttg gactgatcga ggccctgcgt
     7861 gctgcgctgg gtccgggagg gacgctcgtc atgccctcgt ggtcaggtct ggacgacgag
     7921 ccgttcgatc ctgccacgtc gcccgttaca ccggaccttg gagttgtctc tgacacattc
     7981 tggcgcctgc caaatgtaaa gcgcagcgcc catccatttg cctttgcggc agcggggcca
     8041 caggcagagc agatcatctc tgatccattg cccctgccac ctcactcgcc tgcaagcccg
     8101 gtcgcccgtg tccatgaact cgatgggcag gtacttctcc tcggcgtggg acacgatgcc
     8161 aacacgacgc tgcatcttgc cgagttgatg gcaaaggttc cctatggggt gccgagacac
     8221 tgcaccattc ttcaggatgg caagttggta cgcgtcgatt atctcgagaa tgaccactgc
     8281 tgtgagcgct ttgccttggc ggacaggtgg ctcaaggaga agagccttca gaaggaaggt
     8341 ccagtcggtc atgcctttgc tcggttgatc cgctcccgcg acattgtggc gacagccctg
     8401 ggtcaactgg gccgagatcc gttgatcttc ctgcatccgc cagaggcggg atgcgaagaa
     8461 tgcgatgccg ctcgccagtc gattggctga gctcatgagc ggagaacgag atgacgttgg
     8521 aggggcaagg tcgcgctgat tgctggggca acacgtggag cggatcgggg attgtctttc
     8581 ttcagctcgc tgatgatatg ctgacgctca atgccgtttg gcctccgact aacgaaaatc
     8641 ccgcatttgg acggctgatc cgattggcac ggcggacggc gaatggcgga gcagacgctc
     8701 gtccgggggc aatgagatat gaaaaagcct gaactcaccg cgacgtatcg atgtcgaggt
     8761 tcctcagggg agccacccca gagaagccct cggagctgag cggagctatt tccaaagcca
     8821 tgccagctag agacagtgca caccgccaag catctgcaaa accctcgcct ggagggaaag
     8881 tgcaatgtac gtacgctggt ttccctccag aaagggattc tgcggcttct caactttgga
     8941 agaagaggcg gaacgaactg ctgtttcagc ctactcatgt gaggaggctg gtcctttacc
     9001 ctgatgcccg agggcatcag ggtaaaggac cagcctcgcc aactaggagc gctaccaagg
     9061 cgaacacaac ccaggacttg gtagaaccct tcgccggaga agttcagact catcggcatg
     9121 agcacccctc gccgcgcact cggcaccggt ccgtcgacca ccaccaggac gtcgttgtcg
     9181 acgtcggccc cgcggctcct gcccgccgaa cgcgtcgtcg tcgacggcct ggtgctcatc
     9241 gacgagcacc cggagccagg tgaaaagcgc cggcggacgc tcggactggg cgcgggattc
     9301 cagcagtaac ccaggtccgc cggccacctc acggcaggca gaccctcggc tttcgcgccg
     9361 ggaccgccat gagaccgcca cccggatgtc cggggtggcg gtctcatggc ggtccctcag
     9421 cggccctacg cgaccgctgt gtcgacgcgg aggcagtctc cggggtgctg gccccagggc
     9481 tggagtaccg gggcgagctt gcccttgcag cggcggcaca cgggcgacgc ggcgccgggc
     9541 gggggagtct tgacgtcgac ggtgaccggc ttcggcttca ggcgcttccg aaggctgatc
     9601 gtcgggcgga tcgcctcctt cttcggctga cgcactcggt cgagcgaagc ggccagctcc
     9661 tccgtgcggg cctcgttggc cttccggcgc gcctcgcgga aagcagcttc ctcctcggac
     9721 atgacctcga acctcatctg gtcagcgtca aggtcgcccg gcgcggccgg cgcttccggg
     9781 gcgggcgggt ccaggacgtc cttgccccac accaggcccc aggactcgac gagccgccgg
     9841 acgcccggta ggccgtacgt ctcggcgacc ttgatgaggt cgaggcgacg tccggcgacg
     9901 cgggcgatgt atcggtacca gatgtaggcc gggatgaccg cgatggcgac caggccctcg
     9961 gtgtcgtcgg tgatctcctc ctcggtgcgg acgtcctgct ggatgccgag ttccttgatc
    10021 agccggttca ggttctgcga ccggtagtgc ttgcggacct ggaacacgcc gaactcgcgc
    10081 tcgcggtact tctcgacgaa cgggccgggc cgacgaagcc gctgcagctc ggcggccgcc
