LOCUS       sequence-352023-       11743 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of  V5-tagged mouse Syne3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11743)
  TITLE     Gerace lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV V5-Nesprin-3
FEATURES             Location/Qualifiers
     source          1..11743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2221..2432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2433..3371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3328..3348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3387..3396
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3396..3437
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6537..7086
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6681..6698)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6908..6927
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7089..7688
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7089..7108)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7585..7605
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7712..8300
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7765..7785)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8183..8194)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8507..9140
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9268..9284)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9268..9284)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9281..9303)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9292..9308
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9316..9322,9323..9340,9341..9346))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9361..9382
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9499..9516)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9670..10258)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9750..9769)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10429..11220,11221..11289))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11052..11071
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11290..11394)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11442..11576
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11479..11498)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11533..11552
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccgccg ccatgggtaa gcctatccct aaccctctcc
     3421 tcggtctcga ttctacgggg ggcggagggg gtgggcccat gacgcagcag ccccaggagg
     3481 actttgagag gagcgtggag gatgcccagg cctggatgaa ggtgatacag gagcagcttc
     3541 aggtcaatga caacacgaag gggccccgag cggccctgga ggcaaggctt cgagagacag
     3601 agaaaatctg ccagctggag tctgaaggaa tggtgaaggt ggaactggtc ctgcgggcgg
     3661 cggaggccct cttggcaact tgccaggagg gccagaaacc tgagatcctg gcccggctga
     3721 gggatatcaa gtctcagtgg gaggagacgg tcacctacat gacccactgc cacagtcgca
     3781 tcgagtgggt gtggctgcac tggagtgagt acctgctggc ccaggatgag ttttaccgct
     3841 ggttccagaa gatggtggtc gcactggagc cccccgtgga gctgcagctg ggcttgaagg
     3901 agaagcaatg gcagctgagc cacgcccagg tgctgctgca caacgtggac aatcaggctg
     3961 tgctcctgga caggctgttg gaggaggcgg gctccctgtt cagcaggatc ggagacccca
     4021 gcgtggatga agatgcccag aagaggatga aggctgagta cgatgccgtg aaggccagag
     4081 cccagcgcag ggtggacctc ctggcccagg tggcccagga ccatgagcag taccgggagg
     4141 acgtgaatga gttccagctg tggctgaagg cggtggtgga gaaggtgcac agctgtctgg
     4201 ggcggaactg caagctggcc acagaacttc gtctctctac gctgcaggac atcgccaagg
     4261 atttccctag gggtgaggag tctctgaaaa gattggagga acaggctgtg ggtgtcattc
     4321 aaaacacctc tcccttgggt gcagagaaga tctcagggga gctggaggag atgcggggtg
     4381 tcctggagaa gctgagagtc ctctggaaag aggaggaagg gaggctgcgg ggcctgctcc
     4441 agtccagggg ggactgtgag cagcagatcc aacagctgga ggcagaactg ggagacttca
     4501 agaaaagcct tcagaggctg gcccaggagg gcttggagcc cacggtgaag acagccacag
     4561 aggatgagct ggtggcccag tggaggctgt tctcggggac tcgggctgca ctggcttcag
     4621 aggaaccccg tgtagaccgg ttacaaactc aactgaagaa acttgtcacc ttcccggacc
     4681 tgcagtcact ctctgacagc gtggtagcca ccattcagga ataccaaagt atgaagggga
     4741 agaataccag gctccacaat gcgacccggg cagagctgtg gcagcgtttc cagcggcccc
     4801 taaatgacct gcagctgtgg aaggccctgg cccagaggct cctggacatc acggccagcc
     4861 tgcctgacct ggcctccatt cacacctttc taccccagat tgaggcggcc ctcacggaaa
     4921 gctctcgcct gaaagagcag ctggcgatgc tgcagctgaa gaccgacctg ctgggcagca
     4981 tctttggcca ggagagagca gccaccctcc tggagcaggt gacaagttct gtgagggaca
     5041 gagacctact gcataacagc cttcttcagc ggaagagcaa acttcagagc ctgcttgttc
     5101 agcacaagga ctttggggtg gcttttgatc ccctaaacag gaagctccta gacctccagg
     5161 ccaggatcca agcagagaaa gggcttccga gggaccttcc tggaaagcag gtccagctcc
     5221 taaggttgca ggggctgcag gaagaggggc tggatctggg gacacagatc gaggctgtga
     5281 ggcctcttgc ccatgggaac tctaagcacc agcagaaagt agaccagatc tcctgtgacc
     5341 agcaagccct gcagaggtcc ctggaggatc tcgtggacag gtgtcagcag aacgtacggg
     5401 aacattgtac cttcagtcac aggctgtcgg agctgcagct atggatcacc atggccacac
     5461 agacattaga gtcacaccaa ggggatgtgc gtctgtggga tgctgagtcc caagaggctg
     5521 gactcgagac gctgctgtct gaaatcccag agaaagaggt ccaggtgtcc ctgctccaag
     5581 cactgggcca gcttgtgatg aagaagtctt ccccagaagg ggcaaccatg gtccaggagg
     5641 agctgaggaa gctgatggag tcttggcagg ccctgcggct gctagaggag aacatgctga
     5701 gtctcatgag aaaccagcag ctgcagagga cagaggtgga cacggggaag aagcaggtgt
     5761 tcaccaacaa catcccaaag gccggctttc tcatcaaccc tcaggacccc attcccagga
     5821 gacagcatgg ggcaaaccca ctggaaggac acgacctccc tgaagatcat ccccagctcc
     5881 tgagggactt tgaacagtgg ctgcaggcag aaaactccaa gctacgtaga atcatcacaa
     5941 tgagagtggc cacagccaag gacttgagga ccagagaggt gaagctgcag gagctggagg
     6001 cccgaatccc agaaggccag cacctctttg agaacctgct tcgtctcagg ccggcaaggg
     6061 acccctccaa cgagctggaa gatctgcgct accggtggat gctgtacaag tccaagctca
     6121 aggactctgg ccacctgctg accgagagtt ctccggggga gctgactgca ttccagaaga
     6181 gtcggaggca gaagcggtgg agtccctgct ctctcctaca gaaagcatgc cgtgtggcac
