LOCUS       sequence-352017-       10982 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of mouse Tyw1-V5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10982)
  TITLE     Gerace lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV Tyw1-V5
FEATURES             Location/Qualifiers
     source          1..10982
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2221..2432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2433..3371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3328..3348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3402..3411
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5598..5639
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5776..6325
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5920..5937)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6147..6166
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6328..6927
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6328..6347)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6824..6844
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6951..7539
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7004..7024)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7422..7433)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7746..8379
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8507..8523)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8507..8523)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8520..8542)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8531..8547
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8555..8561,8562..8579,8580..8585))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8600..8621
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8738..8755)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8909..9497)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8989..9008)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9668..10459,10460..10528))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10291..10310
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10529..10633)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10681..10815
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10718..10737)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10772..10791
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccgcca tcggcccgcc cgccgccatg ggtcctctgg
     3421 atgtgtggga cttgtcgcct ttgctgtcac tgtggatgaa caggttctac atatatatgg
     3481 gctgtgccct gggccttacc ctttgcatct gtgtccagat catcaagaag caggtcactc
     3541 gttcccagga gaaacgtgtc ccaggagctc ctgatagctc cctctcccct cagaagaagc
     3601 agacacacgt ttctggagtg aagatttttt acggatctca gacgggcact gccaagggat
     3661 ttgcagtggt tcttgccaaa gctgtcacct ctctggacct gcctgtggcc attattaatc
     3721 tgaaggaata tgatccagat gacagtctga taggagagat cactagtaaa accgtctgtg
     3781 ccttcctggt cgctacgtac acggacggct gccctacaga gagtgcagag tggttctgca
     3841 agtggttaga ggaatcagct aatgatttcc gctttggcaa aacttacctg aagggacttc
     3901 gctatgcagt gtttggcctg ggagactctg cctaccggag ccacttcaac aaggtcagca
     3961 cgaacgttga caaatggctc tggatgctcg gtgcccagcg ggtgctgacc cgaggggaag
     4021 gcgactgcaa tgcagttcag agcaaacatg gcagcatcga ggccgacttc acagcctgga
     4081 agaccaagtt catctcccgg ctgcaggccc tgcagagagg ggagaaaaag gcttgtggcg
     4141 ggaactgcaa gagagggaag tgtgagtcgg cccagcatgg cccaggagag gcgcggcctc
     4201 acccgcaagg cgaattgcat cctggggatg ctgaggagga agagccctgt gagagcagca
     4261 gcgaggacga gctcgggacc caggattatc agagcctgac ttctgtggtg gatgttgagg
     4321 acctgggcaa catcatgaac cccgtgaaga gggaaaagag agaaaagtct catcaggacg
     4381 ggaaagctgc catgcagagg aacccagaga agactgagga cggcgaagga agagctatga
     4441 taacacctgc tctgcgggaa gccctgacta aacaagggta ccagctgatt ggcagccact
     4501 ctggtgtgaa gctttgcagg tggacaaagt cgatgctgcg ggggagagga ggttgctaca
     4561 aacacacatt ctacggcatt gagagccacc gctgcatgga agccacccca agcttggcgt
     4621 gtgcgaataa atgtgtcttc tgctggcggc accataccaa tcctgtgggc actgagtggc
     4681 ggtggaagat ggaccagcca gaattgatct tgaaggaagc cattgagaac catcagaaca
     4741 tgattaaaca gttcaaaggc gtgccaggcc tcaaggcgga gcgctttgaa gaggggatgg
     4801 aggtcaagca ctgtgcgctg tcactcgtgg gtgagccgat catgtaccca gaaatcaaca
     4861 ggcttttgaa gctgctccac cagcacggca tctccagctt ccttgtcacc aatgcgcagt
     4921 tccctgagga gatcaggaag ctcacaccag ttactcagct ctacgtgagc gtggacgcca
     4981 gcaccagaga cggcctgaag aagatcgacc gcccgctctt caaggatttc tggcagcgct
     5041 tcctagacag tttgaaagcc ttatctgcaa agcaacaacg aacggtctac agactgacgc
     5101 ttgtgaagtg ttggaacgtg gatgaactcc aggcctacgc tgagctggtg tccttgggga
     5161 accccgactt catcgaggtc aagggtgtga cctactgtgg tgagagtgca gccagcagcc
     5221 tcaccatggc caatgtccct tggcacgagg aagtggttcg gtttgtccgt gagctggtcg
     5281 atctgcttcc cgactatgaa gttgcgtgtg aacacgagca ctccaactgc ctcctgatcg
     5341 gacacaagaa gtttaagatc gatggagaat ggtggacatg gatcaattac agccgcttcc
     5401 aagagctcgt tctgcagtac gaagagagtg gaggctccaa gacgttcagc tcgagggatt
     5461 acatggccag aaccccacag tgggcactgt ttggtgccag ggaaagaggc tttgatccca
     5521 aggacacgag ataccagagg aagaacaaaa cgaaggacat ttccggatgt ggtgggccgg
     5581 gcgggggtgg cgggggaggt aagcctatcc ctaaccctct cctcggtctc gattctacgt
     5641 gagtttaaac ttcgaaacgc gtgaattcct cgagggcggc cgctctagag tcgacgggcc
     5701 gcggtaacaa ttgttaacta acttaagcta gcaacggttt ccctctagcg ggatcaattc
     5761 cgcccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     5821 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     5881 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     5941 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     6001 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     6061 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     6121 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     6181 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
     6241 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     6301 ttttcctttg aaaaacacga taataccatg accgagtaca agcccacggt gcgcctcgcc
     6361 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     6421 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     6481 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     6541 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     6601 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     6661 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     6721 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     6781 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     6841 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     6901 tgcatgaccc gcaagcccgg tgcctgaccc gggcggggcg cgtctggaac aatcaacctc
     6961 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     7021 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     7081 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     7141 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     7201 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     7261 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     7321 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     7381 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     7441 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     7501 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg
     7561 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
     7621 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
     7681 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag
     7741 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta
     7801 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
     7861 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
     7921 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
     7981 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
     8041 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
     8101 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
     8161 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
     8221 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     8281 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     8341 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     8401 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt
     8461 gctagcgttt accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     8521 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     8581 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     8641 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     8701 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     8761 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     8821 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     8881 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     8941 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     9001 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     9061 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     9121 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     9181 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     9241 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     9301 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     9361 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     9421 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     9481 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     9541 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     9601 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     9661 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     9721 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     9781 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     9841 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     9901 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     9961 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    10021 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    10081 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    10141 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    10201 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    10261 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    10321 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    10381 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    10441 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    10501 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    10561 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    10621 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
    10681 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
    10741 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    10801 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
    10861 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
    10921 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
    10981 gt
//