    10141 gcgtcgccca ggtcgagcgg tcccatgcgg tcgtcgccgc gaccggcctt gaagttctgt
    10201 ccggccagct ccaggccgat cttggcgacg ccgcccttgg tcttgtcgcc gtccttgtag
    10261 aggtagcggg cctgcttgcc cgcatcgccg tcagcggcgt ccgcgccgtt gagtgggcgc
    10321 acgtcggtgc cgtggccctt gccctcacag gagcaaccgg gccggtcgca cgtctcgctg
    10381 acggtgtagc cgcccgcgga ttcgaccccg gcggcccagg ctccggcgag tgcgtcgcgg
    10441 aacgtggcct gggcgtccgg gccgagcacc tcgcgggtga cccagagcgt gtgccagtgc
    10501 aggtgccagc cggagcccca gccgaaggtg tcctcgaagg cccgctcgta gccgatgatc
    10561 ccgaagtcgt cgcgcatcgt gcgccagcgg cggccggacg agccgtacgc gcccttccag
    10621 ccgtcgtgca agaccgcgac caggccgtgc cgcattccct tgcggacggt gccgaacgcc
    10681 atgcgctcga agtggcgcaa cgtgttcgtg ccaaggtgca gcccgtaccc ggcgtccgcg
    10741 agaccgtcgg cggcgagctg cacgttcgag ccccgtacgg ccaggatgcg gctcatgcac
    10801 cacgggcagg tgtggacgtt gttgcagcgg cacgtgttgc cccacgtcgc ctcgcccggc
    10861 ttccacatca gctcggccgt cccggcagtg agccgggtcc cgcagccctt gaacgcctcg
    10921 ttcagcgaca ccgtctggtg ccggtcccgc cgggcgaacc gctcgtcgcg cgggtcctcc
    10981 cgcccggctg tcgcggcacc ctcgtttggg gtagaacccg ttccagttac agcgctctga
    11041 cctgcagtgg acggagattt tcccttacta ctaaagcccg cgtccggatt acccgctgta
    11101 gtcgtgcttg ctacgctgcg tgactggtcc gcaatgagac gctttgcgcg ctttcggcag
    11161 gcgtccgagc agtagatttt ggggcgcttc ccggggatgt ggacgatcgg ggtgccgcag
    11221 tggcacttcg gtccggcggg gcgcggtggt gtcgacgcgc tgttctctcg tacgctcgtc
    11281 acagagcaaa cgtcctcact cggcatgctg cgccggttcg ggggcggcga gcccgggagg
    11341 ccaatcccgg gctcgtgcca tttctgggtc ctgttgatcc tggcattggt gtggccgttc
    11401 attgcccctg ctcgctcctg acgcgccgat agacgtccga tacgcccggt gctggtggga
    11461 tttgataggt cggaagaagc cccgcccggg cctgggcggg gcttcctgtg cgtcaggacc
    11521 tcctcgtcgt gagcctcttc ggcctatgga cggagtgacc tcgtgatccg ttacagccgc
    11581 gcgcgctcgc gtagagcggt ctcatcagtt ccacgaacgg tcctcttcgc agatcagggc
    11641 gttggggcgg agtctcacca aggactacgt ctgctggcga tttccgttac accccgggcg
    11701 gtggccggcg cacacgcgcg cccgcgttgg gcagtgcaga aagtgcagaa acctaggcgc
    11761 tgatggtcca ggtccacggt tcgtcgtcgg cggcggcgcg ggcggcggcg tcggccaggg
    11821 cgcgggcgag accggctacg gcgggcttga tgcgccggtt gcgggcgacc ttgagcagct
    11881 agtatgcagg tcgacggatc ttttccgctg cataaccctg cttcggggtc attatagcga
    11941 ttttttcggt atatccatcc tttttcgcac gatatacagg attttgccaa agggttcgtg
    12001 tagactttcc ttggtgtatc caacggcgtc agccgggcag gataggtgaa gtaggcccac
    12061 ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca cctggcggtg ctcaacggga
    12121 atcctgctct gcgaggctgg ccggctaccg ccggcgtaac agatgagggc aagcggatgg
    12181 ctgatgaaac caagccaacc aggaagggcg gcccacctat caaggtgtac tgccttccag
    12241 acgaacgaag agcgattgag gaaaaggcgg cggcggccgg catgagcctg tcggcctacc
    12301 tgctggccgt cggccagggc tacaaaatca cgggcgtcgt ggactatgag cacgtccgcg
    12361 agctg
//