     6241 tgccattgca gctgttgctc ctgctctttc tgctgctgct gttcctgctg ccggccggcg
     6301 aggaggagcg cagctgcgcc ctggccaaca acttcgcccg ctcctttgcg ctcatgcttc
     6361 ggtacaatgg ccccccgccc acctagtgat gggccgcgac gatgtttaaa cttcgaaacg
     6421 cgtgaattcc tcgagggcgg ccgctctaga gtcgacgggc cgcggtaaca attgttaact
     6481 aacttaagct agcaacggtt tccctctagc gggatcaatt ccgccccccc cccctaacgt
     6541 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     6601 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     6661 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     6721 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     6781 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     6841 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     6901 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     6961 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     7021 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
     7081 ataataccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     7141 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     7201 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     7261 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     7321 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     7381 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     7441 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     7501 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     7561 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     7621 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     7681 gtgcctgacc cgggcggggc gcgtctggaa caatcaacct ctggattaca aaatttgtga
     7741 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     7801 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     7861 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     7921 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     7981 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     8041 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     8101 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     8161 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     8221 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     8281 cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga
     8341 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     8401 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     8461 aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt
     8521 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     8581 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     8641 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
     8701 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
     8761 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
     8821 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
     8881 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
     8941 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     9001 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     9061 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     9121 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     9181 tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt taccgtcgac
     9241 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     9301 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     9361 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     9421 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     9481 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     9541 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     9601 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     9661 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     9721 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     9781 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     9841 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     9901 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     9961 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    10021 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    10081 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
    10141 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    10201 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    10261 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    10321 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    10381 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    10441 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    10501 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    10561 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    10621 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    10681 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    10741 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    10801 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    10861 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    10921 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    10981 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    11041 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    11101 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    11161 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    11221 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    11281 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    11341 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    11401 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt attgcagctt
    11461 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    11521 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact
    11581 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg
    11641 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
    11701 aaagaaattg tatttgttaa atatgtacta caaacttagt agt
//
